Restriction map for
pF1KE2554
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstBI BstUI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoT14I FbaI FseI
HgaI HindIII HpaI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 997 GAATTT [GA]^AATT[CT]
AcyI 624 GGCGCC G[GA]^CG[CT]C
AocI 25 CCTGAGG CC^T[ACGT]AGG
ApoI 997 GAATTT [GA]^AATT[CT]
AviII 950 TGCGCA TGC^GCA
BanI 624 GGCGCC G^G[CT][GA]CC
BbeI 624 GGCGCC GGCGC^C
BbsI 733 GTCTTC GAAGAC(2/6)
BglI 874 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 316 CTGGAG CTGGAG(16/14)
BpuAI 733 GTCTTC GAAGAC(2/6)
BsaHI 624 GGCGCC G[GA]^CG[CT]C
BsaI 763 GAGACC GGTCTC(1/5)
BsaJI 1019 CCCTGG C^C[ACGT][ACGT]GG
BsiWI 298 CGTACG C^GTACG
BsrDI 1053 CATTGC GCAATG(2/0)
BsrFI 604 ACCGGC [GA]^CCGG[CT]
BssSI 859 CACGAG CACGAG(-5/-1)
BstAPI 365 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 834 GGTTACC G^GT[ACGT]ACC
BstPI 834 GGTTACC G^GT[ACGT]ACC
Bsu36I 25 CCTGAGG CC^T[ACGT]AGG
Cfr10I 604 ACCGGC [GA]^CCGG[CT]
DraII 190 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eco81I 25 CCTGAGG CC^T[ACGT]AGG
EcoO109I 190 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 834 GGTTACC G^GT[ACGT]ACC
EcoRV 976 GATATC GAT^ATC
EcoT22I 266 ATGCAT ATGCA^T
FspI 950 TGCGCA TGC^GCA
HaeII 624 GGCGCC [GA]GCGC^[CT]
Hin1I 624 GGCGCC G[GA]^CG[CT]C
HincII 892 GTCAAC GT[CT]^[GA]AC
HindII 892 GTCAAC GT[CT]^[GA]AC
HinfI 348 GATTC G^A[ACGT]TC
KasI 624 GGCGCC G^GCGCC
MaeII 731 ACGT A^CGT
NarI 624 GGCGCC GG^CGCC
NsiI 266 ATGCAT ATGCA^T
PstI 172 CTGCAG CTGCA^G
PvuII 339 CAGCTG CAG^CTG
ScaI 377 AGTACT AGT^ACT
SexAI 830 ACCAGGT A^CC[AT]GGT
SfcI 172 CTGCAG C^T[GA][CT]AG
SfoI 624 GGCGCC GGC^GCC
SmlI 844 CTCAAG C^T[CT][GA]AG
SphI 102 GCATGC GCATG^C
StuI 527 AGGCCT AGG^CCT
TaqI 647 TCGA T^CGA
TfiI 348 GATTC G^A[AT]TC
Tsp509I 998 AATT ^AATT
TthHB8I 647 TCGA T^CGA
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# TWO CUT ENZYMES ( 7 enzymes) #
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AlwI 303 GATCC GGATC(4/5)
636 GATCC
Asp700I 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
561 GAAAAACTTC
BsmAI 649 GAGAC GTCTC(1/5)
763 GAGAC
BsmFI 93 GGGAC GGGAC(10/14)
233 GGGAC
EaeI 515 TGGCCG [CT]^GGCC[GA]
1058 CGGCCA
MspA1I 339 CAGCTG C[AC]G^C[GT]G
741 CCGCTG
XmnI 525 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
561 GAAAAACTTC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI Alw44I ApaLI
AspHI AvaII BanII BbvI BcnI
BmyI BseRI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI