Restriction map for
pF1KE2391
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbrPI BbvCI BcgI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstUI
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoRI
EcoRV EcoT22I FbaI FspI HindIII
HinfI KpnI KspI MaeI MamI
MfeI MluI MroI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 280 GAATTT [GA]^AATT[CT]
AflIII 1079 ACATGT A^C[GA][CT]GT
Aor51HI 1541 AGCGCT AGC^GCT
ApaI 1115 GGGCCC GGGCC^C
ApoI 280 GAATTT [GA]^AATT[CT]
Asp700I 1044 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1235 GGCGCC GGCGC^C
BciVI 1144 GGATAC GTATCC(6/5)
BcnI 312 CCCGG CC^[GC]GG
BglII 371 AGATCT A^GATCT
BsiEI 646 CGGCCG CG[GA][CT]^CG
BsmI 270 GAATGC GAATGC(1/-1)
BspLU11I 1079 ACATGT A^CATGT
BspMI 429 ACCTGC ACCTGC(4/8)
Bst1107I 519 GTATAC GTA^TAC
BstEII 957 GGTCACC G^GT[ACGT]ACC
BstPI 957 GGTCACC G^GT[ACGT]ACC
BstZ17I 519 GTATAC GTA^TAC
EagI 646 CGGCCG C^GGCCG
EclXI 646 CGGCCG C^GGCCG
Eco47III 1541 AGCGCT AGC^GCT
Eco52I 646 CGGCCG C^GGCCG
EcoNI 546 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 957 GGTCACC G^GT[ACGT]ACC
FseI 647 GGCCGGCC GGCCGG^CC
HpaI 1336 GTTAAC GTT^AAC
KasI 1235 GGCGCC G^GCGCC
MseI 1337 TTAA T^TAA
NarI 1235 GGCGCC GG^CGCC
NciI 312 CCCGG CC^[GC]GG
NspI 1079 ACATGT [GA]CATG^[CT]
PflMI 224 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1511 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1431 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1468 CTGCAG CTGCA^G
SbfI 1467 CCTGCAGG CCTGCA^GG
ScaI 1166 AGTACT AGT^ACT
SexAI 794 ACCTGGT A^CC[AT]GGT
SfaNI 556 GATGC GCATC(5/9)
SfcI 1468 CTGCAG C^T[GA][CT]AG
SfoI 1235 GGCGCC GGC^GCC
Sse8387I 1467 CCTGCAGG CCTGCA^GG
Tru9I 1337 TTAA T^TAA
Van91I 224 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1044 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 397 GTCTAC GT^[AC][GT]AC
519 GTATAC
AcyI 1235 GGCGCC G[GA]^CG[CT]C
1431 GACGCC
AfaI 417 GTAC GT^AC
1167 GTAC
BpmI 343 CTGGAG CTGGAG(16/14)
1054 CTGGAG
BsaHI 1235 GGCGCC G[GA]^CG[CT]C
1431 GACGCC
BsaI 211 GAGACC GGTCTC(1/5)
1057 GAGACC
BsmAI 211 GAGAC GTCTC(1/5)
1057 GAGAC
BstXI 902 CCACATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
962 CCATGGTTGTGG
BstYI 371 AGATCT [GA]^GATC[CT]
1460 GGATCT
HaeII 1235 GGCGCC [GA]GCGC^[CT]
1541 AGCGCT
HgaI 1431 GACGC GACGC(5/10)
891 GCGTC
Hin1I 1235 GGCGCC G[GA]^CG[CT]C
1431 GACGCC
MflI 371 AGATCT [GA]^GATC[CT]
1460 GGATCT
NaeI 648 GCCGGC GCC^GGC
1253 GCCGGC
NcoI 908 CCATGG C^CATGG
962 CCATGG
NgoMIV 648 GCCGGC G^CCGGC
1253 GCCGGC
RsaI 417 GTAC GT^AC
1167 GTAC
SacI 121 GAGCTC GAGCT^C
1069 GAGCTC
SapI 939 GCTCTTC GCTCTTC(1/4)
1047 GCTCTTC
TaqI 479 TCGA T^CGA
1202 TCGA
Tsp509I 281 AATT ^AATT
1360 AATT
TthHB8I 479 TCGA T^CGA
1202 TCGA
XhoII 371 AGATCT [GA]^GATC[CT]
1460 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BalI BanI BanII BbsI BbvI
BmyI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrBI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI SmlI StyI TseI Tsp45I
TspRI XcmI