Restriction map for
pF1KE2564
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbrPI BciVI BclI
BfrI BlnI BsaAI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstXI BstZ17I Bsu36I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeII MfeI MluI MroI MseI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SfuI
SnaBI SpeI SspBI SspI StuI
SwaI Tru9I Tsp509I Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 169 CGCG CG^CG
Alw44I 1735 GTGCAC G^TGCAC
ApaLI 1735 GTGCAC G^TGCAC
BamHI 812 GGATCC G^GATCC
BcgI 408 CGATGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1469 CTAG C^TAG
BglII 1152 AGATCT A^GATCT
BsaBI 1599 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 776 TCCGGT [AT]^CCGG[AT]
BseRI 1305 CTCCTC GAGGAG(10/8)
BsrBI 1260 CCGCTC CCGCTC(-3/-3)
BsrDI 1693 GCAATG GCAATG(2/0)
BssSI 1375 CACGAG CACGAG(-5/-1)
BstEII 1282 GGTCACC G^GT[ACGT]ACC
BstPI 1282 GGTCACC G^GT[ACGT]ACC
BstUI 169 CGCG CG^CG
DraIII 1488 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1282 GGTCACC G^GT[ACGT]ACC
EcoRV 1423 GATATC GAT^ATC
HgaI 1290 GCGTC GACGC(5/10)
MaeI 1469 CTAG C^TAG
MamI 1599 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 740 CAGCTG C[AC]G^C[GT]G
MvnI 169 CGCG CG^CG
NdeI 1213 CATATG CA^TATG
NspI 579 GCATGC [GA]CATG^[CT]
PaeR7I 623 CTCGAG C^TCGAG
PvuII 740 CAGCTG CAG^CTG
SacI 703 GAGCTC GAGCT^C
SbfI 975 CCTGCAGG CCTGCA^GG
SexAI 985 ACCTGGT A^CC[AT]GGT
SfiI 927 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1285 CACCGGCG C[GA]^CCGG[CT]G
SmaI 377 CCCGGG CCC^GGG
SphI 579 GCATGC GCATG^C
Sse8387I 975 CCTGCAGG CCTGCA^GG
XcmI 438 CCAGCGCCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 623 CTCGAG C^TCGAG
XmaCI 377 CCCGGG C^CCGGG
XmaI 377 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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ApaI 93 GGGCCC GGGCC^C
877 GGGCCC
AvaI 377 CCCGGG C^[CT]CG[GA]G
623 CTCGAG
BalI 1247 TGGCCA TGG^CCA
1800 TGGCCA
BbeI 499 GGCGCC GGCGC^C
1355 GGCGCC
BbsI 1198 GAAGAC GAAGAC(2/6)
1483 GAAGAC
BbvCI 742 GCTGAGG CCTCAGC(-5/-2)
1453 GCTGAGG
BglI 443 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
928 GCCATGGTGGC
BlpI 1253 GCTCAGC GC^T[ACGT]AGC
1262 GCTCAGC
Bpu1102I 1253 GCTCAGC GC^T[ACGT]AGC
1262 GCTCAGC
BpuAI 1198 GAAGAC GAAGAC(2/6)
1483 GAAGAC
BsoBI 377 CCCGGG C^[CT]CG[GA]G
623 CTCGAG
BspMI 575 ACCTGC ACCTGC(4/8)
1093 ACCTGC
BstAPI 59 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
200 GCACCATCTGC
CelII 1253 GCTCAGC GC^T[ACGT]AGC
1262 GCTCAGC
DrdI 460 GACTCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1047 GACTGAGCTGTC
EaeI 1247 TGGCCA [CT]^GGCC[GA]
1800 TGGCCA
EarI 1234 GAAGAG CTCTTC(1/4)
1768 GAAGAG
EcoNI 304 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1094 CCTGCAAGAGG
FauI 1112 CCCGC CCCGC(4/6)
1259 CCCGC
KasI 499 GGCGCC G^GCGCC
1355 GGCGCC
Ksp632I 1234 GAAGAG CTCTTC(1/4)
1768 GAAGAG
MluNI 1247 TGGCCA TGG^CCA
1800 TGGCCA
MscI 1247 TGGCCA TGG^CCA
1800 TGGCCA
NarI 499 GGCGCC GG^CGCC
1355 GGCGCC
NcoI 929 CCATGG C^CATGG
1383 CCATGG
SfoI 499 GGCGCC GGC^GCC
1355 GGCGCC
SmlI 333 CTTGAG C^T[CT][GA]AG
623 CTCGAG
TfiI 1301 GATTC G^A[AT]TC
1727 GAATC
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XhoII