Restriction map for
pF1KE2604
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfrI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MflI MluI MroI
MseI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 286 AAATTC [GA]^AATT[CT]
AfaI 109 GTAC GT^AC
AlwI 430 GATCC GGATC(4/5)
AlwNI 274 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 286 AAATTC [GA]^AATT[CT]
BalI 192 TGGCCA TGG^CCA
BciVI 91 GGATAC GTATCC(6/5)
BfaI 503 CTAG C^TAG
BglI 46 GCCCGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 313 GAGACC GGTCTC(1/5)
BseRI 498 CTCCTC GAGGAG(10/8)
BsiEI 49 CGACCG CG[GA][CT]^CG
BsrFI 51 ACCGGC [GA]^CCGG[CT]
BssSI 413 CTCGTG CACGAG(-5/-1)
BstXI 189 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 438 GCCCGC GC[ACGT]^[ACGT]GC
Cfr10I 51 ACCGGC [GA]^CCGG[CT]
Cfr13I 377 GGCCC G^G[ACGT]CC
DdeI 394 CTGAG C^T[ACGT]AG
EaeI 192 TGGCCA [CT]^GGCC[GA]
EarI 208 CTCTTC CTCTTC(1/4)
EcoNI 389 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 439 CCCGC CCCGC(4/6)
HapII 52 CCGG C^CGG
HpaII 52 CCGG C^CGG
HphI 330 GGTGA GGTGA(8/7)
Ksp632I 208 CTCTTC CTCTTC(1/4)
MaeI 503 CTAG C^TAG
MboII 209 TCTTC GAAGA(8/7)
MfeI 257 CAATTG C^AATTG
MluNI 192 TGGCCA TGG^CCA
MscI 192 TGGCCA TGG^CCA
MspA1I 440 CCGCTG C[AC]G^C[GT]G
MspI 52 CCGG C^CGG
MunI 257 CAATTG C^AATTG
PshAI 315 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 422 CTGCAG CTGCA^G
RsaI 109 GTAC GT^AC
Sau96I 377 GGCCC G^G[ACGT]CC
SfaNI 152 GCATC GCATC(5/9)
SfcI 422 CTGCAG C^T[GA][CT]AG
Tsp45I 506 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 26 enzymes) #
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BpmI 446 CTCCAG CTGGAG(16/14)
310 CTGGAG
BsmAI 313 GAGAC GTCTC(1/5)
482 GAGAC
BsmFI 519 GGGAC GGGAC(10/14)
322 GTCCC
BssKI 156 CCTGG ^CC[ACGT]GG
528 CCAGG
BstF5I 153 CATCC GGATG(2/0)
168 CATCC
BstNI 156 CCTGG CC^[AT]GG
528 CCAGG
DpnII 345 GATC ^GATC
430 GATC
DsaI 189 CCATGG C^C[GA][CT]GG
195 CCATGG
EcoRII 156 CCTGG ^CC[AT]GG
528 CCAGG
EcoT14I 189 CCATGG C^C[AT][AT]GG
195 CCATGG
FokI 153 CATCC GGATG(9/13)
168 CATCC
HaeIII 193 GGCC GG^CC
377 GGCC
HinfI 175 GAGTC G^A[ACGT]TC
521 GACTC
MaeIII 8 GTAAC ^GT[ACGT]AC
506 GTGAC
MboI 345 GATC ^GATC
430 GATC
MslI 190 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
409 CACTCTCGTG
MvaI 156 CCTGG CC^[AT]GG
528 CCAGG
NcoI 189 CCATGG C^CATGG
195 CCATGG
NdeII 345 GATC ^GATC
430 GATC
PleI 521 GACTC GAGTC(4/5)
175 GAGTC
Sau3AI 345 GATC ^GATC
430 GATC
ScrFI 156 CCTGG CC^[ACGT]GG
528 CCAGG
StyI 189 CCATGG C^C[AT][AT]GG
195 CCATGG
TaqI 405 TCGA T^CGA
428 TCGA
Tsp509I 258 AATT ^AATT
287 AATT
TthHB8I 405 TCGA T^CGA
428 TCGA
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AciI AluI BbvI BsaJI BsiYI
BslI BsrI Fnu4HI ItaI MnlI
MwoI NlaIII NlaIV TseI