Restriction map for
pF1KE3350
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
ApaI AseI AsnI Asp718I AviII
AvrII BalI BamHI BcgI BciVI
BfrI BlnI BsiWI BsmBI Bsp1407I
BspDI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EcoO65I EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI MfeI
MluI MluNI MscI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 2505 TCCGGA T^CCGGA
AgeI 2347 ACCGGT A^CCGGT
AhdI 787 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2869 CCTCAGG CC^T[ACGT]AGG
AscI 456 GGCGCGCC GG^CGCGCC
AspEI 787 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2676 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 703 CACGTG CAC^GTG
BbvCI 2827 CCTCAGC CCTCAGC(-5/-2)
BclI 944 TGATCA T^GATCA
BsaI 2790 GGTCTC GGTCTC(1/5)
BseAI 2505 TCCGGA T^CCGGA
BspEI 2505 TCCGGA T^CCGGA
BspHI 1127 TCATGA T^CATGA
BspLU11I 2617 ACATGT A^CATGT
Bsu36I 2869 CCTCAGG CC^T[ACGT]AGG
DraI 1512 TTTAAA TTT^AAA
DrdI 2886 GACAGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 787 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2869 CCTCAGG CC^T[ACGT]AGG
EcoNI 750 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3097 GAATTC G^AATTC
FbaI 944 TGATCA T^GATCA
MroI 2505 TCCGGA T^CCGGA
NaeI 3068 GCCGGC GCC^GGC
NgoMIV 3068 GCCGGC G^CCGGC
PflFI 2676 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2347 ACCGGT A^CCGGT
PmaCI 703 CACGTG CAC^GTG
PmlI 703 CACGTG CAC^GTG
RcaI 1127 TCATGA T^CATGA
SacI 382 GAGCTC GAGCT^C
SgrAI 2346 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1322 CCCGGG CCC^GGG
SphI 740 GCATGC GCATG^C
Tth111I 2676 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1322 CCCGGG C^CCGGG
XmaI 1322 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 351 ACACGT A^C[GA][CT]GT
2617 ACATGT
Alw44I 2175 GTGCAC G^TGCAC
2471 GTGCAC
Aor51HI 618 AGCGCT AGC^GCT
630 AGCGCT
ApaLI 2175 GTGCAC G^TGCAC
2471 GTGCAC
Asp700I 1234 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3093 GAAAGAATTC
BanII 56 GAGCCC G[GA]GC[CT]^C
382 GAGCTC
BbeI 13 GGCGCC GGCGC^C
260 GGCGCC
BbsI 951 GTCTTC GAAGAC(2/6)
2598 GTCTTC
BfaI 1267 CTAG C^TAG
1994 CTAG
BglII 2220 AGATCT A^GATCT
2862 AGATCT
BlpI 68 GCTCAGC GC^T[ACGT]AGC
168 GCTGAGC
Bpu1102I 68 GCTCAGC GC^T[ACGT]AGC
168 GCTGAGC
BpuAI 951 GTCTTC GAAGAC(2/6)
2598 GTCTTC
BsaAI 286 TACGTG [CT]AC^GT[GA]
703 CACGTG
BsaBI 1525 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2686 GATGGAAATC
BsmI 1135 GAATGC GAATGC(1/-1)
2085 GAATGC
BsrFI 2347 ACCGGT [GA]^CCGG[CT]
3068 GCCGGC
CelII 68 GCTCAGC GC^T[ACGT]AGC
168 GCTGAGC
Cfr10I 2347 ACCGGT [GA]^CCGG[CT]
3068 GCCGGC
DraIII 2424 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2818 CACATCGTG
EagI 47 CGGCCG C^GGCCG
305 CGGCCG
EclXI 47 CGGCCG C^GGCCG
305 CGGCCG
Eco47III 618 AGCGCT AGC^GCT
630 AGCGCT
Eco52I 47 CGGCCG C^GGCCG
305 CGGCCG
KasI 13 GGCGCC G^GCGCC
260 GGCGCC
KspI 17 CCGCGG CCGC^GG
609 CCGCGG
MaeI 1267 CTAG C^TAG
1994 CTAG
MamI 1525 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2686 GATGGAAATC
NarI 13 GGCGCC GG^CGCC
260 GGCGCC
NspI 740 GCATGC [GA]CATG^[CT]
2617 ACATGT
SacII 17 CCGCGG CCGC^GG
609 CCGCGG
SapI 1933 GAAGAGC GCTCTTC(1/4)
519 GCTCTTC
SfoI 13 GGCGCC GGC^GCC
260 GGCGCC
XmnI 1234 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3093 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII