Restriction map for
pF1KE2691
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AclI AflII AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AvrII BbeI BfaI BfrI
BlnI BsaBI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI DraI Eam1105I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI NspI PacI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 475 GACGTC GACGT^C
AccIII 2003 TCCGGA T^CCGGA
AcsI 743 AAATTC [GA]^AATT[CT]
AfaI 624 GTAC GT^AC
AgeI 1652 ACCGGT A^CCGGT
ApaI 2093 GGGCCC GGGCC^C
ApoI 743 AAATTC [GA]^AATT[CT]
AspHI 1036 GAGCTC G[AT]GC[AT]^C
AviII 296 TGCGCA TGC^GCA
BalI 1614 TGGCCA TGG^CCA
BbvCI 2254 GCTGAGG CCTCAGC(-5/-2)
BcgI 299 GCACCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2369 GGATAC GTATCC(6/5)
BglII 311 AGATCT A^GATCT
BlpI 114 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 114 GCTCAGC GC^T[ACGT]AGC
BsaI 1171 GAGACC GGTCTC(1/5)
BseAI 2003 TCCGGA T^CCGGA
BsiEI 704 CGGCCG CG[GA][CT]^CG
BsiHKAI 1036 GAGCTC G[AT]GC[AT]^C
BspEI 2003 TCCGGA T^CCGGA
BsrDI 1719 CATTGC GCAATG(2/0)
BstAPI 1929 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 137 TTCGAA TT^CGAA
CelII 114 GCTCAGC GC^T[ACGT]AGC
DraIII 588 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2137 GACCTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 704 CGGCCG C^GGCCG
EclXI 704 CGGCCG C^GGCCG
Eco52I 704 CGGCCG C^GGCCG
FspI 296 TGCGCA TGC^GCA
MluNI 1614 TGGCCA TGG^CCA
MroI 2003 TCCGGA T^CCGGA
MscI 1614 TGGCCA TGG^CCA
NaeI 1210 GCCGGC GCC^GGC
NcoI 102 CCATGG C^CATGG
NgoMIV 1210 GCCGGC G^CCGGC
NspV 137 TTCGAA TT^CGAA
PinAI 1652 ACCGGT A^CCGGT
PpuMI 2135 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1233 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 466 CTGCAG CTGCA^G
PvuII 2023 CAGCTG CAG^CTG
RsaI 624 GTAC GT^AC
SacI 1036 GAGCTC GAGCT^C
SfuI 137 TTCGAA TT^CGAA
SmlI 375 CTCAAG C^T[CT][GA]AG
StuI 260 AGGCCT AGG^CCT
Tsp509I 744 AATT ^AATT
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# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 931 GTCTAC GT^[AC][GT]AC
1507 GTAGAC
AflIII 291 ACACGT A^C[GA][CT]GT
852 ACACGT
AocI 1073 CCTCAGG CC^T[ACGT]AGG
1902 CCTGAGG
Asp700I 597 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1413 GAAGCACTTC
AvaI 2090 CTCGGG C^[CT]CG[GA]G
2179 CCCGAG
BamHI 323 GGATCC G^GATCC
2238 GGATCC
BanI 559 GGCACC G^G[CT][GA]CC
2055 GGTGCC
BbrPI 292 CACGTG CAC^GTG
853 CACGTG
BclI 237 TGATCA T^GATCA
434 TGATCA
BglI 531 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
635 GCCTGGTGGGC
BpmI 109 CTCCAG CTGGAG(16/14)
2026 CTGGAG
BsaAI 292 CACGTG [CT]AC^GT[GA]
853 CACGTG
BsgI 763 CTGCAC GTGCAG(16/14)
1480 GTGCAG
BsoBI 2090 CTCGGG C^[CT]CG[GA]G
2179 CCCGAG
BspHI 341 TCATGA T^CATGA
2285 TCATGA
BspMI 680 ACCTGC ACCTGC(4/8)
2138 ACCTGC
BsrFI 1210 GCCGGC [GA]^CCGG[CT]
1652 ACCGGT
BssSI 155 CACGAG CACGAG(-5/-1)
58 CTCGTG
BstEII 198 GGTCACC G^GT[ACGT]ACC
1624 GGTCACC
BstPI 198 GGTCACC G^GT[ACGT]ACC
1624 GGTCACC
BstXI 503 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1754 CCATCGAGCTGG
Bsu36I 1073 CCTCAGG CC^T[ACGT]AGG
1902 CCTGAGG
Cfr10I 1210 GCCGGC [GA]^CCGG[CT]
1652 ACCGGT
Eco81I 1073 CCTCAGG CC^T[ACGT]AGG
1902 CCTGAGG
EcoNI 432 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
825 CCTCTTTGAGG
EcoO65I 198 GGTCACC G^GT[ACGT]ACC
1624 GGTCACC
FbaI 237 TGATCA T^GATCA
434 TGATCA
MseI 221 TTAA T^TAA
1658 TTAA
MspA1I 1930 CAGCGG C[AC]G^C[GT]G
2023 CAGCTG
PflMI 1731 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2064 CCACAAGCTGG
PmaCI 292 CACGTG CAC^GTG
853 CACGTG
PmlI 292 CACGTG CAC^GTG
853 CACGTG
RcaI 341 TCATGA T^CATGA
2285 TCATGA
SexAI 1297 ACCTGGT A^CC[AT]GGT
2192 ACCTGGT
SfcI 466 CTGCAG C^T[GA][CT]AG
1008 CTACAG
Tru9I 221 TTAA T^TAA
1658 TTAA
Van91I 1731 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2064 CCACAAGCTGG
XcmI 1493 CCATTCGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1754 CCATCGAGCTGGTGG
XmnI 597 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1413 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI Aor51HI AspI AvaII BanII
BbsI BbvI BcnI BmyI BpuAI
BsaHI BsaJI BsaWI BseRI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflFI PleI SapI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
Tsp45I TspRI Tth111I TthHB8I XhoII