Restriction map for
pF1KB4096
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FauI FbaI FseI FspI Hin1I
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI TfiI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AlwNI 2790 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 2369 CTCGGG C^[CT]CG[GA]G
BalI 860 TGGCCA TGG^CCA
BamHI 1664 GGATCC G^GATCC
BsaWI 420 ACCGGA [AT]^CCGG[AT]
BsmI 2749 GAATGC GAATGC(1/-1)
BsoBI 2369 CTCGGG C^[CT]CG[GA]G
BspDI 1639 ATCGAT AT^CGAT
BstAPI 2744 GCAGTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 1639 ATCGAT AT^CGAT
DraI 1746 TTTAAA TTT^AAA
DrdI 1710 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 860 TGGCCA [CT]^GGCC[GA]
EcoT22I 293 ATGCAT ATGCA^T
HindIII 988 AAGCTT A^AGCTT
HpaI 181 GTTAAC GTT^AAC
MluNI 860 TGGCCA TGG^CCA
MscI 860 TGGCCA TGG^CCA
NcoI 2424 CCATGG C^CATGG
NdeI 2563 CATATG CA^TATG
NsiI 293 ATGCAT ATGCA^T
SapI 829 GAAGAGC GCTCTTC(1/4)
SphI 1535 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 559 GTAGAC GT^[AC][GT]AC
2066 GTAGAC
Asp700I 99 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
702 GAATGAGTTC
BglII 167 AGATCT A^GATCT
1520 AGATCT
BsmBI 1474 CGTCTC CGTCTC(1/5)
2339 GAGACG
Bsp1407I 1182 TGTACA T^GTACA
2591 TGTACA
BsrGI 1182 TGTACA T^GTACA
2591 TGTACA
BstXI 434 CCATCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
785 CCAGGGAACTGG
DraIII 274 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
936 CACCCTGTG
DsaI 505 CCACGG C^C[GA][CT]GG
2424 CCATGG
EcoNI 153 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2700 CCTCAGTGAGG
EcoRI 412 GAATTC G^AATTC
1783 GAATTC
EcoRV 1546 GATATC GAT^ATC
1906 GATATC
HaeII 328 GGCGCT [GA]GCGC^[CT]
2731 GGCGCT
HapII 210 CCGG C^CGG
421 CCGG
HgaI 1532 GACGC GACGC(5/10)
1473 GCGTC
HincII 25 GTCAAC GT[CT]^[GA]AC
181 GTTAAC
HindII 25 GTCAAC GT[CT]^[GA]AC
181 GTTAAC
HpaII 210 CCGG C^CGG
421 CCGG
MspA1I 363 CAGCGG C[AC]G^C[GT]G
522 CCGCTG
MspI 210 CCGG C^CGG
421 CCGG
NspI 1535 GCATGC [GA]CATG^[CT]
1853 ACATGC
PflMI 852 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
935 CCACCCTGTGG
PstI 89 CTGCAG CTGCA^G
1516 CTGCAG
SspBI 1182 TGTACA T^GTACA
2591 TGTACA
StuI 101 AGGCCT AGG^CCT
2675 AGGCCT
Van91I 852 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
935 CCACCCTGTGG
XmnI 99 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
702 GAATGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII