Restriction map for
pF1KE2354
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# NO CUT ENZYMES ( 71 enzymes) #
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AclI AgeI AhdI Alw44I Aor51HI
ApaLI AscI AspEI AspI AvaI
AviII AvrII BbeI BbrPI BglI
BlnI BsaAI BsiWI BsoBI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
CpoI DrdI Eam1105I Eco47III EcoNI
EcoO65I FseI FspI HaeII HpaI
KasI KspI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SspI StuI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 490 GACGTC GACGT^C
Acc65I 8358 GGTACC G^GTACC
AccIII 4704 TCCGGA T^CCGGA
Asp718I 8358 GGTACC G^GTACC
BbvCI 6139 GCTGAGG CCTCAGC(-5/-2)
BcgI 1266 CGATGTTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 3031 CCGGG CC^[GC]GG
BlpI 7924 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 7924 GCTGAGC GC^T[ACGT]AGC
BseAI 4704 TCCGGA T^CCGGA
BsiEI 6608 CGGCCG CG[GA][CT]^CG
BspDI 4217 ATCGAT AT^CGAT
BspEI 4704 TCCGGA T^CCGGA
BstBI 8029 TTCGAA TT^CGAA
CelII 7924 GCTGAGC GC^T[ACGT]AGC
ClaI 4217 ATCGAT AT^CGAT
DraIII 7385 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 6608 CGGCCG C^GGCCG
EclXI 6608 CGGCCG C^GGCCG
Eco52I 6608 CGGCCG C^GGCCG
EcoT22I 871 ATGCAT ATGCA^T
KpnI 8358 GGTACC GGTAC^C
MroI 4704 TCCGGA T^CCGGA
NciI 3031 CCGGG CC^[GC]GG
NdeI 144 CATATG CA^TATG
NsiI 871 ATGCAT ATGCA^T
NspV 8029 TTCGAA TT^CGAA
PpuMI 2277 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 68 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 8927 ACCAGGT A^CC[AT]GGT
SfuI 8029 TTCGAA TT^CGAA
SpeI 7678 ACTAGT A^CTAGT
XbaI 414 TCTAGA T^CTAGA
XcmI 1692 CCAGGAAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AcyI 490 GACGTC G[GA]^CG[CT]C
3942 GGCGTC
AflII 631 CTTAAG C^TTAAG
1080 CTTAAG
AocI 1183 CCTAAGG CC^T[ACGT]AGG
3862 CCTGAGG
ApaI 2697 GGGCCC GGGCC^C
6655 GGGCCC
AseI 157 ATTAAT AT^TAAT
996 ATTAAT
AsnI 157 ATTAAT AT^TAAT
996 ATTAAT
BamHI 174 GGATCC G^GATCC
8909 GGATCC
BanI 6613 GGTGCC G^G[CT][GA]CC
8358 GGTACC
BclI 1779 TGATCA T^GATCA
6558 TGATCA
BfrI 631 CTTAAG C^TTAAG
1080 CTTAAG
BsaBI 2176 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2557 GATGGATATC
BsaHI 490 GACGTC G[GA]^CG[CT]C
3942 GGCGTC
BsgI 43 GTGCAG GTGCAG(16/14)
754 GTGCAG
BsmBI 2339 CGTCTC CGTCTC(1/5)
3702 GAGACG
BspHI 3459 TCATGA T^CATGA
3984 TCATGA
BsrFI 6610 GCCGGT [GA]^CCGG[CT]
8668 GCCGGT
Bsu36I 1183 CCTAAGG CC^T[ACGT]AGG
3862 CCTGAGG
Cfr10I 6610 GCCGGT [GA]^CCGG[CT]
8668 GCCGGT
DraII 2277 AGGTCCT [GA]G^G[ACGT]CC[CT]
2696 AGGGCCC
Eco81I 1183 CCTAAGG CC^T[ACGT]AGG
3862 CCTGAGG
EcoO109I 2277 AGGTCCT [GA]G^G[ACGT]CC[CT]
2696 AGGGCCC
FauI 1139 GCGGG CCCGC(4/6)
6114 GCGGG
FbaI 1779 TGATCA T^GATCA
6558 TGATCA
HgaI 1945 GACGC GACGC(5/10)
3943 GCGTC
Hin1I 490 GACGTC G[GA]^CG[CT]C
3942 GGCGTC
HindIII 4361 AAGCTT A^AGCTT
5543 AAGCTT
MamI 2176 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2557 GATGGATATC
PshBI 157 ATTAAT AT^TAAT
996 ATTAAT
RcaI 3459 TCATGA T^CATGA
3984 TCATGA
SapI 335 GAAGAGC GCTCTTC(1/4)
793 GCTCTTC
ScaI 6237 AGTACT AGT^ACT
6923 AGTACT
SphI 873 GCATGC GCATG^C
3798 GCATGC
SwaI 4368 ATTTAAAT ATTT^AAAT
5691 ATTTAAAT
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# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BalI BanII
BbsI BbvI BciVI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRI EcoRII EcoRV
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI