Restriction map for
pF1KE2671
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BsaAI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NarI NdeI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Alw44I 826 GTGCAC G^TGCAC
AlwNI 452 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 342 CCTCAGG CC^T[ACGT]AGG
ApaLI 826 GTGCAC G^TGCAC
AspHI 826 GTGCAC G[AT]GC[AT]^C
BanII 477 GGGCTC G[GA]GC[CT]^C
BbsI 82 GAAGAC GAAGAC(2/6)
BfaI 301 CTAG C^TAG
BlpI 732 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 732 GCTGAGC GC^T[ACGT]AGC
BpuAI 82 GAAGAC GAAGAC(2/6)
BsaBI 667 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 601 GAGACC GGTCTC(1/5)
BsiHKAI 826 GTGCAC G[AT]GC[AT]^C
BsmBI 496 CGTCTC CGTCTC(1/5)
BstAPI 378 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 794 CCATCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 282 GGATCT [GA]^GATC[CT]
Bsu36I 342 CCTCAGG CC^T[ACGT]AGG
CelII 732 GCTGAGC GC^T[ACGT]AGC
DraII 941 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 499 CTCTTC CTCTTC(1/4)
Eco81I 342 CCTCAGG CC^T[ACGT]AGG
EcoO109I 941 AGGCCCT [GA]G^G[ACGT]CC[CT]
Ksp632I 499 CTCTTC CTCTTC(1/4)
MaeI 301 CTAG C^TAG
MamI 667 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 282 GGATCT [GA]^GATC[CT]
NaeI 835 GCCGGC GCC^GGC
NcoI 908 CCATGG C^CATGG
NgoMIV 835 GCCGGC G^CCGGC
NheI 300 GCTAGC G^CTAGC
PflMI 451 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 719 CAGCTG CAG^CTG
StuI 161 AGGCCT AGG^CCT
Van91I 451 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 282 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 237 GACGCC G[GA]^CG[CT]C
853 GACGCC
AfaI 90 GTAC GT^AC
420 GTAC
AlwI 192 GGATC GGATC(4/5)
282 GGATC
AvaI 77 CCCGGG C^[CT]CG[GA]G
188 CCCGGG
AvaII 45 GGTCC G^G[AT]CC
266 GGACC
BanI 347 GGTGCC G^G[CT][GA]CC
377 GGCACC
BglI 31 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
448 GCCCCAGTGGC
BpmI 139 CTCCAG CTGGAG(16/14)
964 CTGGAG
BsaHI 237 GACGCC G[GA]^CG[CT]C
853 GACGCC
BsmFI 265 GGGAC GGGAC(10/14)
12 GTCCC
BsoBI 77 CCCGGG C^[CT]CG[GA]G
188 CCCGGG
BsrDI 831 CATTGC GCAATG(2/0)
580 GCAATG
BsrFI 380 ACCGGC [GA]^CCGG[CT]
835 GCCGGC
Cfr10I 380 ACCGGC [GA]^CCGG[CT]
835 GCCGGC
EcoT14I 269 CCAAGG C^C[AT][AT]GG
908 CCATGG
Hin1I 237 GACGCC G[GA]^CG[CT]C
853 GACGCC
MaeII 473 ACGT A^CGT
991 ACGT
MaeIII 470 GTGAC ^GT[ACGT]AC
818 GTGAC
MseI 764 TTAA T^TAA
953 TTAA
MslI 811 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
880 CACTACTATG
RsaI 90 GTAC GT^AC
420 GTAC
SfaNI 406 GATGC GCATC(5/9)
960 GATGC
SmaI 77 CCCGGG CCC^GGG
188 CCCGGG
SmlI 214 CTTGAG C^T[CT][GA]AG
901 CTCAAG
StyI 269 CCAAGG C^C[AT][AT]GG
908 CCATGG
TaqI 95 TCGA T^CGA
524 TCGA
Tru9I 764 TTAA T^TAA
953 TTAA
Tsp45I 470 GTGAC ^GT[GC]AC
818 GTGAC
TthHB8I 95 TCGA T^CGA
524 TCGA
XcmI 32 CCATGTTGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
779 CCACGGGCCTGTTGG
XmaCI 77 CCCGGG C^CCGGG
188 CCCGGG
XmaI 77 CCCGGG C^CCGGG
188 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI BalI BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TfiI
TseI TspRI