Restriction map for
pF1KE2527
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AccII AccIII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BglI BglII BlnI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoT22I FseI FspI HaeII HapII
HgaI HpaI HpaII KasI KpnI
KspI MluI MroI MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PvuI RsrII
SacII SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 18 enzymes) #
###################################
AatII 4097 GACGTC GACGT^C
AcyI 4097 GACGTC G[GA]^CG[CT]C
BsaAI 2720 TACGTG [CT]AC^GT[GA]
BsaBI 4222 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4097 GACGTC G[GA]^CG[CT]C
BstAPI 460 GCAGCGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 2976 GCGC GCG^C
DrdI 534 GACTGATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4490 CCATGG C^C[GA][CT]GG
EcoRV 1305 GATATC GAT^ATC
HhaI 2976 GCGC GCG^C
Hin1I 4097 GACGTC G[GA]^CG[CT]C
HinP1I 2976 GCGC G^CGC
MamI 4222 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 4490 CCATGG C^CATGG
PshAI 4687 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SpeI 1315 ACTAGT A^CTAGT
XcmI 4469 CCATGAATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AclI 69 AACGTT AA^CGTT
2706 AACGTT
AlwNI 3701 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
5513 CAGCTGCTG
AspI 3264 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5261 GACCTGGTC
BalI 4353 TGGCCA TGG^CCA
5189 TGGCCA
BanII 2224 GAGCTC G[GA]GC[CT]^C
3051 GAGCTC
BclI 290 TGATCA T^GATCA
3464 TGATCA
BseRI 3056 CTCCTC GAGGAG(10/8)
4157 CTCCTC
BsrBI 4266 CCGCTC CCGCTC(-3/-3)
649 GAGCGG
BssSI 1109 CTCGTG CACGAG(-5/-1)
5405 CTCGTG
EcoNI 4406 CCTTTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5314 CCTACACCAGG
EcoRI 431 GAATTC G^AATTC
2085 GAATTC
FbaI 290 TGATCA T^GATCA
3464 TGATCA
MluNI 4353 TGGCCA TGG^CCA
5189 TGGCCA
MscI 4353 TGGCCA TGG^CCA
5189 TGGCCA
PflFI 3264 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5261 GACCTGGTC
Psp1406I 69 AACGTT AA^CGTT
2706 AACGTT
PvuII 2434 CAGCTG CAG^CTG
5513 CAGCTG
SacI 2224 GAGCTC GAGCT^C
3051 GAGCTC
SalI 734 GTCGAC G^TCGAC
4994 GTCGAC
SapI 646 GAAGAGC GCTCTTC(1/4)
1429 GAAGAGC
StuI 1976 AGGCCT AGG^CCT
4445 AGGCCT
Tth111I 3264 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5261 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BbsI BbvI
BfaI BfrI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraI DraIII EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HincII HindII HindIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MunI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PshBI PstI RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI