Restriction map for
pF1KE2462
##################################
# NO CUT ENZYMES ( 187 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaHI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcsI 160 GAATTC [GA]^AATT[CT]
AfaI 138 GTAC GT^AC
ApoI 160 GAATTC [GA]^AATT[CT]
Asp700I 156 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 190 GGATCC G^GATCC
BfaI 5 CTAG C^TAG
BpmI 250 CTCCAG CTGGAG(16/14)
BsaBI 240 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 226 GAGACC GGTCTC(1/5)
BsaWI 89 ACCGGA [AT]^CCGG[AT]
BssKI 231 CCAGG ^CC[ACGT]GG
BstAPI 27 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 231 CCAGG CC^[AT]GG
BstYI 190 GGATCC [GA]^GATC[CT]
Cfr13I 72 GGCCC G^G[ACGT]CC
DpnII 191 GATC ^GATC
DsaI 195 CCGTGG C^C[GA][CT]GG
EaeI 319 CGGCCA [CT]^GGCC[GA]
EcoRI 160 GAATTC G^AATTC
EcoRII 231 CCAGG ^CC[AT]GG
EcoT14I 170 CCAAGG C^C[AT][AT]GG
HinfI 21 GATTC G^A[ACGT]TC
MaeI 5 CTAG C^TAG
MaeIII 311 GTGAC ^GT[ACGT]AC
MamI 240 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 191 GATC ^GATC
MflI 190 GGATCC [GA]^GATC[CT]
MslI 210 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 28 CAGCTG C[AC]G^C[GT]G
MvaI 231 CCAGG CC^[AT]GG
NdeII 191 GATC ^GATC
NlaIII 331 CATG CATG^
NlaIV 190 GGATCC GG[ACGT]^[ACGT]CC
PvuII 28 CAGCTG CAG^CTG
RsaI 138 GTAC GT^AC
Sau3AI 191 GATC ^GATC
Sau96I 72 GGCCC G^G[ACGT]CC
ScrFI 231 CCAGG CC^[ACGT]GG
StyI 170 CCAAGG C^C[AT][AT]GG
TfiI 21 GATTC G^A[AT]TC
Tsp45I 311 GTGAC ^GT[GC]AC
TspRI 210 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 190 GGATCC [GA]^GATC[CT]
XmnI 156 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 191 GATCC GGATC(4/5)
190 GGATC
AlwNI 25 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
28 CAGCTGCTG
BsiYI 41 CCGAGAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
144 CCTGCAGAGGG
BslI 41 CCGAGAAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
144 CCTGCAGAGGG
BsmAI 226 GAGAC GTCTC(1/5)
12 GTCTC
BstF5I 246 CATCC GGATG(2/0)
276 GGATG
DdeI 14 CTCAG C^T[ACGT]AG
119 CTCAG
FauI 74 CCCGC CCCGC(4/6)
48 GCGGG
FokI 246 CATCC GGATG(9/13)
276 GGATG
HapII 90 CCGG C^CGG
264 CCGG
HpaII 90 CCGG C^CGG
264 CCGG
MboII 112 GAAGA GAAGA(8/7)
237 GAAGA
MspI 90 CCGG C^CGG
264 CCGG
PstI 34 CTGCAG CTGCA^G
145 CTGCAG
SfcI 34 CTGCAG C^T[GA][CT]AG
145 CTGCAG
Tsp509I 129 AATT ^AATT
161 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AciI AluI BbvI BsaJI Cac8I
Fnu4HI HaeIII ItaI MnlI MwoI
TseI