Restriction map for
pF1KE2638
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII Cfr10I Cfr13I ClaI
CpoI DpnII DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MboI MfeI MflI MluI MroI
MslI MunI MvnI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI Sau3AI Sau96I SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 492 CCGC CCGC(-3/-1)
AcsI 634 AAATTC [GA]^AATT[CT]
AfaI 428 GTAC GT^AC
AluI 330 AGCT AG^CT
Aor51HI 66 AGCGCT AGC^GCT
ApoI 634 AAATTC [GA]^AATT[CT]
AvaI 416 CCCGGG C^[CT]CG[GA]G
BalI 587 TGGCCA TGG^CCA
BbvCI 81 CCTCAGC CCTCAGC(-5/-2)
BfaI 425 CTAG C^TAG
BsaAI 736 CACGTA [CT]AC^GT[GA]
BsmAI 477 GTCTC GTCTC(1/5)
BsoBI 416 CCCGGG C^[CT]CG[GA]G
BstF5I 710 CATCC GGATG(2/0)
BstNI 441 CCTGG CC^[AT]GG
CfoI 67 GCGC GCG^C
EaeI 587 TGGCCA [CT]^GGCC[GA]
EarI 361 GAAGAG CTCTTC(1/4)
Eco47III 66 AGCGCT AGC^GCT
EcoRII 441 CCTGG ^CC[AT]GG
EcoT14I 399 CCTTGG C^C[AT][AT]GG
FokI 710 CATCC GGATG(9/13)
HaeII 66 AGCGCT [GA]GCGC^[CT]
HapII 417 CCGG C^CGG
HgaI 310 GCGTC GACGC(5/10)
HhaI 67 GCGC GCG^C
HinP1I 67 GCGC G^CGC
HpaII 417 CCGG C^CGG
Ksp632I 361 GAAGAG CTCTTC(1/4)
MaeI 425 CTAG C^TAG
MaeIII 346 GTCAC ^GT[ACGT]AC
MluNI 587 TGGCCA TGG^CCA
MscI 587 TGGCCA TGG^CCA
MspA1I 329 CAGCTG C[AC]G^C[GT]G
MspI 417 CCGG C^CGG
MvaI 441 CCTGG CC^[AT]GG
NlaIII 270 CATG CATG^
PvuII 329 CAGCTG CAG^CTG
RsaI 428 GTAC GT^AC
SmaI 416 CCCGGG CCC^GGG
SspI 547 AATATT AAT^ATT
StuI 396 AGGCCT AGG^CCT
StyI 399 CCTTGG C^C[AT][AT]GG
Tsp45I 346 GTCAC ^GT[GC]AC
XcmI 431 CCAGTGTTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 416 CCCGGG C^CCGGG
XmaI 416 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
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Asp700I 483 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
544 GAAAATATTC
AspHI 247 GTGCTC G[AT]GC[AT]^C
409 GTGCTC
BcnI 416 CCCGG CC^[GC]GG
417 CCGGG
BmyI 247 GTGCTC G[AGT]GC[ACT]^C
409 GTGCTC
BpmI 149 CTCCAG CTGGAG(16/14)
91 CTGGAG
BsaJI 399 CCTTGG C^C[ACGT][ACGT]GG
416 CCCGGG
BsiHKAI 247 GTGCTC G[AT]GC[AT]^C
409 GTGCTC
BsiYI 579 CCTCCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
735 CCACGTATGGG
BslI 579 CCTCCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
735 CCACGTATGGG
BsmFI 453 GGGAC GGGAC(10/14)
41 GTCCC
Bsp1286I 247 GTGCTC G[AGT]GC[ACT]^C
409 GTGCTC
DdeI 82 CTCAG C^T[ACGT]AG
161 CTGAG
DraI 15 TTTAAA TTT^AAA
301 TTTAAA
HphI 215 TCACC GGTGA(8/7)
232 TCACC
MaeII 407 ACGT A^CGT
737 ACGT
MboII 361 GAAGA GAAGA(8/7)
564 TCTTC
MwoI 67 GCGCTAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
310 GCGTCCTTTGC
NciI 416 CCCGG CC^[GC]GG
417 CCGGG
PleI 508 GAGTC GAGTC(4/5)
575 GAGTC
TfiI 284 GAATC G^A[AT]TC
488 GATTC
XmnI 483 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
544 GAAAATATTC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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BbvI BsrI BssKI Fnu4HI HaeIII
HinfI ItaI MnlI MseI ScrFI
SmlI Tru9I TseI Tsp509I TspRI