Restriction map for
pF1KE2635
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
Tth111I Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AflII 691 CTTAAG C^TTAAG
Asp700I 606 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 820 CTCGAG C^[CT]CG[GA]G
AvaII 915 GGTCC G^G[AT]CC
BalI 947 TGGCCA TGG^CCA
BamHI 1092 GGATCC G^GATCC
BanI 600 GGCACC G^G[CT][GA]CC
BbsI 813 GAAGAC GAAGAC(2/6)
BfrI 691 CTTAAG C^TTAAG
BpmI 164 CTGGAG CTGGAG(16/14)
BpuAI 813 GAAGAC GAAGAC(2/6)
BsaBI 1114 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 323 GGGAC GGGAC(10/14)
BsoBI 820 CTCGAG C^[CT]CG[GA]G
BspHI 524 TCATGA T^CATGA
BspMI 411 ACCTGC ACCTGC(4/8)
BsrBI 823 GAGCGG CCGCTC(-3/-3)
BsrDI 1084 CATTGC GCAATG(2/0)
BstAPI 658 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 145 GGTGACC G^GT[ACGT]ACC
BstPI 145 GGTGACC G^GT[ACGT]ACC
BstYI 1092 GGATCC [GA]^GATC[CT]
Cac8I 835 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 487 GCGC GCG^C
DpnII 1093 GATC ^GATC
DraI 316 TTTAAA TTT^AAA
DsaI 944 CCATGG C^C[GA][CT]GG
EaeI 947 TGGCCA [CT]^GGCC[GA]
EarI 391 CTCTTC CTCTTC(1/4)
EcoO65I 145 GGTGACC G^GT[ACGT]ACC
EcoRI 59 GAATTC G^AATTC
FauI 825 GCGGG CCCGC(4/6)
HaeII 486 GGCGCT [GA]GCGC^[CT]
HapII 898 CCGG C^CGG
HhaI 487 GCGC GCG^C
HinP1I 487 GCGC G^CGC
HpaII 898 CCGG C^CGG
Ksp632I 391 CTCTTC CTCTTC(1/4)
MaeIII 146 GTGAC ^GT[ACGT]AC
MamI 1114 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1093 GATC ^GATC
MflI 1092 GGATCC [GA]^GATC[CT]
MluNI 947 TGGCCA TGG^CCA
MscI 947 TGGCCA TGG^CCA
MspI 898 CCGG C^CGG
NcoI 944 CCATGG C^CATGG
NdeII 1093 GATC ^GATC
PaeR7I 820 CTCGAG C^TCGAG
PshAI 881 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 347 CTGCAG CTGCA^G
RcaI 524 TCATGA T^CATGA
Sau3AI 1093 GATC ^GATC
SwaI 315 ATTTAAAT ATTT^AAAT
Tsp45I 146 GTGAC ^GT[GC]AC
XhoI 820 CTCGAG C^TCGAG
XhoII 1092 GGATCC [GA]^GATC[CT]
XmnI 606 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 1093 GATCC GGATC(4/5)
1092 GGATC
BsiYI 818 CCCTCGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
819 CCTCGAGCGGG
BslI 818 CCCTCGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
819 CCTCGAGCGGG
BsmAI 101 GAGAC GTCTC(1/5)
829 GAGAC
DdeI 515 CTCAG C^T[ACGT]AG
805 CTCAG
DraII 438 AGGGCCT [GA]G^G[ACGT]CC[CT]
518 AGGCCCT
EcoO109I 438 AGGGCCT [GA]G^G[ACGT]CC[CT]
518 AGGCCCT
EcoT14I 935 CCAAGG C^C[AT][AT]GG
944 CCATGG
MaeII 446 ACGT A^CGT
893 ACGT
MslI 477 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1022 CATCAGAATG
MwoI 511 GCAGCTCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
658 GCAAACACTGC
PleI 573 GAGTC GAGTC(4/5)
905 GAGTC
SfcI 347 CTGCAG C^T[GA][CT]AG
590 CTACAG
StyI 935 CCAAGG C^C[AT][AT]GG
944 CCATGG
TaqI 243 TCGA T^CGA
821 TCGA
TspRI 593 CAGTG CA[GC]TG[ACGT][ACGT]^
663 CACTG
TthHB8I 243 TCGA T^CGA
821 TCGA
XcmI 935 CCAAGGTGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
977 CCAAAGACATATTGG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsaJI BssKI BstF5I BstNI
Cfr13I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MboII MnlI
MseI MvaI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI SmlI TfiI
Tru9I TseI Tsp509I