Restriction map for
pF1KE3380
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AspEI
AviII BanII BbeI BbrPI BbvCI
BcgI BglI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRV FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KspI MaeII MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflII 754 CTTAAG C^TTAAG
AflIII 2459 ACATGT A^C[GA][CT]GT
AocI 1285 CCTAAGG CC^T[ACGT]AGG
AspI 439 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 410 CTCGAG C^[CT]CG[GA]G
AvaII 832 GGTCC G^G[AT]CC
AvrII 2487 CCTAGG C^CTAGG
BamHI 2517 GGATCC G^GATCC
BciVI 285 GTATCC GTATCC(6/5)
BclI 3299 TGATCA T^GATCA
BcnI 2514 CCGGG CC^[GC]GG
BfrI 754 CTTAAG C^TTAAG
BlnI 2487 CCTAGG C^CTAGG
BsgI 2318 CTGCAC GTGCAG(16/14)
BsiWI 1686 CGTACG C^GTACG
BsmBI 1856 GAGACG CGTCTC(1/5)
BsoBI 410 CTCGAG C^[CT]CG[GA]G
BspLU11I 2459 ACATGT A^CATGT
BspMI 1246 GCAGGT ACCTGC(4/8)
BsrDI 3197 GCAATG GCAATG(2/0)
BsrFI 2597 GCCGGT [GA]^CCGG[CT]
BstXI 132 CCATTCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1285 CCTAAGG CC^T[ACGT]AGG
Cfr10I 2597 GCCGGT [GA]^CCGG[CT]
DraIII 2223 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2149 CCATGG C^C[GA][CT]GG
Eco81I 1285 CCTAAGG CC^T[ACGT]AGG
EcoNI 3341 CCTCCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 884 GAATTC G^AATTC
FbaI 3299 TGATCA T^GATCA
HpaI 2988 GTTAAC GTT^AAC
MspA1I 3173 CAGCGG C[AC]G^C[GT]G
NciI 2514 CCGGG CC^[GC]GG
NcoI 2149 CCATGG C^CATGG
NspI 2459 ACATGT [GA]CATG^[CT]
PaeR7I 410 CTCGAG C^TCGAG
PflFI 439 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1705 GACTACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 2659 AGTACT AGT^ACT
SexAI 3036 ACCTGGT A^CC[AT]GGT
SwaI 2186 ATTTAAAT ATTT^AAAT
Tsp45I 508 GTGAC ^GT[GC]AC
Tth111I 439 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1989 TCTAGA T^CTAGA
XcmI 1032 CCATAAGCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 410 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 2154 GGTACC G^GTACC
2218 GGTACC
AciI 471 GCGG CCGC(-3/-1)
3175 GCGG
AseI 1101 ATTAAT AT^TAAT
2778 ATTAAT
AsnI 1101 ATTAAT AT^TAAT
2778 ATTAAT
Asp718I 2154 GGTACC G^GTACC
2218 GGTACC
AspHI 2264 GTGCTC G[AT]GC[AT]^C
2668 GAGCAC
BalI 593 TGGCCA TGG^CCA
1653 TGGCCA
BsiHKAI 2264 GTGCTC G[AT]GC[AT]^C
2668 GAGCAC
BsmI 2164 GAATGC GAATGC(1/-1)
3310 GAATGC
BspHI 513 TCATGA T^CATGA
3302 TCATGA
EaeI 593 TGGCCA [CT]^GGCC[GA]
1653 TGGCCA
EcoT22I 1981 ATGCAT ATGCA^T
2166 ATGCAT
HapII 2514 CCGG C^CGG
2598 CCGG
HpaII 2514 CCGG C^CGG
2598 CCGG
KpnI 2154 GGTACC GGTAC^C
2218 GGTACC
MluNI 593 TGGCCA TGG^CCA
1653 TGGCCA
MscI 593 TGGCCA TGG^CCA
1653 TGGCCA
MspI 2514 CCGG C^CGG
2598 CCGG
NdeI 1591 CATATG CA^TATG
2798 CATATG
NsiI 1981 ATGCAT ATGCA^T
2166 ATGCAT
PflMI 2222 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3050 CCATATTCTGG
PshBI 1101 ATTAAT AT^TAAT
2778 ATTAAT
RcaI 513 TCATGA T^CATGA
3302 TCATGA
SapI 2665 GAAGAGC GCTCTTC(1/4)
3358 GCTCTTC
SmlI 410 CTCGAG C^T[CT][GA]AG
754 CTTAAG
StuI 1582 AGGCCT AGG^CCT
2383 AGGCCT
TfiI 2272 GATTC G^A[AT]TC
3070 GAATC
Van91I 2222 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3050 CCATATTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BanI BbsI BbvI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII XmnI