Restriction map for
pF1KE5778
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# NO CUT ENZYMES ( 131 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbvCI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiEI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NarI
NcoI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 427 GACGTC GACGT^C
AcyI 427 GACGTC G[GA]^CG[CT]C
Aor51HI 322 AGCGCT AGC^GCT
AspI 646 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 348 GGACC G^G[AT]CC
BamHI 204 GGATCC G^GATCC
BbsI 112 GTCTTC GAAGAC(2/6)
BciVI 1102 GGATAC GTATCC(6/5)
BclI 1407 TGATCA T^GATCA
BcnI 700 CCCGG CC^[GC]GG
BfaI 352 CTAG C^TAG
BpuAI 112 GTCTTC GAAGAC(2/6)
BsaHI 427 GACGTC G[GA]^CG[CT]C
BseRI 1088 GAGGAG GAGGAG(10/8)
BsiWI 449 CGTACG C^GTACG
BsmI 403 GAATGC GAATGC(1/-1)
BsrDI 303 GCAATG GCAATG(2/0)
BsrFI 1681 GCCGGC [GA]^CCGG[CT]
BstEII 1206 GGTGACC G^GT[ACGT]ACC
BstPI 1206 GGTGACC G^GT[ACGT]ACC
Cfr10I 1681 GCCGGC [GA]^CCGG[CT]
DraI 1344 TTTAAA TTT^AAA
DraII 1728 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 1198 CCGTGG C^C[GA][CT]GG
Eco47III 322 AGCGCT AGC^GCT
EcoNI 1504 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1728 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1206 GGTGACC G^GT[ACGT]ACC
FauI 1430 GCGGG CCCGC(4/6)
FbaI 1407 TGATCA T^GATCA
Hin1I 427 GACGTC G[GA]^CG[CT]C
HindIII 1045 AAGCTT A^AGCTT
MaeI 352 CTAG C^TAG
MspA1I 975 CCGCTG C[AC]G^C[GT]G
NaeI 1681 GCCGGC GCC^GGC
NciI 700 CCCGG CC^[GC]GG
NdeI 1494 CATATG CA^TATG
NgoMIV 1681 GCCGGC G^CCGGC
PflFI 646 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 209 CCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 846 CTGCAG CTGCA^G
ScaI 131 AGTACT AGT^ACT
StuI 944 AGGCCT AGG^CCT
Tsp509I 1471 AATT ^AATT
Tth111I 646 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 209 CCACCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 578 GTGCAC G^TGCAC
960 GTGCAC
AlwNI 44 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
952 CAGAAACTG
ApaLI 578 GTGCAC G^TGCAC
960 GTGCAC
BcgI 1489 CGACCCATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1788 CGAAGGCTGTGC
BsaAI 447 CACGTA [CT]AC^GT[GA]
1840 TACGTG
BspMI 82 ACCTGC ACCTGC(4/8)
1203 GCAGGT
BsrBI 1286 CCGCTC CCGCTC(-3/-3)
1807 GAGCGG
BssSI 940 CACGAG CACGAG(-5/-1)
1776 CACGAG
BstYI 204 GGATCC [GA]^GATC[CT]
1060 GGATCT
CfoI 125 GCGC GCG^C
323 GCGC
HaeII 124 AGCGCC [GA]GCGC^[CT]
322 AGCGCT
HapII 701 CCGG C^CGG
1682 CCGG
HgaI 1705 GACGC GACGC(5/10)
1177 GCGTC
HhaI 125 GCGC GCG^C
323 GCGC
HinP1I 125 GCGC G^CGC
323 GCGC
HpaII 701 CCGG C^CGG
1682 CCGG
MflI 204 GGATCC [GA]^GATC[CT]
1060 GGATCT
MspI 701 CCGG C^CGG
1682 CCGG
PleI 63 GACTC GAGTC(4/5)
1309 GAGTC
SmlI 398 CTCAAG C^T[CT][GA]AG
1865 CTCAAG
XhoII 204 GGATCC [GA]^GATC[CT]
1060 GGATCT
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AspHI BbvI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I