Restriction map for
pF1KB5854
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssHII Bst1107I BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NcoI NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 235 GAATTC [GA]^AATT[CT]
AflIII 1202 ACATGT A^C[GA][CT]GT
AgeI 935 ACCGGT A^CCGGT
Alw44I 904 GTGCAC G^TGCAC
ApaLI 904 GTGCAC G^TGCAC
ApoI 235 GAATTC [GA]^AATT[CT]
BbsI 1068 GAAGAC GAAGAC(2/6)
BcnI 1434 CCCGG CC^[GC]GG
BglI 34 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1138 CTGGAG CTGGAG(16/14)
BpuAI 1068 GAAGAC GAAGAC(2/6)
BsaAI 1150 TACGTG [CT]AC^GT[GA]
BseRI 886 GAGGAG GAGGAG(10/8)
BspLU11I 1202 ACATGT A^CATGT
BsrBI 1083 GAGCGG CCGCTC(-3/-3)
BstBI 346 TTCGAA TT^CGAA
BstEII 1362 GGTCACC G^GT[ACGT]ACC
BstPI 1362 GGTCACC G^GT[ACGT]ACC
BstXI 698 CCAGGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1349 GGATCT [GA]^GATC[CT]
DraIII 907 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 456 CCGCGG C^C[GA][CT]GG
EcoO65I 1362 GGTCACC G^GT[ACGT]ACC
EcoRI 235 GAATTC G^AATTC
KspI 456 CCGCGG CCGC^GG
MflI 1349 GGATCT [GA]^GATC[CT]
MseI 1459 TTAA T^TAA
NciI 1434 CCCGG CC^[GC]GG
NdeI 605 CATATG CA^TATG
NspI 1202 ACATGT [GA]CATG^[CT]
NspV 346 TTCGAA TT^CGAA
PinAI 935 ACCGGT A^CCGGT
PleI 1048 GAGTC GAGTC(4/5)
PstI 384 CTGCAG CTGCA^G
SacII 456 CCGCGG CCGC^GG
SfcI 384 CTGCAG C^T[GA][CT]AG
SfuI 346 TTCGAA TT^CGAA
StuI 485 AGGCCT AGG^CCT
Tru9I 1459 TTAA T^TAA
XcmI 92 CCAACAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1349 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 202 CGCG CG^CG
457 CGCG
AcyI 5 GGCGCC G[GA]^CG[CT]C
1014 GGCGCC
AfaI 462 GTAC GT^AC
1344 GTAC
AlwNI 523 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
745 CAGGCCCTG
ApaI 502 GGGCCC GGGCC^C
1260 GGGCCC
BanII 502 GGGCCC G[GA]GC[CT]^C
1260 GGGCCC
BbeI 5 GGCGCC GGCGC^C
1014 GGCGCC
BsaHI 5 GGCGCC G[GA]^CG[CT]C
1014 GGCGCC
BsaWI 869 ACCGGA [AT]^CCGG[AT]
935 ACCGGT
BsgI 13 CTGCAC GTGCAG(16/14)
327 CTGCAC
BsmI 718 GAATGC GAATGC(1/-1)
491 GCATTC
BstUI 202 CGCG CG^CG
457 CGCG
EcoT14I 1358 CCAAGG C^C[AT][AT]GG
1415 CCAAGG
HaeII 5 GGCGCC [GA]GCGC^[CT]
1014 GGCGCC
HgaI 666 GACGC GACGC(5/10)
1062 GACGC
Hin1I 5 GGCGCC G[GA]^CG[CT]C
1014 GGCGCC
KasI 5 GGCGCC G^GCGCC
1014 GGCGCC
MvnI 202 CGCG CG^CG
457 CGCG
NaeI 1112 GCCGGC GCC^GGC
1213 GCCGGC
NarI 5 GGCGCC GG^CGCC
1014 GGCGCC
NgoMIV 1112 GCCGGC G^CCGGC
1213 GCCGGC
PvuII 1164 CAGCTG CAG^CTG
1311 CAGCTG
RsaI 462 GTAC GT^AC
1344 GTAC
SfoI 5 GGCGCC GGC^GCC
1014 GGCGCC
StyI 1358 CCAAGG C^C[AT][AT]GG
1415 CCAAGG
TfiI 344 GATTC G^A[AT]TC
848 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AluI AlwI Asp700I AspHI
AvaI AvaII BanI BbvI BmyI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI