Restriction map for
pF1KE3328
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AvaI
AviII AvrII BanI BbeI BbvCI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
PacI PaeR7I PflMI PinAI PmeI
PshAI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 21 TCCGGA T^CCGGA
AclI 1772 AACGTT AA^CGTT
AflIII 1453 ACATGT A^C[GA][CT]GT
Alw44I 2217 GTGCAC G^TGCAC
AocI 2200 CCTGAGG CC^T[ACGT]AGG
ApaLI 2217 GTGCAC G^TGCAC
BalI 735 TGGCCA TGG^CCA
BamHI 228 GGATCC G^GATCC
BbrPI 95 CACGTG CAC^GTG
BcgI 2214 GCAGTGCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 2556 CCGGG CC^[GC]GG
BsaAI 95 CACGTG [CT]AC^GT[GA]
BsaBI 1396 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 21 TCCGGA [AT]^CCGG[AT]
BseAI 21 TCCGGA T^CCGGA
BsmI 987 GAATGC GAATGC(1/-1)
BspEI 21 TCCGGA T^CCGGA
BspLU11I 1453 ACATGT A^CATGT
BsrDI 812 CATTGC GCAATG(2/0)
BstYI 228 GGATCC [GA]^GATC[CT]
Bsu36I 2200 CCTGAGG CC^T[ACGT]AGG
DraI 480 TTTAAA TTT^AAA
DsaI 1350 CCACGG C^C[GA][CT]GG
Eco81I 2200 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2761 ATGCAT ATGCA^T
MamI 1396 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 228 GGATCC [GA]^GATC[CT]
MluNI 735 TGGCCA TGG^CCA
MroI 21 TCCGGA T^CCGGA
MscI 735 TGGCCA TGG^CCA
MslI 337 CACTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 2556 CCGGG CC^[GC]GG
NdeI 371 CATATG CA^TATG
NsiI 2761 ATGCAT ATGCA^T
PleI 2574 GAGTC GAGTC(4/5)
PmaCI 95 CACGTG CAC^GTG
PmlI 95 CACGTG CAC^GTG
Psp1406I 1772 AACGTT AA^CGTT
PstI 406 CTGCAG CTGCA^G
SacI 2754 GAGCTC GAGCT^C
SwaI 479 ATTTAAAT ATTT^AAAT
XhoII 228 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AseI 330 ATTAAT AT^TAAT
1992 ATTAAT
AsnI 330 ATTAAT AT^TAAT
1992 ATTAAT
AspHI 2217 GTGCAC G[AT]GC[AT]^C
2754 GAGCTC
AspI 1006 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1261 GACCTAGTC
BanII 574 GGGCTC G[GA]GC[CT]^C
2754 GAGCTC
BciVI 694 GGATAC GTATCC(6/5)
2740 GTATCC
BclI 650 TGATCA T^GATCA
1281 TGATCA
BpmI 236 CTGGAG CTGGAG(16/14)
1559 CTGGAG
BsaI 1518 GAGACC GGTCTC(1/5)
2516 GGTCTC
BsiHKAI 2217 GTGCAC G[AT]GC[AT]^C
2754 GAGCTC
BspHI 1956 TCATGA T^CATGA
2450 TCATGA
BstBI 554 TTCGAA TT^CGAA
2602 TTCGAA
BstXI 2015 CCAAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2649 CCAAAGTGCTGG
DraIII 266 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2211 CACGCAGTG
EaeI 735 TGGCCA [CT]^GGCC[GA]
2568 TGGCCG
EarI 1965 GAAGAG CTCTTC(1/4)
2461 GAAGAG
EcoRI 103 GAATTC G^AATTC
312 GAATTC
FbaI 650 TGATCA T^GATCA
1281 TGATCA
Ksp632I 1965 GAAGAG CTCTTC(1/4)
2461 GAAGAG
NspV 554 TTCGAA TT^CGAA
2602 TTCGAA
PflFI 1006 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1261 GACCTAGTC
PshBI 330 ATTAAT AT^TAAT
1992 ATTAAT
PvuII 5 CAGCTG CAG^CTG
166 CAGCTG
RcaI 1956 TCATGA T^CATGA
2450 TCATGA
SfcI 406 CTGCAG C^T[GA][CT]AG
2140 CTACAG
SfuI 554 TTCGAA TT^CGAA
2602 TTCGAA
SphI 1132 GCATGC GCATG^C
2103 GCATGC
Tth111I 1006 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1261 GACCTAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI