################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvrII BamHI BbeI BbvCI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI HaeII HindIII HpaI KasI KpnI KspI MamI MfeI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 214 GACGTC GACGT^C AccI 1117 GTCGAC GT^[AC][GT]AC AccIII 250 TCCGGA T^CCGGA AcsI 1462 GAATTC [GA]^AATT[CT] ApoI 1462 GAATTC [GA]^AATT[CT] AviII 830 TGCGCA TGC^GCA BalI 470 TGGCCA TGG^CCA BclI 1091 TGATCA T^GATCA BfaI 563 CTAG C^TAG BpmI 107 CTCCAG CTGGAG(16/14) BsaWI 250 TCCGGA [AT]^CCGG[AT] BseAI 250 TCCGGA T^CCGGA BsmBI 719 CGTCTC CGTCTC(1/5) BspEI 250 TCCGGA T^CCGGA BsrFI 619 GCCGGC [GA]^CCGG[CT] BstAPI 1410 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 619 GCCGGC [GA]^CCGG[CT] DraI 1011 TTTAAA TTT^AAA EcoRI 1462 GAATTC G^AATTC FbaI 1091 TGATCA T^GATCA FspI 830 TGCGCA TGC^GCA HincII 1117 GTCGAC GT[CT]^[GA]AC HindII 1117 GTCGAC GT[CT]^[GA]AC MaeI 563 CTAG C^TAG MluI 716 ACGCGT A^CGCGT MluNI 470 TGGCCA TGG^CCA MroI 250 TCCGGA T^CCGGA MscI 470 TGGCCA TGG^CCA MspA1I 724 CAGCTG C[AC]G^C[GT]G NaeI 619 GCCGGC GCC^GGC NgoMIV 619 GCCGGC G^CCGGC PpuMI 25 GGGTCCT [GA]G^G[AT]CC[CT] PstI 703 CTGCAG CTGCA^G PvuII 724 CAGCTG CAG^CTG SalI 1117 GTCGAC G^TCGAC SapI 1047 GAAGAGC GCTCTTC(1/4) ScaI 947 AGTACT AGT^ACT ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 214 GACGTC G[GA]^CG[CT]C 384 GACGCC AflIII 716 ACGCGT A^C[GA][CT]GT 890 ACGTGT AlwI 1269 GATCC GGATC(4/5) 439 GGATC ApaI 586 GGGCCC GGGCC^C 1109 GGGCCC Asp700I 277 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 991 GAAGTGGTTC BbrPI 631 CACGTG CAC^GTG 1612 CACGTG BbsI 277 GAAGAC GAAGAC(2/6) 1735 GAAGAC BcgI 1494 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1419 GCAATGCGTTCG BpuAI 277 GAAGAC GAAGAC(2/6) 1735 GAAGAC BsaHI 214 GACGTC G[GA]^CG[CT]C 384 GACGCC BsgI 592 CTGCAC GTGCAG(16/14) 409 GTGCAG BstF5I 1248 CATCC GGATG(2/0) 980 GGATG BstYI 439 GGATCT [GA]^GATC[CT] 1268 AGATCC DraIII 628 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 631 CACGTGGTG DsaI 686 CCATGG C^C[GA][CT]GG 1238 CCATGG EaeI 470 TGGCCA [CT]^GGCC[GA] 711 CGGCCA EcoT14I 686 CCATGG C^C[AT][AT]GG 1238 CCATGG FokI 1248 CATCC GGATG(9/13) 980 GGATG Hin1I 214 GACGTC G[GA]^CG[CT]C 384 GACGCC MflI 439 GGATCT [GA]^GATC[CT] 1268 AGATCC NcoI 686 CCATGG C^CATGG 1238 CCATGG PflMI 627 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 630 CCACGTGGTGG PleI 1120 GACTC GAGTC(4/5) 1086 GAGTC PmaCI 631 CACGTG CAC^GTG 1612 CACGTG PmlI 631 CACGTG CAC^GTG 1612 CACGTG PshAI 254 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 922 GACAAGCGTC SmlI 35 CTCAAG C^T[CT][GA]AG 1552 CTCAAG StyI 686 CCATGG C^C[AT][AT]GG 1238 CCATGG Tsp45I 1534 GTCAC ^GT[GC]AC 1615 GTGAC Van91I 627 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 630 CCACGTGGTGG XhoII 439 GGATCT [GA]^GATC[CT] 1268 AGATCC XmnI 277 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 991 GAAGTGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AfaI AluI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XmaCI XmaI