Restriction map for
pF1KE2492
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# NO CUT ENZYMES ( 65 enzymes) #
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AccIII AflII AgeI AhdI Aor51HI
AscI AspEI AviII AvrII BbrPI
BciVI BclI BfrI BglI BlnI
BsaBI BseAI BsiWI BspDI BspEI
BspHI Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI Eam1105I Eco47III
EcoT22I FbaI FseI FspI HpaI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PvuI RcaI RsrII
SacI SacII SalI SexAI SfiI
SfuI SgrAI SpeI SspI SwaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 271 GGTACC G^GTACC
AclI 2872 AACGTT AA^CGTT
AflIII 3078 ACATGT A^C[GA][CT]GT
ApaI 4244 GGGCCC GGGCC^C
AseI 3289 ATTAAT AT^TAAT
AsnI 3289 ATTAAT AT^TAAT
Asp700I 2533 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 271 GGTACC G^GTACC
AspI 617 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 4737 CCTCAGC CCTCAGC(-5/-2)
BsaAI 3532 TACGTA [CT]AC^GT[GA]
BsaI 3362 GAGACC GGTCTC(1/5)
Bsp1407I 4835 TGTACA T^GTACA
BspLU11I 3078 ACATGT A^CATGT
BsrDI 1064 CATTGC GCAATG(2/0)
BsrGI 4835 TGTACA T^GTACA
BssHII 4841 GCGCGC G^CGCGC
BstEII 793 GGTGACC G^GT[ACGT]ACC
BstPI 793 GGTGACC G^GT[ACGT]ACC
DrdI 2707 GACTTCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 793 GGTGACC G^GT[ACGT]ACC
EcoRI 4003 GAATTC G^AATTC
EcoRV 2476 GATATC GAT^ATC
KpnI 271 GGTACC GGTAC^C
PaeR7I 3104 CTCGAG C^TCGAG
PflFI 617 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 285 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3834 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3289 ATTAAT AT^TAAT
Psp1406I 2872 AACGTT AA^CGTT
SapI 575 GCTCTTC GCTCTTC(1/4)
SbfI 4821 CCTGCAGG CCTGCA^GG
ScaI 4988 AGTACT AGT^ACT
SnaBI 3532 TACGTA TAC^GTA
SphI 4688 GCATGC GCATG^C
Sse8387I 4821 CCTGCAGG CCTGCA^GG
SspBI 4835 TGTACA T^GTACA
Tth111I 617 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 285 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 198 TCTAGA T^CTAGA
XhoI 3104 CTCGAG C^TCGAG
XmnI 2533 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AatII 2745 GACGTC GACGT^C
5161 GACGTC
AccI 1359 GTCTAC GT^[AC][GT]AC
4156 GTCTAC
AccII 4719 CGCG CG^CG
4842 CGCG
Alw44I 828 GTGCAC G^TGCAC
4315 GTGCAC
AlwNI 525 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1925 CAGCTGCTG
ApaLI 828 GTGCAC G^TGCAC
4315 GTGCAC
BalI 3336 TGGCCA TGG^CCA
5210 TGGCCA
BamHI 372 GGATCC G^GATCC
3645 GGATCC
BbeI 26 GGCGCC GGCGC^C
2184 GGCGCC
BlpI 891 GCTGAGC GC^T[ACGT]AGC
1129 GCTGAGC
Bpu1102I 891 GCTGAGC GC^T[ACGT]AGC
1129 GCTGAGC
BsaWI 238 TCCGGT [AT]^CCGG[AT]
4958 ACCGGA
BsrBI 1883 GAGCGG CCGCTC(-3/-3)
2333 GAGCGG
BstUI 4719 CGCG CG^CG
4842 CGCG
CelII 891 GCTGAGC GC^T[ACGT]AGC
1129 GCTGAGC
DraIII 4123 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
4879 CACGAGGTG
EagI 159 CGGCCG C^GGCCG
3918 CGGCCG
EclXI 159 CGGCCG C^GGCCG
3918 CGGCCG
Eco52I 159 CGGCCG C^GGCCG
3918 CGGCCG
KasI 26 GGCGCC G^GCGCC
2184 GGCGCC
MluNI 3336 TGGCCA TGG^CCA
5210 TGGCCA
MscI 3336 TGGCCA TGG^CCA
5210 TGGCCA
MvnI 4719 CGCG CG^CG
4842 CGCG
NarI 26 GGCGCC GG^CGCC
2184 GGCGCC
NspI 3078 ACATGT [GA]CATG^[CT]
4688 GCATGC
SfoI 26 GGCGCC GGC^GCC
2184 GGCGCC
SmaI 502 CCCGGG CCC^GGG
3799 CCCGGG
XmaCI 502 CCCGGG C^CCGGG
3799 CCCGGG
XmaI 502 CCCGGG C^CCGGG
3799 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AocI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcgI BcnI BfaI BglII BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII