Restriction map for
pF1KE6677
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MamI MboII MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 167 GGTACC G^GTACC
AcyI 406 GGCGTC G[GA]^CG[CT]C
AflIII 89 ACATGT A^C[GA][CT]GT
AlwI 635 GGATC GGATC(4/5)
Asp718I 167 GGTACC G^GTACC
AvaII 690 GGACC G^G[AT]CC
BanI 167 GGTACC G^G[CT][GA]CC
BfaI 805 CTAG C^TAG
BpmI 475 CTGGAG CTGGAG(16/14)
BsaAI 211 TACGTG [CT]AC^GT[GA]
BsaHI 406 GGCGTC G[GA]^CG[CT]C
BsaI 565 GAGACC GGTCTC(1/5)
BsmI 399 GAATGC GAATGC(1/-1)
Bsp1407I 86 TGTACA T^GTACA
BspHI 743 TCATGA T^CATGA
BspLU11I 89 ACATGT A^CATGT
BspMI 378 GCAGGT ACCTGC(4/8)
BsrGI 86 TGTACA T^GTACA
BsrI 481 ACTGG ACTGG(1/-1)
BstAPI 831 GCAGTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 531 GGATG GGATG(2/0)
BstYI 635 GGATCT [GA]^GATC[CT]
DdeI 277 CTCAG C^T[ACGT]AG
DsaI 152 CCACGG C^C[GA][CT]GG
EcoRI 703 GAATTC G^AATTC
EcoT14I 560 CCTTGG C^C[AT][AT]GG
FokI 531 GGATG GGATG(9/13)
HgaI 407 GCGTC GACGC(5/10)
Hin1I 406 GGCGTC G[GA]^CG[CT]C
HinfI 553 GAATC G^A[ACGT]TC
KpnI 167 GGTACC GGTAC^C
MaeI 805 CTAG C^TAG
MaeII 212 ACGT A^CGT
MaeIII 113 GTGAC ^GT[ACGT]AC
MflI 635 GGATCT [GA]^GATC[CT]
MseI 883 TTAA T^TAA
NspI 89 ACATGT [GA]CATG^[CT]
RcaI 743 TCATGA T^CATGA
SacI 673 GAGCTC GAGCT^C
SfaNI 304 GATGC GCATC(5/9)
SmaI 38 CCCGGG CCC^GGG
SmlI 244 CTCAAG C^T[CT][GA]AG
SspBI 86 TGTACA T^GTACA
StuI 850 AGGCCT AGG^CCT
StyI 560 CCTTGG C^C[AT][AT]GG
TfiI 553 GAATC G^A[AT]TC
Tru9I 883 TTAA T^TAA
Tsp45I 113 GTGAC ^GT[GC]AC
XhoII 635 GGATCT [GA]^GATC[CT]
XmaCI 38 CCCGGG C^CCGGG
XmaI 38 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 703 GAATTC [GA]^AATT[CT]
843 GAATTT
ApoI 703 GAATTC [GA]^AATT[CT]
843 GAATTT
AspHI 58 GTGCTC G[AT]GC[AT]^C
673 GAGCTC
AvaI 38 CCCGGG C^[CT]CG[GA]G
499 CCCGAG
BalI 416 TGGCCA TGG^CCA
773 TGGCCA
BanII 330 GAGCCC G[GA]GC[CT]^C
673 GAGCTC
BsgI 99 CTGCAC GTGCAG(16/14)
370 CTGCAC
BsiHKAI 58 GTGCTC G[AT]GC[AT]^C
673 GAGCTC
BsmAI 478 GAGAC GTCTC(1/5)
565 GAGAC
BsoBI 38 CCCGGG C^[CT]CG[GA]G
499 CCCGAG
DpnII 234 GATC ^GATC
636 GATC
EaeI 416 TGGCCA [CT]^GGCC[GA]
773 TGGCCA
FauI 491 CCCGC CCCGC(4/6)
528 GCGGG
MboI 234 GATC ^GATC
636 GATC
MluNI 416 TGGCCA TGG^CCA
773 TGGCCA
MscI 416 TGGCCA TGG^CCA
773 TGGCCA
MslI 694 CACAAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
744 CATGAAAGTG
MspA1I 124 CAGCTG C[AC]G^C[GT]G
472 CAGCTG
NdeII 234 GATC ^GATC
636 GATC
PstI 256 CTGCAG CTGCA^G
581 CTGCAG
PvuII 124 CAGCTG CAG^CTG
472 CAGCTG
Sau3AI 234 GATC ^GATC
636 GATC
TaqI 443 TCGA T^CGA
726 TCGA
Tsp509I 704 AATT ^AATT
844 AATT
TspRI 131 CACTG CA[GC]TG[ACGT][ACGT]^
434 CAGTG
TthHB8I 443 TCGA T^CGA
726 TCGA
XcmI 471 CCAGCTGGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
740 CCATCATGAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI Cac8I
Cfr13I EcoRII Fnu4HI HaeIII HapII
HpaII HphI ItaI MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
RsaI Sau96I ScrFI SfcI TseI