Restriction map for
pF1KB5943
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AvrII
BalI BbeI BbrPI BbvCI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaHI BsgI BsiEI BsiWI
BsmBI BspDI BspHI BspLU11I BspMI
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FbaI
FseI Hin1I HindIII HpaI KasI
KpnI KspI MaeII MluI MluNI
MscI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AgeI 345 ACCGGT A^CCGGT
AlwNI 581 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 892 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 237 CTCGGG C^[CT]CG[GA]G
AvaII 653 GGTCC G^G[AT]CC
AviII 1479 TGCGCA TGC^GCA
BamHI 3 GGATCC G^GATCC
BanI 58 GGTGCC G^G[CT][GA]CC
BbvI 580 GCAGC GCAGC(8/12)
BcgI 1317 CGAGCGTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1169 CTAG C^TAG
BglI 61 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 446 AGATCT A^GATCT
BpmI 253 CTGGAG CTGGAG(16/14)
BsaBI 734 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 18 GTCCC GGGAC(10/14)
BsoBI 237 CTCGGG C^[CT]CG[GA]G
Bsp1407I 438 TGTACA T^GTACA
BsrBI 1393 GAGCGG CCGCTC(-3/-3)
BsrFI 345 ACCGGT [GA]^CCGG[CT]
BsrGI 438 TGTACA T^GTACA
BssSI 11 CACGAG CACGAG(-5/-1)
BstXI 1286 CCATCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 345 ACCGGT [GA]^CCGG[CT]
DraI 480 TTTAAA TTT^AAA
DraII 411 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 1507 CCGTGG C^C[GA][CT]GG
EcoO109I 411 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 638 GAATTC G^AATTC
EcoRV 734 GATATC GAT^ATC
EcoT14I 656 CCAAGG C^C[AT][AT]GG
EcoT22I 327 ATGCAT ATGCA^T
Fnu4HI 580 GCAGC GC^[ACGT]GC
FspI 1479 TGCGCA TGC^GCA
HaeII 1376 GGCGCT [GA]GCGC^[CT]
HincII 1143 GTCAAC GT[CT]^[GA]AC
HindII 1143 GTCAAC GT[CT]^[GA]AC
ItaI 580 GCAGC GC^[ACGT]GC
MaeI 1169 CTAG C^TAG
MamI 734 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 431 CAATTG C^AATTG
MunI 431 CAATTG C^AATTG
NsiI 327 ATGCAT ATGCA^T
PflFI 892 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 345 ACCGGT A^CCGGT
SapI 1069 GAAGAGC GCTCTTC(1/4)
SspBI 438 TGTACA T^GTACA
StyI 656 CCAAGG C^C[AT][AT]GG
TseI 580 GCAGC G^C[AT]GC
Tth111I 892 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 969 TCCGGA T^CCGGA
1001 TCCGGA
AfaI 277 GTAC GT^AC
439 GTAC
AspHI 196 GAGCTC G[AT]GC[AT]^C
298 GAGCTC
BcnI 47 CCCGG CC^[GC]GG
1355 CCCGG
BsaI 42 GAGACC GGTCTC(1/5)
1367 GAGACC
BseAI 969 TCCGGA T^CCGGA
1001 TCCGGA
BsiHKAI 196 GAGCTC G[AT]GC[AT]^C
298 GAGCTC
BsmI 325 GAATGC GAATGC(1/-1)
860 GAATGC
BspEI 969 TCCGGA T^CCGGA
1001 TCCGGA
BstYI 3 GGATCC [GA]^GATC[CT]
446 AGATCT
CfoI 1377 GCGC GCG^C
1480 GCGC
FauI 140 CCCGC CCCGC(4/6)
167 CCCGC
HgaI 16 GCGTC GACGC(5/10)
1320 GCGTC
HhaI 1377 GCGC GCG^C
1480 GCGC
HinP1I 1377 GCGC G^CGC
1480 GCGC
MflI 3 GGATCC [GA]^GATC[CT]
446 AGATCT
MroI 969 TCCGGA T^CCGGA
1001 TCCGGA
MslI 615 CATCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1287 CATCCGTGTG
MspA1I 900 CAGCTG C[AC]G^C[GT]G
1257 CAGCTG
NciI 47 CCCGG CC^[GC]GG
1355 CCCGG
PvuII 900 CAGCTG CAG^CTG
1257 CAGCTG
RsaI 277 GTAC GT^AC
439 GTAC
SacI 196 GAGCTC GAGCT^C
298 GAGCTC
SmlI 24 CTTGAG C^T[CT][GA]AG
80 CTCAAG
TaqI 305 TCGA T^CGA
323 TCGA
TfiI 205 GATTC G^A[AT]TC
1404 GATTC
TthHB8I 305 TCGA T^CGA
323 TCGA
XhoII 3 GGATCC [GA]^GATC[CT]
446 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I BanII BbsI BmyI BpuAI
BsaJI BsaWI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII FokI HaeIII HapII
HinfI HpaII HphI Ksp632I MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
Tru9I Tsp45I Tsp509I TspRI XmnI