Restriction map for
pF1KE1598
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbvCI BcgI BlnI BsaHI BseAI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI BstEII BstPI BstUI Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT22I FseI FspI HaeII
Hin1I HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SpeI
Sse8387I SspI SwaI Tth111I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflIII 4031 ACACGT A^C[GA][CT]GT
AocI 2524 CCTAAGG CC^T[ACGT]AGG
Asp700I 1873 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2519 GTGCTC G[AT]GC[AT]^C
BamHI 151 GGATCC G^GATCC
BbrPI 4097 CACGTG CAC^GTG
BglI 3628 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4139 AGATCT A^GATCT
BlpI 793 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 793 GCTAAGC GC^T[ACGT]AGC
BsaBI 2986 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1907 ACCGGA [AT]^CCGG[AT]
BseRI 885 GAGGAG GAGGAG(10/8)
BsiHKAI 2519 GTGCTC G[AT]GC[AT]^C
BsmBI 4212 GAGACG CGTCTC(1/5)
Bsp1407I 511 TGTACA T^GTACA
BspHI 3851 TCATGA T^CATGA
BsrGI 511 TGTACA T^GTACA
Bst1107I 1915 GTATAC GTA^TAC
BstAPI 2101 GCACTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3234 TTCGAA TT^CGAA
BstZ17I 1915 GTATAC GTA^TAC
Bsu36I 2524 CCTAAGG CC^T[ACGT]AGG
CelII 793 GCTAAGC GC^T[ACGT]AGC
CfoI 3122 GCGC GCG^C
DraII 1040 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 2524 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1040 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 142 GAATTC G^AATTC
EcoRV 454 GATATC GAT^ATC
FauI 2386 GCGGG CCCGC(4/6)
HgaI 2976 GCGTC GACGC(5/10)
HhaI 3122 GCGC GCG^C
HinP1I 3122 GCGC G^CGC
MamI 2986 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2843 CCATGG C^CATGG
NspV 3234 TTCGAA TT^CGAA
PflMI 1206 CCAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4097 CACGTG CAC^GTG
PmlI 4097 CACGTG CAC^GTG
PpuMI 1040 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 2872 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3061 CAGCTG CAG^CTG
RcaI 3851 TCATGA T^CATGA
SfuI 3234 TTCGAA TT^CGAA
SphI 3863 GCATGC GCATG^C
SspBI 511 TGTACA T^GTACA
StuI 3918 AGGCCT AGG^CCT
Van91I 1206 CCAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2317 TCTAGA T^CTAGA
XmnI 1873 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 903 GTCTAC GT^[AC][GT]AC
1915 GTATAC
AflII 1345 CTTAAG C^TTAAG
1426 CTTAAG
AseI 2482 ATTAAT AT^TAAT
4260 ATTAAT
AsnI 2482 ATTAAT AT^TAAT
4260 ATTAAT
BanI 2630 GGCACC G^G[CT][GA]CC
3253 GGCACC
BanII 2061 GGGCTC G[GA]GC[CT]^C
2603 GGGCTC
BciVI 2859 GGATAC GTATCC(6/5)
1746 GTATCC
BclI 1053 TGATCA T^GATCA
3065 TGATCA
BcnI 2130 CCGGG CC^[GC]GG
4126 CCCGG
BfrI 1345 CTTAAG C^TTAAG
1426 CTTAAG
BsaI 517 GAGACC GGTCTC(1/5)
3570 GAGACC
BsgI 26 CTGCAC GTGCAG(16/14)
61 GTGCAG
BsmFI 1040 GGGAC GGGAC(10/14)
2870 GGGAC
BspMI 406 GCAGGT ACCTGC(4/8)
2816 GCAGGT
BsrDI 1992 CATTGC GCAATG(2/0)
2833 CATTGC
BstXI 1651 CCAGTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3503 CCAATAAAATGG
DraI 942 TTTAAA TTT^AAA
1645 TTTAAA
DsaI 832 CCACGG C^C[GA][CT]GG
2843 CCATGG
FbaI 1053 TGATCA T^GATCA
3065 TGATCA
HindIII 2893 AAGCTT A^AGCTT
3407 AAGCTT
MslI 1992 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2512 CATTACGGTG
NciI 2130 CCGGG CC^[GC]GG
4126 CCCGG
PshBI 2482 ATTAAT AT^TAAT
4260 ATTAAT
SapI 1600 GAAGAGC GCTCTTC(1/4)
3819 GAAGAGC
SnaBI 1918 TACGTA TAC^GTA
2648 TACGTA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MspA1I MspI MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII