Restriction map for
pF1KE6561
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmFI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 308 AAATTC [GA]^AATT[CT]
AocI 137 CCTCAGG CC^T[ACGT]AGG
ApoI 308 AAATTC [GA]^AATT[CT]
BanI 359 GGCACC G^G[CT][GA]CC
BcnI 363 CCCGG CC^[GC]GG
BglI 415 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 4 CTCCTC GAGGAG(10/8)
BsiEI 68 CGATCG CG[GA][CT]^CG
BsmBI 60 CGTCTC CGTCTC(1/5)
BsmI 227 GCATTC GAATGC(1/-1)
BspMI 281 GCAGGT ACCTGC(4/8)
BsrDI 92 GCAATG GCAATG(2/0)
BsrI 295 ACTGG ACTGG(1/-1)
BstF5I 408 CATCC GGATG(2/0)
BstXI 172 CCAAACGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 137 CCTCAGG CC^T[ACGT]AGG
Cfr13I 435 GGCCC G^G[ACGT]CC
EaeI 341 CGGCCA [CT]^GGCC[GA]
Eco81I 137 CCTCAGG CC^T[ACGT]AGG
EcoNI 267 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 272 CCAAGG C^C[AT][AT]GG
FokI 408 CATCC GGATG(9/13)
HapII 364 CCGG C^CGG
HinfI 299 GAATC G^A[ACGT]TC
HpaII 364 CCGG C^CGG
MaeII 35 ACGT A^CGT
MslI 453 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 444 CAGCGG C[AC]G^C[GT]G
MspI 364 CCGG C^CGG
NciI 363 CCCGG CC^[GC]GG
NlaIII 204 CATG CATG^
NlaIV 359 GGCACC GG[ACGT]^[ACGT]CC
PstI 184 CTGCAG CTGCA^G
PvuI 68 CGATCG CGAT^CG
Sau96I 435 GGCCC G^G[ACGT]CC
SfaNI 372 GCATC GCATC(5/9)
SfiI 414 GGCCTGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 272 CCAAGG C^C[AT][AT]GG
TaqI 214 TCGA T^CGA
TfiI 299 GAATC G^A[AT]TC
Tsp509I 309 AATT ^AATT
TthHB8I 214 TCGA T^CGA
XcmI 306 CCAAATTCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 10 enzymes) #
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AccII 39 CGCG CG^CG
475 CGCG
AlwI 207 GATCC GGATC(4/5)
83 GGATC
BsmAI 61 GTCTC GTCTC(1/5)
234 GTCTC
BstUI 39 CGCG CG^CG
475 CGCG
EarI 132 CTCTTC CTCTTC(1/4)
259 CTCTTC
Ksp632I 132 CTCTTC CTCTTC(1/4)
259 CTCTTC
MvnI 39 CGCG CG^CG
475 CGCG
MwoI 45 GCTGGTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
415 GCCTGGGTGGC
SfcI 123 CTGTAG C^T[GA][CT]AG
184 CTGCAG
TspRI 154 CACTG CA[GC]TG[ACGT][ACGT]^
294 CACTG
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BssKI BstNI DdeI DpnII EcoRII
Fnu4HI HaeIII HphI ItaI MboI
MboII MnlI MvaI NdeII Sau3AI
ScrFI TseI