Restriction map for
pF1KE2493
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbeI BbrPI BcgI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MseI MslI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 503 TCCGGA T^CCGGA
AfaI 354 GTAC GT^AC
Alw44I 594 GTGCAC G^TGCAC
AlwNI 779 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 619 CCTGAGG CC^T[ACGT]AGG
ApaI 469 GGGCCC GGGCC^C
ApaLI 594 GTGCAC G^TGCAC
AvaI 388 CCCGAG C^[CT]CG[GA]G
AvaII 555 GGACC G^G[AT]CC
AviII 435 TGCGCA TGC^GCA
BalI 680 TGGCCA TGG^CCA
BanI 412 GGCACC G^G[CT][GA]CC
BbsI 719 GAAGAC GAAGAC(2/6)
BbvCI 398 CCTCAGC CCTCAGC(-5/-2)
BciVI 244 GGATAC GTATCC(6/5)
BglI 209 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 190 CTGGAG CTGGAG(16/14)
BpuAI 719 GAAGAC GAAGAC(2/6)
BsaWI 503 TCCGGA [AT]^CCGG[AT]
BseAI 503 TCCGGA T^CCGGA
BsmI 663 GAATGC GAATGC(1/-1)
BsoBI 388 CCCGAG C^[CT]CG[GA]G
BspEI 503 TCCGGA T^CCGGA
BspMI 356 ACCTGC ACCTGC(4/8)
BsrBI 520 GAGCGG CCGCTC(-3/-3)
BsrDI 438 GCAATG GCAATG(2/0)
BsrFI 653 GCCGGC [GA]^CCGG[CT]
Bsu36I 619 CCTGAGG CC^T[ACGT]AGG
CfoI 436 GCGC GCG^C
Cfr10I 653 GCCGGC [GA]^CCGG[CT]
DraIII 333 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 680 TGGCCA [CT]^GGCC[GA]
EarI 630 GAAGAG CTCTTC(1/4)
Eco81I 619 CCTGAGG CC^T[ACGT]AGG
EcoNI 357 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 435 TGCGCA TGC^GCA
HhaI 436 GCGC GCG^C
HinP1I 436 GCGC G^CGC
HincII 148 GTCAAC GT[CT]^[GA]AC
HindII 148 GTCAAC GT[CT]^[GA]AC
Ksp632I 630 GAAGAG CTCTTC(1/4)
MluNI 680 TGGCCA TGG^CCA
MroI 503 TCCGGA T^CCGGA
MscI 680 TGGCCA TGG^CCA
NaeI 653 GCCGGC GCC^GGC
NcoI 416 CCATGG C^CATGG
NgoMIV 653 GCCGGC G^CCGGC
NspI 540 ACATGC [GA]CATG^[CT]
PstI 776 CTGCAG CTGCA^G
PvuII 401 CAGCTG CAG^CTG
RsaI 354 GTAC GT^AC
SacI 133 GAGCTC GAGCT^C
SfaNI 281 GCATC GCATC(5/9)
SfcI 776 CTGCAG C^T[GA][CT]AG
SmlI 160 CTCAAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcyI 217 GGCGTC G[GA]^CG[CT]C
253 GACGCC
AlwI 68 GGATC GGATC(4/5)
92 GGATC
BcnI 63 CCGGG CC^[GC]GG
587 CCCGG
BsaHI 217 GGCGTC G[GA]^CG[CT]C
253 GACGCC
BsaI 583 GAGACC GGTCTC(1/5)
458 GGTCTC
BsmAI 583 GAGAC GTCTC(1/5)
459 GTCTC
BstAPI 281 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
778 GCAGGGCCTGC
BstF5I 183 CATCC GGATG(2/0)
204 CATCC
DsaI 416 CCATGG C^C[GA][CT]GG
463 CCGTGG
EcoT14I 416 CCATGG C^C[AT][AT]GG
578 CCAAGG
FokI 183 CATCC GGATG(9/13)
204 CATCC
Hin1I 217 GGCGTC G[GA]^CG[CT]C
253 GACGCC
HphI 50 TCACC GGTGA(8/7)
169 TCACC
MboII 630 GAAGA GAAGA(8/7)
719 GAAGA
MspA1I 369 CCGCTG C[AC]G^C[GT]G
401 CAGCTG
NciI 63 CCGGG CC^[GC]GG
587 CCCGG
StuI 113 AGGCCT AGG^CCT
308 AGGCCT
StyI 416 CCATGG C^C[AT][AT]GG
578 CCAAGG
XcmI 407 CCAATGGCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
668 CCAACAGGAACCTGG
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AspHI BanII BbvI
BmyI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HgaI HpaII ItaI
MboI MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TaqI TseI TspRI TthHB8I