Restriction map for
pF1KE3561
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
Asp700I Asp718I AspEI AvaI AvaII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BlnI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FauI
FseI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 2624 GCGG CCGC(-3/-1)
AflII 1543 CTTAAG C^TTAAG
ApaI 1712 GGGCCC GGGCC^C
AseI 2454 ATTAAT AT^TAAT
AsnI 2454 ATTAAT AT^TAAT
AspI 2034 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1090 TGCGCA TGC^GCA
BalI 1778 TGGCCA TGG^CCA
BamHI 2239 GGATCC G^GATCC
BanI 2257 GGCACC G^G[CT][GA]CC
BanII 1712 GGGCCC G[GA]GC[CT]^C
BclI 248 TGATCA T^GATCA
BfrI 1543 CTTAAG C^TTAAG
BglII 2688 AGATCT A^GATCT
BlpI 1219 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1219 GCTGAGC GC^T[ACGT]AGC
BsaBI 630 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2233 GAGGAG GAGGAG(10/8)
BsgI 1027 CTGCAC GTGCAG(16/14)
Bsp1407I 307 TGTACA T^GTACA
BspLU11I 1972 ACATGT A^CATGT
BspMI 25 ACCTGC ACCTGC(4/8)
BsrBI 2622 GAGCGG CCGCTC(-3/-3)
BsrFI 2430 ACCGGC [GA]^CCGG[CT]
BsrGI 307 TGTACA T^GTACA
CelII 1219 GCTGAGC GC^T[ACGT]AGC
CfoI 1091 GCGC GCG^C
Cfr10I 2430 ACCGGC [GA]^CCGG[CT]
DraI 1407 TTTAAA TTT^AAA
DraII 1712 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1728 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1327 CCATGG C^C[GA][CT]GG
EaeI 1778 TGGCCA [CT]^GGCC[GA]
EcoO109I 1712 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1377 GAATTC G^AATTC
FbaI 248 TGATCA T^GATCA
FspI 1090 TGCGCA TGC^GCA
HhaI 1091 GCGC GCG^C
HinP1I 1091 GCGC G^CGC
MaeII 707 ACGT A^CGT
MamI 630 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1778 TGGCCA TGG^CCA
MscI 1778 TGGCCA TGG^CCA
NcoI 1327 CCATGG C^CATGG
NheI 2055 GCTAGC G^CTAGC
PflFI 2034 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2454 ATTAAT AT^TAAT
PstI 515 CTGCAG CTGCA^G
SphI 2360 GCATGC GCATG^C
SspBI 307 TGTACA T^GTACA
SspI 1839 AATATT AAT^ATT
SwaI 1406 ATTTAAAT ATTT^AAAT
TaqI 2757 TCGA T^CGA
Tth111I 2034 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2757 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 707 ACGTGT A^C[GA][CT]GT
1972 ACATGT
AspHI 1429 GTGCTC G[AT]GC[AT]^C
1725 GAGCAC
BbsI 154 GAAGAC GAAGAC(2/6)
1498 GTCTTC
BcnI 97 CCGGG CC^[GC]GG
1880 CCGGG
BglI 1153 GCCTACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2278 GCCATCCTGGC
BpuAI 154 GAAGAC GAAGAC(2/6)
1498 GTCTTC
BsiHKAI 1429 GTGCTC G[AT]GC[AT]^C
1725 GAGCAC
BsmFI 99 GGGAC GGGAC(10/14)
1211 GGGAC
BsmI 1573 GAATGC GAATGC(1/-1)
4 GCATTC
BspHI 132 TCATGA T^CATGA
1190 TCATGA
BssSI 1938 CACGAG CACGAG(-5/-1)
1295 CTCGTG
Cfr13I 1712 GGGCC G^G[ACGT]CC
1713 GGCCC
EarI 856 CTCTTC CTCTTC(1/4)
986 GAAGAG
EcoT22I 1075 ATGCAT ATGCA^T
1122 ATGCAT
HincII 915 GTCAAC GT[CT]^[GA]AC
1610 GTCAAC
HindII 915 GTCAAC GT[CT]^[GA]AC
1610 GTCAAC
HphI 2193 GGTGA GGTGA(8/7)
600 TCACC
Ksp632I 856 CTCTTC CTCTTC(1/4)
986 GAAGAG
NciI 97 CCGGG CC^[GC]GG
1880 CCGGG
NdeI 1094 CATATG CA^TATG
2715 CATATG
NsiI 1075 ATGCAT ATGCA^T
1122 ATGCAT
RcaI 132 TCATGA T^CATGA
1190 TCATGA
Sau96I 1712 GGGCC G^G[ACGT]CC
1713 GGCCC
SmlI 1543 CTTAAG C^T[CT][GA]AG
2548 CTCAAG
XcmI 180 CCAGGAGTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
368 CCAGAGAAACTGTGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII