Restriction map for
pF1KB7666
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BanI BbeI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccIII 13 TCCGGA T^CCGGA
AflIII 374 ACATGT A^C[GA][CT]GT
AhdI 154 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 566 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 712 CCTCAGG CC^T[ACGT]AGG
AspEI 154 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 487 GAGCAC G[AT]GC[AT]^C
BalI 215 TGGCCA TGG^CCA
BanII 241 GAGCCC G[GA]GC[CT]^C
BbrPI 757 CACGTG CAC^GTG
BbsI 162 GTCTTC GAAGAC(2/6)
BbvCI 766 CCTCAGC CCTCAGC(-5/-2)
BglII 107 AGATCT A^GATCT
BpuAI 162 GTCTTC GAAGAC(2/6)
BsaAI 757 CACGTG [CT]AC^GT[GA]
BsaWI 13 TCCGGA [AT]^CCGG[AT]
BseAI 13 TCCGGA T^CCGGA
BseRI 17 GAGGAG GAGGAG(10/8)
BsiHKAI 487 GAGCAC G[AT]GC[AT]^C
BsmI 825 GAATGC GAATGC(1/-1)
BspEI 13 TCCGGA T^CCGGA
BspLU11I 374 ACATGT A^CATGT
BsrI 682 ACTGG ACTGG(1/-1)
BssSI 208 CACGAG CACGAG(-5/-1)
Bsu36I 712 CCTCAGG CC^T[ACGT]AGG
CfoI 53 GCGC GCG^C
DraIII 208 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 154 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 712 CCTCAGG CC^T[ACGT]AGG
EcoRV 574 GATATC GAT^ATC
EcoT14I 167 CCTTGG C^C[AT][AT]GG
FauI 32 GCGGG CCCGC(4/6)
HhaI 53 GCGC GCG^C
HinP1I 53 GCGC G^CGC
HincII 532 GTCAAC GT[CT]^[GA]AC
HindII 532 GTCAAC GT[CT]^[GA]AC
MaeII 758 ACGT A^CGT
MluNI 215 TGGCCA TGG^CCA
MroI 13 TCCGGA T^CCGGA
MscI 215 TGGCCA TGG^CCA
MseI 812 TTAA T^TAA
MspA1I 1100 CAGCTG C[AC]G^C[GT]G
PaeR7I 324 CTCGAG C^TCGAG
PflMI 625 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 178 GAGTC GAGTC(4/5)
PmaCI 757 CACGTG CAC^GTG
PmlI 757 CACGTG CAC^GTG
PvuII 1100 CAGCTG CAG^CTG
SapI 943 GAAGAGC GCTCTTC(1/4)
SfcI 1063 CTACAG C^T[GA][CT]AG
SmlI 324 CTCGAG C^T[CT][GA]AG
SphI 927 GCATGC GCATG^C
SspI 956 AATATT AAT^ATT
StuI 297 AGGCCT AGG^CCT
StyI 167 CCTTGG C^C[AT][AT]GG
TaqI 325 TCGA T^CGA
Tru9I 812 TTAA T^TAA
TthHB8I 325 TCGA T^CGA
Van91I 625 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 625 CCACCTCTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 324 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaI 324 CTCGAG C^[CT]CG[GA]G
405 CCCGAG
BpmI 497 CTCCAG CTGGAG(16/14)
563 CTCCAG
BsgI 433 GTGCAG GTGCAG(16/14)
893 GTGCAG
BsmAI 1022 GAGAC GTCTC(1/5)
597 GTCTC
BsoBI 324 CTCGAG C^[CT]CG[GA]G
405 CCCGAG
BstXI 675 CCACACAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
906 CCAGATTCTTGG
BstYI 107 AGATCT [GA]^GATC[CT]
385 GGATCT
DraII 38 GGGACCC [GA]G^G[ACGT]CC[CT]
312 GGGACCC
DsaI 56 CCACGG C^C[GA][CT]GG
428 CCGTGG
EaeI 194 CGGCCA [CT]^GGCC[GA]
215 TGGCCA
EcoO109I 38 GGGACCC [GA]G^G[ACGT]CC[CT]
312 GGGACCC
MflI 107 AGATCT [GA]^GATC[CT]
385 GGATCT
NdeI 649 CATATG CA^TATG
991 CATATG
PpuMI 38 GGGACCC [GA]G^G[AT]CC[CT]
312 GGGACCC
Tsp509I 965 AATT ^AATT
1027 AATT
XhoII 107 AGATCT [GA]^GATC[CT]
385 GGATCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI Tsp45I TspRI