Restriction map for
pF1KE2332
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspHI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssKI BssSI
BstBI BstEII BstNI BstPI BstUI
BstXI Cac8I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRII
EcoRV EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MluI
MroI MspA1I MspI MvaI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 768 GTATAC GT^[AC][GT]AC
AflII 1243 CTTAAG C^TTAAG
AhdI 523 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 210 AGCT AG^CT
AocI 1567 CCTGAGG CC^T[ACGT]AGG
AspEI 523 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 834 TGGCCA TGG^CCA
BanI 729 GGCACC G^G[CT][GA]CC
BanII 1365 GAGCCC G[GA]GC[CT]^C
BbvI 199 GCAGC GCAGC(8/12)
BclI 945 TGATCA T^GATCA
BfaI 1156 CTAG C^TAG
BfrI 1243 CTTAAG C^TTAAG
BmyI 1365 GAGCCC G[AGT]GC[ACT]^C
BsaAI 552 TACGTG [CT]AC^GT[GA]
BsiYI 91 CCTAATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 91 CCTAATTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 1365 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 1648 ACATGT A^CATGT
BspMI 19 GCAGGT ACCTGC(4/8)
BsrI 1007 CCAGT ACTGG(1/-1)
Bst1107I 768 GTATAC GTA^TAC
BstAPI 187 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 768 GTATAC GTA^TAC
Bsu36I 1567 CCTGAGG CC^T[ACGT]AGG
DraI 799 TTTAAA TTT^AAA
DsaI 290 CCATGG C^C[GA][CT]GG
Eam1105I 523 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1567 CCTGAGG CC^T[ACGT]AGG
EcoNI 91 CCTAATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 945 TGATCA T^GATCA
MaeI 1156 CTAG C^TAG
MfeI 463 CAATTG C^AATTG
MluNI 834 TGGCCA TGG^CCA
MscI 834 TGGCCA TGG^CCA
MunI 463 CAATTG C^AATTG
NcoI 290 CCATGG C^CATGG
NspI 1648 ACATGT [GA]CATG^[CT]
PleI 559 GAGTC GAGTC(4/5)
ScaI 69 AGTACT AGT^ACT
SfcI 1543 CTACAG C^T[GA][CT]AG
SspI 955 AATATT AAT^ATT
StuI 306 AGGCCT AGG^CCT
TaqI 1575 TCGA T^CGA
TseI 199 GCAGC G^C[AT]GC
TthHB8I 1575 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 70 GTAC GT^AC
434 GTAC
AseI 813 ATTAAT AT^TAAT
1227 ATTAAT
AsnI 813 ATTAAT AT^TAAT
1227 ATTAAT
Asp700I 385 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1438 GAACTTCTTC
BglII 721 AGATCT A^GATCT
1239 AGATCT
BsaBI 991 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1033 GATTATGATC
BsaI 1003 GAGACC GGTCTC(1/5)
691 GGTCTC
BsaJI 290 CCATGG C^C[ACGT][ACGT]GG
1118 CCTTGG
BstYI 721 AGATCT [GA]^GATC[CT]
1239 AGATCT
EaeI 467 TGGCCG [CT]^GGCC[GA]
834 TGGCCA
EcoRI 270 GAATTC G^AATTC
1099 GAATTC
EcoT14I 290 CCATGG C^C[AT][AT]GG
1118 CCTTGG
MamI 991 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1033 GATTATGATC
MflI 721 AGATCT [GA]^GATC[CT]
1239 AGATCT
NlaIV 729 GGCACC GG[ACGT]^[ACGT]CC
1114 GGGACC
PshBI 813 ATTAAT AT^TAAT
1227 ATTAAT
RsaI 70 GTAC GT^AC
434 GTAC
SfaNI 364 GCATC GCATC(5/9)
1502 GCATC
SmlI 1021 CTTGAG C^T[CT][GA]AG
1243 CTTAAG
StyI 290 CCATGG C^C[AT][AT]GG
1118 CCTTGG
TfiI 601 GAATC G^A[AT]TC
1015 GATTC
XhoII 721 AGATCT [GA]^GATC[CT]
1239 AGATCT
XmnI 385 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1438 GAACTTCTTC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AflIII AlwI ApoI
AvaII BsmAI BsmFI BstF5I Cfr13I
DdeI DpnII DraII EarI EcoO109I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MslI MwoI
NdeII NlaIII PpuMI Sau3AI Sau96I
Tru9I Tsp45I Tsp509I TspRI