Restriction map for
pF1KE2763
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 82 GGCGTC G[GA]^CG[CT]C
AfaI 225 GTAC GT^AC
AluI 104 AGCT AG^CT
AocI 115 CCTGAGG CC^T[ACGT]AGG
BanI 24 GGCACC G^G[CT][GA]CC
BanII 213 GGGCTC G[GA]GC[CT]^C
BbvI 102 GCAGC GCAGC(8/12)
BglI 149 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 326 CTCCAG CTGGAG(16/14)
BsaHI 82 GGCGTC G[GA]^CG[CT]C
BsgI 190 CTGCAC GTGCAG(16/14)
Bsp1407I 224 TGTACA T^GTACA
BspMI 188 ACCTGC ACCTGC(4/8)
BsrGI 224 TGTACA T^GTACA
BstF5I 108 CATCC GGATG(2/0)
Bsu36I 115 CCTGAGG CC^T[ACGT]AGG
DdeI 116 CTGAG C^T[ACGT]AG
DpnII 78 GATC ^GATC
DraII 146 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eco81I 115 CCTGAGG CC^T[ACGT]AGG
EcoNI 111 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 146 GGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 108 CATCC GGATG(9/13)
Hin1I 82 GGCGTC G[GA]^CG[CT]C
HphI 75 GGTGA GGTGA(8/7)
MboI 78 GATC ^GATC
NdeII 78 GATC ^GATC
NspI 98 GCATGC [GA]CATG^[CT]
PstI 19 CTGCAG CTGCA^G
RsaI 225 GTAC GT^AC
Sau3AI 78 GATC ^GATC
SfcI 19 CTGCAG C^T[GA][CT]AG
SphI 98 GCATGC GCATG^C
SspBI 224 TGTACA T^GTACA
TaqI 32 TCGA T^CGA
TseI 102 GCAGC G^C[AT]GC
Tsp509I 296 AATT ^AATT
TspRI 132 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 32 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BmyI 129 GGGCAC G[AGT]GC[ACT]^C
213 GGGCTC
BsaJI 71 CCCTGG C^C[ACGT][ACGT]GG
153 CCTGGG
Bsp1286I 129 GGGCAC G[AGT]GC[ACT]^C
213 GGGCTC
Cfr13I 147 GGGCC G^G[ACGT]CC
237 GGCCC
FauI 41 CCCGC CCCGC(4/6)
142 GCGGG
Fnu4HI 102 GCAGC GC^[ACGT]GC
204 GCCGC
HaeIII 148 GGCC GG^CC
237 GGCC
HgaI 348 GACGC GACGC(5/10)
83 GCGTC
ItaI 102 GCAGC GC^[ACGT]GC
204 GCCGC
MaeII 161 ACGT A^CGT
245 ACGT
MwoI 105 GCTCATCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
149 GCCTCCTGGGC
NlaIII 99 CATG CATG^
174 CATG
Sau96I 147 GGGCC G^G[ACGT]CC
237 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AciI BbsI BpuAI BsiYI BslI
BssKI BstNI Cac8I EcoRII MboII
MnlI MvaI NlaIV ScrFI