Restriction map for
pF1KE2654
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwNI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 190 GTCTAC GT^[AC][GT]AC
AciI 777 GCGG CCGC(-3/-1)
Alw44I 788 GTGCAC G^TGCAC
AocI 304 CCTGAGG CC^T[ACGT]AGG
ApaLI 788 GTGCAC G^TGCAC
Asp700I 159 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 477 CCCGGG C^[CT]CG[GA]G
AvaII 77 GGTCC G^G[AT]CC
BamHI 53 GGATCC G^GATCC
BanI 718 GGCACC G^G[CT][GA]CC
BbsI 424 GTCTTC GAAGAC(2/6)
BbvI 780 GCTGC GCAGC(8/12)
BlpI 255 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 255 GCTGAGC GC^T[ACGT]AGC
BpuAI 424 GTCTTC GAAGAC(2/6)
BsaAI 112 TACGTG [CT]AC^GT[GA]
BsaI 633 GGTCTC GGTCTC(1/5)
BsgI 640 CTGCAC GTGCAG(16/14)
BsmAI 634 GTCTC GTCTC(1/5)
BsmFI 78 GTCCC GGGAC(10/14)
BsoBI 477 CCCGGG C^[CT]CG[GA]G
BspHI 182 TCATGA T^CATGA
BspLU11I 420 ACATGT A^CATGT
BspMI 405 GCAGGT ACCTGC(4/8)
BstEII 408 GGTGACC G^GT[ACGT]ACC
BstPI 408 GGTGACC G^GT[ACGT]ACC
BstXI 168 CCAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 304 CCTGAGG CC^T[ACGT]AGG
CelII 255 GCTGAGC GC^T[ACGT]AGC
CfoI 273 GCGC GCG^C
DraII 76 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 433 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 174 CCGTGG C^C[GA][CT]GG
Eco81I 304 CCTGAGG CC^T[ACGT]AGG
EcoO109I 76 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 408 GGTGACC G^GT[ACGT]ACC
HaeII 272 GGCGCT [GA]GCGC^[CT]
HhaI 273 GCGC GCG^C
HinP1I 273 GCGC G^CGC
MaeII 113 ACGT A^CGT
MslI 705 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 743 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 76 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 182 TCATGA T^CATGA
SacI 806 GAGCTC GAGCT^C
SapI 336 GCTCTTC GCTCTTC(1/4)
ScaI 653 AGTACT AGT^ACT
SfaNI 143 GCATC GCATC(5/9)
SmaI 477 CCCGGG CCC^GGG
TseI 780 GCTGC G^C[AT]GC
Van91I 743 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 477 CCCGGG C^CCGGG
XmaI 477 CCCGGG C^CCGGG
XmnI 159 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 113 ACGTGT A^C[GA][CT]GT
420 ACATGT
BanII 489 GGGCTC G[GA]GC[CT]^C
806 GAGCTC
BpmI 809 CTCCAG CTGGAG(16/14)
447 CTGGAG
BstF5I 317 GGATG GGATG(2/0)
710 GGATG
BstYI 45 AGATCC [GA]^GATC[CT]
53 GGATCC
EcoT14I 767 CCAAGG C^C[AT][AT]GG
821 CCAAGG
Fnu4HI 777 GCGGC GC^[ACGT]GC
780 GCTGC
FokI 317 GGATG GGATG(9/13)
710 GGATG
HapII 51 CCGG C^CGG
478 CCGG
HpaII 51 CCGG C^CGG
478 CCGG
ItaI 777 GCGGC GC^[ACGT]GC
780 GCTGC
MflI 45 AGATCC [GA]^GATC[CT]
53 GGATCC
MspA1I 613 CAGCTG C[AC]G^C[GT]G
669 CAGCTG
MspI 51 CCGG C^CGG
478 CCGG
MwoI 162 GCCCTTCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
251 GCTGGCTGAGC
NspI 420 ACATGT [GA]CATG^[CT]
522 GCATGT
PvuII 613 CAGCTG CAG^CTG
669 CAGCTG
StyI 767 CCAAGG C^C[AT][AT]GG
821 CCAAGG
Tsp45I 278 GTGAC ^GT[GC]AC
409 GTGAC
XhoII 45 AGATCC [GA]^GATC[CT]
53 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI AlwI AspHI BcnI
BmyI BsaJI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
HaeIII HphI Ksp632I MaeIII MboI
MboII MnlI MvaI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TspRI