Restriction map for
pF1KE1599
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AspHI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BstUI Bsu36I
CelII Cfr10I ClaI CpoI DsaI
EagI EclXI Eco47III Eco52I Eco81I
FauI FseI FspI HaeII HapII
HgaI Hin1I HindIII HpaII KasI
KspI MluI MroI MspI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PstI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 786 GGTACC G^GTACC
AciI 1372 GCGG CCGC(-3/-1)
AclI 2679 AACGTT AA^CGTT
AhdI 2718 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 786 GGTACC G^GTACC
AspEI 2718 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1202 CTCGAG C^[CT]CG[GA]G
BalI 2144 TGGCCA TGG^CCA
BanI 786 GGTACC G^G[CT][GA]CC
BanII 1450 GAGCCC G[GA]GC[CT]^C
BbvCI 2899 GCTGAGG CCTCAGC(-5/-2)
BciVI 2273 GGATAC GTATCC(6/5)
BglII 2838 AGATCT A^GATCT
BsaI 2817 GGTCTC GGTCTC(1/5)
BseRI 971 CTCCTC GAGGAG(10/8)
BsoBI 1202 CTCGAG C^[CT]CG[GA]G
BspHI 570 TCATGA T^CATGA
BspMI 2518 GCAGGT ACCTGC(4/8)
BssSI 974 CTCGTG CACGAG(-5/-1)
Bst1107I 890 GTATAC GTA^TAC
BstAPI 1813 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 177 TTCGAA TT^CGAA
BstYI 2838 AGATCT [GA]^GATC[CT]
BstZ17I 890 GTATAC GTA^TAC
CfoI 1833 GCGC GCG^C
DraII 1923 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 2144 TGGCCA [CT]^GGCC[GA]
Eam1105I 2718 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1915 CCTATGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1923 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 825 GAATTC G^AATTC
EcoRV 2761 GATATC GAT^ATC
EcoT14I 88 CCTTGG C^C[AT][AT]GG
EcoT22I 1491 ATGCAT ATGCA^T
HhaI 1833 GCGC GCG^C
HinP1I 1833 GCGC G^CGC
HpaI 2884 GTTAAC GTT^AAC
KpnI 786 GGTACC GGTAC^C
MaeII 2680 ACGT A^CGT
MflI 2838 AGATCT [GA]^GATC[CT]
MluNI 2144 TGGCCA TGG^CCA
MscI 2144 TGGCCA TGG^CCA
MspA1I 197 CAGCTG C[AC]G^C[GT]G
NdeI 730 CATATG CA^TATG
NsiI 1491 ATGCAT ATGCA^T
NspV 177 TTCGAA TT^CGAA
PaeR7I 1202 CTCGAG C^TCGAG
PpuMI 1923 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2679 AACGTT AA^CGTT
PvuII 197 CAGCTG CAG^CTG
RcaI 570 TCATGA T^CATGA
ScaI 967 AGTACT AGT^ACT
SfuI 177 TTCGAA TT^CGAA
SphI 110 GCATGC GCATG^C
StyI 88 CCTTGG C^C[AT][AT]GG
XhoI 1202 CTCGAG C^TCGAG
XhoII 2838 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 883 GTAGAC GT^[AC][GT]AC
890 GTATAC
AflIII 1579 ACATGT A^C[GA][CT]GT
2719 ACATGT
AlwI 712 GATCC GGATC(4/5)
1541 GGATC
BbsI 3405 GAAGAC GAAGAC(2/6)
903 GTCTTC
BbvI 1406 GCAGC GCAGC(8/12)
1235 GCTGC
BpuAI 3405 GAAGAC GAAGAC(2/6)
903 GTCTTC
BsaBI 1536 GATTAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3121 GATCAGCATC
BsmI 1249 GAATGC GAATGC(1/-1)
1489 GAATGC
BspLU11I 1579 ACATGT A^CATGT
2719 ACATGT
BsrDI 1238 GCAATG GCAATG(2/0)
2060 GCAATG
BstEII 2913 GGTAACC G^GT[ACGT]ACC
3243 GGTCACC
BstPI 2913 GGTAACC G^GT[ACGT]ACC
3243 GGTCACC
BstXI 692 CCATAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
946 CCAGGAATCTGG
DraI 756 TTTAAA TTT^AAA
2244 TTTAAA
DrdI 853 GACAAGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3282 GACTGACAAGTC
EcoO65I 2913 GGTAACC G^GT[ACGT]ACC
3243 GGTCACC
Fnu4HI 1235 GCTGC GC^[ACGT]GC
1406 GCAGC
HincII 1347 GTCAAC GT[CT]^[GA]AC
2884 GTTAAC
HindII 1347 GTCAAC GT[CT]^[GA]AC
2884 GTTAAC
ItaI 1235 GCTGC GC^[ACGT]GC
1406 GCAGC
MamI 1536 GATTAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3121 GATCAGCATC
SmlI 1202 CTCGAG C^T[CT][GA]AG
2486 CTCAAG
SspI 815 AATATT AAT^ATT
2439 AATATT
TaqI 178 TCGA T^CGA
1203 TCGA
TseI 1235 GCTGC G^C[AT]GC
1406 GCAGC
TthHB8I 178 TCGA T^CGA
1203 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI Asp700I AvaII BclI BfaI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraIII EarI EcoRII FbaI
FokI HaeIII HinfI HphI Ksp632I
MaeI MaeIII MboI MboII MfeI
MnlI MseI MslI MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I Tsp45I
Tsp509I TspRI XcmI XmnI