Restriction map for
pF1KE2429
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BcgI BclI BfrI BglII
BlnI BsaAI BsaBI BsaHI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
ClaI CpoI DraIII Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI MaeII MamI
MfeI MflI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI XbaI XhoII
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 1937 ACATGT A^C[GA][CT]GT
Alw44I 197 GTGCAC G^TGCAC
ApaLI 197 GTGCAC G^TGCAC
BanI 3 GGTGCC G^G[CT][GA]CC
BbvCI 72 CCTCAGC CCTCAGC(-5/-2)
BciVI 627 GTATCC GTATCC(6/5)
BfaI 979 CTAG C^TAG
BlpI 1380 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1380 GCTGAGC GC^T[ACGT]AGC
BsaWI 479 TCCGGT [AT]^CCGG[AT]
BsgI 1234 GTGCAG GTGCAG(16/14)
BsiEI 1488 CGGCCG CG[GA][CT]^CG
BspHI 1848 TCATGA T^CATGA
BspLU11I 1937 ACATGT A^CATGT
BspMI 101 ACCTGC ACCTGC(4/8)
BsrFI 79 GCCGGC [GA]^CCGG[CT]
BssHII 21 GCGCGC G^CGCGC
BssSI 1764 CACGAG CACGAG(-5/-1)
CelII 1380 GCTGAGC GC^T[ACGT]AGC
Cfr10I 79 GCCGGC [GA]^CCGG[CT]
DraI 1162 TTTAAA TTT^AAA
DrdI 1219 GACTCTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1488 CGGCCG C^GGCCG
EclXI 1488 CGGCCG C^GGCCG
Eco52I 1488 CGGCCG C^GGCCG
EcoNI 1544 CCTTTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1360 GAATTC G^AATTC
HaeII 76 AGCGCC [GA]GCGC^[CT]
KspI 41 CCGCGG CCGC^GG
MaeI 979 CTAG C^TAG
MslI 1030 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 79 GCCGGC GCC^GGC
NcoI 1614 CCATGG C^CATGG
NgoMIV 79 GCCGGC G^CCGGC
NspI 1937 ACATGT [GA]CATG^[CT]
PaeR7I 368 CTCGAG C^TCGAG
PflMI 1911 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 103 CTGCAG CTGCA^G
RcaI 1848 TCATGA T^CATGA
SacII 41 CCGCGG CCGC^GG
SexAI 614 ACCTGGT A^CC[AT]GGT
SmlI 368 CTCGAG C^T[CT][GA]AG
Van91I 1911 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 719 CCAGACACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 368 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccII 22 CGCG CG^CG
42 CGCG
AcsI 747 AAATTT [GA]^AATT[CT]
1360 GAATTC
AlwI 771 GGATC GGATC(4/5)
1700 GGATC
AlwNI 97 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
910 CAGAAGCTG
AocI 735 CCTAAGG CC^T[ACGT]AGG
942 CCTGAGG
ApaI 36 GGGCCC GGGCC^C
88 GGGCCC
ApoI 747 AAATTT [GA]^AATT[CT]
1360 GAATTC
AspI 1000 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1330 GACTGCGTC
BglI 1399 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1557 GCCCTCCAGGC
BpmI 1560 CTCCAG CTGGAG(16/14)
511 CTGGAG
BsaI 1498 GAGACC GGTCTC(1/5)
1815 GGTCTC
BseRI 934 GAGGAG GAGGAG(10/8)
1349 GAGGAG
BsmI 1408 GCATTC GAATGC(1/-1)
1477 GCATTC
BstUI 22 CGCG CG^CG
42 CGCG
Bsu36I 735 CCTAAGG CC^T[ACGT]AGG
942 CCTGAGG
DpnII 772 GATC ^GATC
1701 GATC
DsaI 41 CCGCGG C^C[GA][CT]GG
1614 CCATGG
EarI 700 CTCTTC CTCTTC(1/4)
1875 CTCTTC
Eco81I 735 CCTAAGG CC^T[ACGT]AGG
942 CCTGAGG
HgaI 1390 GACGC GACGC(5/10)
1334 GCGTC
Ksp632I 700 CTCTTC CTCTTC(1/4)
1875 CTCTTC
MboI 772 GATC ^GATC
1701 GATC
MvnI 22 CGCG CG^CG
42 CGCG
NdeII 772 GATC ^GATC
1701 GATC
PflFI 1000 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1330 GACTGCGTC
PpuMI 339 GGGACCC [GA]G^G[AT]CC[CT]
998 AGGACCT
SacI 901 GAGCTC GAGCT^C
1714 GAGCTC
Sau3AI 772 GATC ^GATC
1701 GATC
SfcI 103 CTGCAG C^T[GA][CT]AG
1464 CTGTAG
StuI 1541 AGGCCT AGG^CCT
1925 AGGCCT
TaqI 369 TCGA T^CGA
1250 TCGA
Tth111I 1000 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1330 GACTGCGTC
TthHB8I 369 TCGA T^CGA
1250 TCGA
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BalI BanII BbsI BbvI
BcnI BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PleI
PvuII RsaI Sau96I ScrFI SfaNI
SmaI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XmaCI XmaI