Restriction map for
pF1KE2590
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII AccI AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspI AviII AvrII
BalI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FseI FspI HindIII HpaI
KspI MamI MfeI MluI MluNI
MscI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI PvuI RsrII
SacI SacII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XcmI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 1059 CGCG CG^CG
AccIII 1478 TCCGGA T^CCGGA
AclI 813 AACGTT AA^CGTT
AcyI 1635 GGCGCC G[GA]^CG[CT]C
AflIII 746 ACGTGT A^C[GA][CT]GT
AhdI 2224 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1111 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2224 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1635 GGCGCC GGCGC^C
BbrPI 2218 CACGTG CAC^GTG
BclI 23 TGATCA T^GATCA
BglI 49 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1635 GGCGCC G[GA]^CG[CT]C
BsaI 1406 GAGACC GGTCTC(1/5)
BseAI 1478 TCCGGA T^CCGGA
BspEI 1478 TCCGGA T^CCGGA
BsrDI 2310 CATTGC GCAATG(2/0)
BstUI 1059 CGCG CG^CG
Eam1105I 2224 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 147 GATATC GAT^ATC
FbaI 23 TGATCA T^GATCA
HgaI 2252 GCGTC GACGC(5/10)
Hin1I 1635 GGCGCC G[GA]^CG[CT]C
HincII 1609 GTCAAC GT[CT]^[GA]AC
HindII 1609 GTCAAC GT[CT]^[GA]AC
KasI 1635 GGCGCC G^GCGCC
MroI 1478 TCCGGA T^CCGGA
MvnI 1059 CGCG CG^CG
NarI 1635 GGCGCC GG^CGCC
NspI 822 ACATGC [GA]CATG^[CT]
PmaCI 2218 CACGTG CAC^GTG
PmlI 2218 CACGTG CAC^GTG
Psp1406I 813 AACGTT AA^CGTT
PvuII 38 CAGCTG CAG^CTG
SapI 1689 GCTCTTC GCTCTTC(1/4)
ScaI 1697 AGTACT AGT^ACT
SexAI 2261 ACCTGGT A^CC[AT]GGT
SfoI 1635 GGCGCC GGC^GCC
SmaI 1658 CCCGGG CCC^GGG
TfiI 1457 GAATC G^A[AT]TC
XbaI 1119 TCTAGA T^CTAGA
XmaCI 1658 CCCGGG C^CCGGG
XmaI 1658 CCCGGG C^CCGGG
XmnI 1111 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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Acc65I 1643 GGTACC G^GTACC
2327 GGTACC
AcsI 1197 GAATTT [GA]^AATT[CT]
1278 AAATTC
AlwNI 1600 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2168 CAGGAACTG
ApoI 1197 GAATTT [GA]^AATT[CT]
1278 AAATTC
Asp718I 1643 GGTACC G^GTACC
2327 GGTACC
AspHI 312 GAGCAC G[AT]GC[AT]^C
2215 GAGCAC
BamHI 405 GGATCC G^GATCC
531 GGATCC
BanII 676 GAGCCC G[GA]GC[CT]^C
756 GGGCTC
BbvCI 2247 CCTCAGC CCTCAGC(-5/-2)
1810 GCTGAGG
BcgI 1632 GCAGGCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1928 GCAGAAGGTTCG
BsaAI 1744 TACGTG [CT]AC^GT[GA]
2218 CACGTG
BsaWI 731 ACCGGA [AT]^CCGG[AT]
1478 TCCGGA
BsgI 185 CTGCAC GTGCAG(16/14)
983 GTGCAG
BsiHKAI 312 GAGCAC G[AT]GC[AT]^C
2215 GAGCAC
BspHI 1799 TCATGA T^CATGA
2093 TCATGA
BspMI 1260 ACCTGC ACCTGC(4/8)
483 GCAGGT
KpnI 1643 GGTACC GGTAC^C
2327 GGTACC
MseI 1397 TTAA T^TAA
1909 TTAA
MspA1I 38 CAGCTG C[AC]G^C[GT]G
2197 CCGCTG
PaeR7I 496 CTCGAG C^TCGAG
2212 CTCGAG
PpuMI 359 AGGACCC [GA]G^G[AT]CC[CT]
1339 GGGTCCC
PstI 41 CTGCAG CTGCA^G
1021 CTGCAG
RcaI 1799 TCATGA T^CATGA
2093 TCATGA
SfcI 41 CTGCAG C^T[GA][CT]AG
1021 CTGCAG
Tru9I 1397 TTAA T^TAA
1909 TTAA
Tsp509I 1198 AATT ^AATT
1279 AATT
XhoI 496 CTCGAG C^TCGAG
2212 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII