Restriction map for
pF1KB9962
##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FbaI FseI FspI HaeII
HgaI Hin1I KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NarI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 79 CGCG CG^CG
AflIII 698 ACATGT A^C[GA][CT]GT
AlwNI 1970 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 74 GGGCCC GGGCC^C
AvaI 1891 CCCGAG C^[CT]CG[GA]G
AvaII 567 GGTCC G^G[AT]CC
BbsI 1080 GTCTTC GAAGAC(2/6)
BcgI 1323 GCAGAGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1413 AGATCT A^GATCT
BpuAI 1080 GTCTTC GAAGAC(2/6)
BsaBI 1165 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 1891 CCCGAG C^[CT]CG[GA]G
Bsp1407I 395 TGTACA T^GTACA
BspLU11I 698 ACATGT A^CATGT
BspMI 1433 ACCTGC ACCTGC(4/8)
BsrGI 395 TGTACA T^GTACA
BstUI 79 CGCG CG^CG
DraI 1477 TTTAAA TTT^AAA
DraII 73 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 870 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 23 TGGCCG [CT]^GGCC[GA]
EcoNI 1434 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 73 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 276 GATATC GAT^ATC
EcoT22I 761 ATGCAT ATGCA^T
FauI 77 CCCGC CCCGC(4/6)
HindIII 1558 AAGCTT A^AGCTT
HpaI 207 GTTAAC GTT^AAC
MaeII 499 ACGT A^CGT
MamI 1165 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1035 CACAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 335 CAGCTG C[AC]G^C[GT]G
MvnI 79 CGCG CG^CG
MwoI 1649 GCTGGGACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 767 GCCGGC GCC^GGC
NgoMIV 767 GCCGGC G^CCGGC
NsiI 761 ATGCAT ATGCA^T
PflMI 869 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 335 CAGCTG CAG^CTG
SapI 1940 GCTCTTC GCTCTTC(1/4)
ScaI 1849 AGTACT AGT^ACT
SmlI 984 CTTGAG C^T[CT][GA]AG
SphI 763 GCATGC GCATG^C
SspBI 395 TGTACA T^GTACA
Van91I 869 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 865 CCATCCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 69 CCTGAGG CC^T[ACGT]AGG
1237 CCTGAGG
AspHI 1326 GAGCTC G[AT]GC[AT]^C
1938 GAGCTC
BsgI 448 CTGCAC GTGCAG(16/14)
156 GTGCAG
BsiHKAI 1326 GAGCTC G[AT]GC[AT]^C
1938 GAGCTC
BsmFI 1652 GGGAC GGGAC(10/14)
93 GTCCC
BsrFI 767 GCCGGC [GA]^CCGG[CT]
1076 GCCGGT
BstNI 675 CCAGG CC^[AT]GG
1297 CCAGG
Bsu36I 69 CCTGAGG CC^T[ACGT]AGG
1237 CCTGAGG
CfoI 80 GCGC GCG^C
418 GCGC
Cfr10I 767 GCCGGC [GA]^CCGG[CT]
1076 GCCGGT
Eco81I 69 CCTGAGG CC^T[ACGT]AGG
1237 CCTGAGG
EcoRI 836 GAATTC G^AATTC
1605 GAATTC
EcoRII 675 CCAGG ^CC[AT]GG
1297 CCAGG
EcoT14I 776 CCAAGG C^C[AT][AT]GG
1715 CCTTGG
HaeIII 24 GGCC GG^CC
75 GGCC
HhaI 80 GCGC GCG^C
418 GCGC
HinP1I 80 GCGC G^CGC
418 GCGC
HincII 207 GTTAAC GT[CT]^[GA]AC
1617 GTCAAC
HindII 207 GTTAAC GT[CT]^[GA]AC
1617 GTCAAC
MvaI 675 CCAGG CC^[AT]GG
1297 CCAGG
PstI 1256 CTGCAG CTGCA^G
1305 CTGCAG
SacI 1326 GAGCTC GAGCT^C
1938 GAGCTC
StyI 776 CCAAGG C^C[AT][AT]GG
1715 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BanII BbvI BciVI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstYI Cac8I
Cfr13I DdeI DpnII EarI Fnu4HI
FokI HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI