Restriction map for
pF1KE2456
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspI AviII
BalI BanI BbeI BcgI BciVI
BcnI BfrI BglI BlpI Bpu1102I
BsaHI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BspMI BsrGI BssHII BssSI
BstAPI BstBI BstUI CelII ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HaeII Hin1I HindIII KasI KpnI
KspI MluI MluNI MscI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PflFI PmeI Psp1406I PstI PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI Tth111I XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 160 TCCGGA T^CCGGA
AgeI 4928 ACCGGT A^CCGGT
AhdI 4924 GACCACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4924 GACCACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5023 CCTAGG C^CTAGG
BamHI 3834 GGATCC G^GATCC
BbrPI 4558 CACGTG CAC^GTG
BlnI 5023 CCTAGG C^CTAGG
BsaAI 4558 CACGTG [CT]AC^GT[GA]
BsaI 4364 GAGACC GGTCTC(1/5)
BseAI 160 TCCGGA T^CCGGA
BsmBI 1912 CGTCTC CGTCTC(1/5)
BspEI 160 TCCGGA T^CCGGA
BsrBI 1612 CCGCTC CCGCTC(-3/-3)
BsrFI 4928 ACCGGT [GA]^CCGG[CT]
BstXI 2694 CCATCGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 3264 GCGC GCG^C
Cfr10I 4928 ACCGGT [GA]^CCGG[CT]
DsaI 3343 CCACGG C^C[GA][CT]GG
EaeI 281 TGGCCG [CT]^GGCC[GA]
Eam1105I 4924 GACCACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1305 ATGCAT ATGCA^T
FauI 3976 GCGGG CCCGC(4/6)
HgaI 1728 GACGC GACGC(5/10)
HhaI 3264 GCGC GCG^C
HinP1I 3264 GCGC G^CGC
HincII 1767 GTTAAC GT[CT]^[GA]AC
HindII 1767 GTTAAC GT[CT]^[GA]AC
HpaI 1767 GTTAAC GTT^AAC
MfeI 3449 CAATTG C^AATTG
MroI 160 TCCGGA T^CCGGA
MunI 3449 CAATTG C^AATTG
NsiI 1305 ATGCAT ATGCA^T
PacI 955 TTAATTAA TTAAT^TAA
PaeR7I 3455 CTCGAG C^TCGAG
PflMI 2961 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 4928 ACCGGT A^CCGGT
PmaCI 4558 CACGTG CAC^GTG
PmlI 4558 CACGTG CAC^GTG
PpuMI 3018 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 3841 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 4219 GAAGAGC GCTCTTC(1/4)
ScaI 2838 AGTACT AGT^ACT
SexAI 3015 ACCAGGT A^CC[AT]GGT
SpeI 3636 ACTAGT A^CTAGT
Van91I 2961 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2538 TCTAGA T^CTAGA
XhoI 3455 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 796 CCTGAGG CC^T[ACGT]AGG
2234 CCTCAGG
AspHI 1277 GAGCTC G[AT]GC[AT]^C
1954 GAGCTC
BbvCI 2208 CCTCAGC CCTCAGC(-5/-2)
2554 GCTGAGG
BsgI 1260 GTGCAG GTGCAG(16/14)
3914 GTGCAG
BsiHKAI 1277 GAGCTC G[AT]GC[AT]^C
1954 GAGCTC
BsmFI 4622 GGGAC GGGAC(10/14)
975 GTCCC
Bst1107I 3711 GTATAC GTA^TAC
4392 GTATAC
BstEII 901 GGTGACC G^GT[ACGT]ACC
5027 GGTCACC
BstPI 901 GGTGACC G^GT[ACGT]ACC
5027 GGTCACC
BstZ17I 3711 GTATAC GTA^TAC
4392 GTATAC
Bsu36I 796 CCTGAGG CC^T[ACGT]AGG
2234 CCTCAGG
DraII 1241 AGGGCCT [GA]G^G[ACGT]CC[CT]
3018 AGGTCCT
Eco81I 796 CCTGAGG CC^T[ACGT]AGG
2234 CCTCAGG
EcoO109I 1241 AGGGCCT [GA]G^G[ACGT]CC[CT]
3018 AGGTCCT
EcoO65I 901 GGTGACC G^GT[ACGT]ACC
5027 GGTCACC
EcoRI 1941 GAATTC G^AATTC
2314 GAATTC
MspA1I 1208 CAGCTG C[AC]G^C[GT]G
1263 CAGCTG
NspI 1303 GCATGC [GA]CATG^[CT]
4494 GCATGC
PvuII 1208 CAGCTG CAG^CTG
1263 CAGCTG
SacI 1277 GAGCTC GAGCT^C
1954 GAGCTC
SfcI 1936 CTACAG C^T[GA][CT]AG
3029 CTGTAG
SphI 1303 GCATGC GCATG^C
4494 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AvaI AvaII BanII BbsI
BbvI BclI BfaI BglII BmyI
BpmI BpuAI BsaBI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmI
BsoBI Bsp1286I BspHI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoNI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PshBI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI