Restriction map for
pF1KE3547
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# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AcsI AflII ApoI
AscI AseI AsnI Asp700I AspI
AvrII BbeI BciVI BfrI BlnI
BsaBI BseAI BsiWI BsmI BspEI
Bst1107I BstBI BstZ17I CpoI DraI
EcoRI EcoT22I FseI HindIII HpaI
KasI MamI MfeI MluI MroI
MunI NarI NdeI NheI NruI
NsiI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I RsrII SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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Acc65I 1987 GGTACC G^GTACC
AgeI 3914 ACCGGT A^CCGGT
Alw44I 5707 GTGCAC G^TGCAC
ApaLI 5707 GTGCAC G^TGCAC
Asp718I 1987 GGTACC G^GTACC
BclI 3617 TGATCA T^GATCA
BglII 3335 AGATCT A^GATCT
Bsp1407I 4001 TGTACA T^GTACA
BspDI 3493 ATCGAT AT^CGAT
BspLU11I 5552 ACATGT A^CATGT
BsrGI 4001 TGTACA T^GTACA
BssHII 4599 GCGCGC G^CGCGC
BstEII 4876 GGTGACC G^GT[ACGT]ACC
BstPI 4876 GGTGACC G^GT[ACGT]ACC
ClaI 3493 ATCGAT AT^CGAT
DraIII 2105 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 4876 GGTGACC G^GT[ACGT]ACC
FbaI 3617 TGATCA T^GATCA
KpnI 1987 GGTACC GGTAC^C
MseI 937 TTAA T^TAA
NotI 325 GCGGCCGC GC^GGCCGC
PaeR7I 3007 CTCGAG C^TCGAG
PinAI 3914 ACCGGT A^CCGGT
PvuI 3896 CGATCG CGAT^CG
SspBI 4001 TGTACA T^GTACA
Tru9I 937 TTAA T^TAA
XbaI 4437 TCTAGA T^CTAGA
XhoI 3007 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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Aor51HI 59 AGCGCT AGC^GCT
5775 AGCGCT
AviII 386 TGCGCA TGC^GCA
3254 TGCGCA
BamHI 1740 GGATCC G^GATCC
3635 GGATCC
BbsI 4066 GAAGAC GAAGAC(2/6)
4926 GTCTTC
BlpI 3742 GCTGAGC GC^T[ACGT]AGC
4590 GCTGAGC
Bpu1102I 3742 GCTGAGC GC^T[ACGT]AGC
4590 GCTGAGC
BpuAI 4066 GAAGAC GAAGAC(2/6)
4926 GTCTTC
BspHI 3614 TCATGA T^CATGA
4469 TCATGA
BssSI 3370 CACGAG CACGAG(-5/-1)
126 CTCGTG
BstXI 2040 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5114 CCAGCCCAATGG
CelII 3742 GCTGAGC GC^T[ACGT]AGC
4590 GCTGAGC
DrdI 307 GACGGAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3937 GACCTCATCGTC
Eco47III 59 AGCGCT AGC^GCT
5775 AGCGCT
EcoRV 1396 GATATC GAT^ATC
2384 GATATC
FspI 386 TGCGCA TGC^GCA
3254 TGCGCA
HincII 1367 GTCGAC GT[CT]^[GA]AC
5194 GTCGAC
HindII 1367 GTCGAC GT[CT]^[GA]AC
5194 GTCGAC
NaeI 131 GCCGGC GCC^GGC
593 GCCGGC
NgoMIV 131 GCCGGC G^CCGGC
593 GCCGGC
RcaI 3614 TCATGA T^CATGA
4469 TCATGA
SalI 1367 GTCGAC G^TCGAC
5194 GTCGAC
SapI 2242 GAAGAGC GCTCTTC(1/4)
393 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvCI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI