Restriction map for
pF1KE2569
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AocI AscI Asp718I AspEI AvrII
BamHI BbvCI BcgI BglII BlnI
BsiEI BsiWI BspDI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoRI FseI HpaI KpnI
MluI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI SalI SbfI
SfiI SfuI SgrAI SnaBI SphI
Sse8387I StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 2462 GTAGAC GT^[AC][GT]AC
AccIII 2679 TCCGGA T^CCGGA
AflII 1617 CTTAAG C^TTAAG
Alw44I 3168 GTGCAC G^TGCAC
Aor51HI 2854 AGCGCT AGC^GCT
ApaI 795 GGGCCC GGGCC^C
ApaLI 3168 GTGCAC G^TGCAC
AspI 2650 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2190 TGCGCA TGC^GCA
BbeI 20 GGCGCC GGCGC^C
BfrI 1617 CTTAAG C^TTAAG
BglI 2886 GCCCGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 2679 TCCGGA T^CCGGA
BsgI 1849 GTGCAG GTGCAG(16/14)
Bsp1407I 440 TGTACA T^GTACA
BspEI 2679 TCCGGA T^CCGGA
BspHI 2294 TCATGA T^CATGA
BspMI 32 ACCTGC ACCTGC(4/8)
BsrFI 744 GCCGGC [GA]^CCGG[CT]
BsrGI 440 TGTACA T^GTACA
BssSI 1858 CTCGTG CACGAG(-5/-1)
BstAPI 2895 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3123 GGTTACC G^GT[ACGT]ACC
BstPI 3123 GGTTACC G^GT[ACGT]ACC
Cfr10I 744 GCCGGC [GA]^CCGG[CT]
CpoI 1472 CGGTCCG CG^G[AT]CCG
DraI 1356 TTTAAA TTT^AAA
DraIII 1366 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2854 AGCGCT AGC^GCT
EcoO65I 3123 GGTTACC G^GT[ACGT]ACC
EcoT22I 990 ATGCAT ATGCA^T
FspI 2190 TGCGCA TGC^GCA
HincII 713 GTCAAC GT[CT]^[GA]AC
HindII 713 GTCAAC GT[CT]^[GA]AC
HindIII 2151 AAGCTT A^AGCTT
KasI 20 GGCGCC G^GCGCC
KspI 79 CCGCGG CCGC^GG
MfeI 1679 CAATTG C^AATTG
MroI 2679 TCCGGA T^CCGGA
MunI 1679 CAATTG C^AATTG
NaeI 744 GCCGGC GCC^GGC
NarI 20 GGCGCC GG^CGCC
NgoMIV 744 GCCGGC G^CCGGC
NsiI 990 ATGCAT ATGCA^T
PflFI 2650 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2346 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 2226 CAGCTG CAG^CTG
RcaI 2294 TCATGA T^CATGA
RsrII 1472 CGGTCCG CG^G[AT]CCG
SacII 79 CCGCGG CCGC^GG
SapI 643 GCTCTTC GCTCTTC(1/4)
ScaI 1758 AGTACT AGT^ACT
SfoI 20 GGCGCC GGC^GCC
SpeI 930 ACTAGT A^CTAGT
SspBI 440 TGTACA T^GTACA
SspI 2474 AATATT AAT^ATT
Tth111I 2650 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2346 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 20 GGCGCC G[GA]^CG[CT]C
469 GACGCC
AseI 1690 ATTAAT AT^TAAT
2070 ATTAAT
AsnI 1690 ATTAAT AT^TAAT
2070 ATTAAT
BbrPI 2587 CACGTG CAC^GTG
3165 CACGTG
BciVI 361 GGATAC GTATCC(6/5)
3160 GGATAC
BfaI 931 CTAG C^TAG
1762 CTAG
BlpI 516 GCTTAGC GC^T[ACGT]AGC
2593 GCTGAGC
Bpu1102I 516 GCTTAGC GC^T[ACGT]AGC
2593 GCTGAGC
BsaAI 2587 CACGTG [CT]AC^GT[GA]
3165 CACGTG
BsaBI 273 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2286 GATGTTGATC
BsaHI 20 GGCGCC G[GA]^CG[CT]C
469 GACGCC
BsaI 2382 GAGACC GGTCTC(1/5)
2536 GAGACC
BsaWI 2679 TCCGGA [AT]^CCGG[AT]
3100 ACCGGA
BspLU11I 1091 ACATGT A^CATGT
2090 ACATGT
BstYI 569 AGATCC [GA]^GATC[CT]
2036 GGATCT
CelII 516 GCTTAGC GC^T[ACGT]AGC
2593 GCTGAGC
Hin1I 20 GGCGCC G[GA]^CG[CT]C
469 GACGCC
MaeI 931 CTAG C^TAG
1762 CTAG
MamI 273 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2286 GATGTTGATC
MflI 569 AGATCC [GA]^GATC[CT]
2036 GGATCT
MspA1I 79 CCGCGG C[AC]G^C[GT]G
2226 CAGCTG
PmaCI 2587 CACGTG CAC^GTG
3165 CACGTG
PmlI 2587 CACGTG CAC^GTG
3165 CACGTG
PshBI 1690 ATTAAT AT^TAAT
2070 ATTAAT
SacI 949 GAGCTC GAGCT^C
3035 GAGCTC
SmaI 86 CCCGGG CCC^GGG
921 CCCGGG
SmlI 1617 CTTAAG C^T[CT][GA]AG
1711 CTCAAG
TaqI 293 TCGA T^CGA
3177 TCGA
TthHB8I 293 TCGA T^CGA
3177 TCGA
XcmI 1242 CCACATGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1440 CCACACCCTTGATGG
XhoII 569 AGATCC [GA]^GATC[CT]
2036 GGATCT
XmaCI 86 CCCGGG C^CCGGG
921 CCCGGG
XmaI 86 CCCGGG C^CCGGG
921 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BclI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoRV EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI