Restriction map for
pF1KE2349
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
BamHI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MaeII MamI MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 805 GGTACC G^GTACC
AccI 190 GTAGAC GT^[AC][GT]AC
AccII 182 CGCG CG^CG
AfaI 806 GTAC GT^AC
AlwI 813 GGATC GGATC(4/5)
ApaI 25 GGGCCC GGGCC^C
Asp718I 805 GGTACC G^GTACC
AvaI 21 CCCGGG C^[CT]CG[GA]G
AvrII 534 CCTAGG C^CTAGG
BbeI 338 GGCGCC GGCGC^C
BbvCI 589 GCTGAGG CCTCAGC(-5/-2)
BglI 18 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 534 CCTAGG C^CTAGG
BlpI 612 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 612 GCTCAGC GC^T[ACGT]AGC
BsaI 630 GAGACC GGTCTC(1/5)
BsgI 752 CTGCAC GTGCAG(16/14)
BsmAI 630 GAGAC GTCTC(1/5)
BsoBI 21 CCCGGG C^[CT]CG[GA]G
BspMI 317 ACCTGC ACCTGC(4/8)
BstAPI 9 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 376 GGTGACC G^GT[ACGT]ACC
BstPI 376 GGTGACC G^GT[ACGT]ACC
BstUI 182 CGCG CG^CG
BstYI 813 GGATCT [GA]^GATC[CT]
CelII 612 GCTCAGC GC^T[ACGT]AGC
DraIII 179 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 522 GAAGAG CTCTTC(1/4)
EcoNI 710 CCTCTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 376 GGTGACC G^GT[ACGT]ACC
HaeII 338 GGCGCC [GA]GCGC^[CT]
HgaI 288 GCGTC GACGC(5/10)
HinfI 577 GACTC G^A[ACGT]TC
KasI 338 GGCGCC G^GCGCC
KpnI 805 GGTACC GGTAC^C
Ksp632I 522 GAAGAG CTCTTC(1/4)
MflI 813 GGATCT [GA]^GATC[CT]
MspA1I 266 CAGCTG C[AC]G^C[GT]G
MvnI 182 CGCG CG^CG
NarI 338 GGCGCC GG^CGCC
PleI 577 GACTC GAGTC(4/5)
PpuMI 830 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 266 CAGCTG CAG^CTG
RsaI 806 GTAC GT^AC
SapI 522 GAAGAGC GCTCTTC(1/4)
SfoI 338 GGCGCC GGC^GCC
SmaI 21 CCCGGG CCC^GGG
SpeI 778 ACTAGT A^CTAGT
TaqI 50 TCGA T^CGA
TthHB8I 50 TCGA T^CGA
XhoII 813 GGATCT [GA]^GATC[CT]
XmaCI 21 CCCGGG C^CCGGG
XmaI 21 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 287 GGCGTC G[GA]^CG[CT]C
338 GGCGCC
AspHI 261 GAGCAC G[AT]GC[AT]^C
434 GTGCTC
AvaII 719 GGACC G^G[AT]CC
831 GGACC
BsaHI 287 GGCGTC G[GA]^CG[CT]C
338 GGCGCC
BsiHKAI 261 GAGCAC G[AT]GC[AT]^C
434 GTGCTC
BsmFI 567 GTCCC GGGAC(10/14)
782 GTCCC
BstXI 346 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
431 CCAGTGCTCTGG
DpnII 249 GATC ^GATC
814 GATC
HapII 22 CCGG C^CGG
217 CCGG
Hin1I 287 GGCGTC G[GA]^CG[CT]C
338 GGCGCC
HpaII 22 CCGG C^CGG
217 CCGG
MboI 249 GATC ^GATC
814 GATC
MboII 522 GAAGA GAAGA(8/7)
816 TCTTC
MspI 22 CCGG C^CGG
217 CCGG
NdeII 249 GATC ^GATC
814 GATC
PstI 228 CTGCAG CTGCA^G
236 CTGCAG
Sau3AI 249 GATC ^GATC
814 GATC
StuI 224 AGGCCT AGG^CCT
670 AGGCCT
XcmI 749 CCACTGCACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
884 CCATCCTGACCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwNI BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HphI ItaI
MaeI MaeIII MluNI MnlI MscI
MslI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI