Restriction map for
pF1KE2521
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BbeI BbrPI BbvCI
BclI BcnI BfrI BglI BlnI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CfoI ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeII MamI
MluI MluNI MroI MscI MslI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AgeI 1233 ACCGGT A^CCGGT
AocI 246 CCTCAGG CC^T[ACGT]AGG
AvaII 838 GGACC G^G[AT]CC
BamHI 1596 GGATCC G^GATCC
BanII 1107 GAGCCC G[GA]GC[CT]^C
BcgI 1061 CGAATGTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 242 GTATCC GTATCC(6/5)
BmyI 1107 GAGCCC G[AGT]GC[ACT]^C
BsaWI 1233 ACCGGT [AT]^CCGG[AT]
BsgI 674 GTGCAG GTGCAG(16/14)
BsmAI 1117 GAGAC GTCTC(1/5)
Bsp1286I 1107 GAGCCC G[AGT]GC[ACT]^C
BsrBI 340 GAGCGG CCGCTC(-3/-3)
BsrDI 917 GCAATG GCAATG(2/0)
BsrFI 1233 ACCGGT [GA]^CCGG[CT]
BstEII 828 GGTGACC G^GT[ACGT]ACC
BstPI 828 GGTGACC G^GT[ACGT]ACC
Bsu36I 246 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1233 ACCGGT [GA]^CCGG[CT]
Cfr13I 838 GGACC G^G[ACGT]CC
DsaI 524 CCATGG C^C[GA][CT]GG
Eco81I 246 CCTCAGG CC^T[ACGT]AGG
EcoNI 794 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 828 GGTGACC G^GT[ACGT]ACC
EcoRI 1146 GAATTC G^AATTC
FauI 1026 CCCGC CCCGC(4/6)
HapII 1234 CCGG C^CGG
HgaI 81 GCGTC GACGC(5/10)
HpaII 1234 CCGG C^CGG
MspA1I 168 CAGCGG C[AC]G^C[GT]G
MspI 1234 CCGG C^CGG
NcoI 524 CCATGG C^CATGG
PinAI 1233 ACCGGT A^CCGGT
Sau96I 838 GGACC G^G[ACGT]CC
SspI 1122 AATATT AAT^ATT
TaqI 1060 TCGA T^CGA
TthHB8I 1060 TCGA T^CGA
XcmI 902 CCATACACAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 1146 GAATTC [GA]^AATT[CT]
1503 AAATTC
ApoI 1146 GAATTC [GA]^AATT[CT]
1503 AAATTC
BanI 688 GGCACC G^G[CT][GA]CC
702 GGCACC
BglII 270 AGATCT A^GATCT
364 AGATCT
BsmFI 1588 GGGAC GGGAC(10/14)
962 GTCCC
BspHI 771 TCATGA T^CATGA
1470 TCATGA
Cac8I 1263 GCAGGC GC[ACGT]^[ACGT]GC
1422 GCCAGC
DraI 318 TTTAAA TTT^AAA
604 TTTAAA
MaeIII 829 GTGAC ^GT[ACGT]AC
1237 GTGAC
MfeI 335 CAATTG C^AATTG
404 CAATTG
MunI 335 CAATTG C^AATTG
404 CAATTG
RcaI 771 TCATGA T^CATGA
1470 TCATGA
SfaNI 1081 GATGC GCATC(5/9)
1567 GATGC
SmlI 135 CTTGAG C^T[CT][GA]AG
253 CTTGAG
StuI 131 AGGCCT AGG^CCT
802 AGGCCT
Tsp45I 829 GTGAC ^GT[GC]AC
1237 GTGAC
XbaI 153 TCTAGA T^CTAGA
231 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI BbsI
BbvI BfaI BlpI BpmI Bpu1102I
BpuAI BsaJI BseRI BsiYI BslI
BsrI BssKI BstF5I BstNI BstYI
CelII DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI ScrFI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII