Restriction map for
pF1KE2464
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BanI
BbeI BbvCI BclI BfrI BglI
BlnI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsaI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 716 CGCG CG^CG
AocI 621 CCTGAGG CC^T[ACGT]AGG
Asp700I 369 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1381 CTCGAG C^[CT]CG[GA]G
AviII 1703 TGCGCA TGC^GCA
BalI 251 TGGCCA TGG^CCA
BbrPI 1072 CACGTG CAC^GTG
BciVI 489 GGATAC GTATCC(6/5)
BglII 1212 AGATCT A^GATCT
BsaAI 1072 CACGTG [CT]AC^GT[GA]
BsgI 528 CTGCAC GTGCAG(16/14)
BsoBI 1381 CTCGAG C^[CT]CG[GA]G
BsrDI 61 CATTGC GCAATG(2/0)
BsrFI 2123 GCCGGC [GA]^CCGG[CT]
BssHII 715 GCGCGC G^CGCGC
BssSI 322 CACGAG CACGAG(-5/-1)
BstUI 716 CGCG CG^CG
BstXI 428 CCAAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 621 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2123 GCCGGC [GA]^CCGG[CT]
CpoI 2105 CGGACCG CG^G[AT]CCG
DraII 1927 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 1280 GACTACACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 824 CCATGG C^C[GA][CT]GG
EaeI 251 TGGCCA [CT]^GGCC[GA]
Eco81I 621 CCTGAGG CC^T[ACGT]AGG
EcoNI 399 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1927 GGGACCC [GA]G^G[ACGT]CC[CT]
FspI 1703 TGCGCA TGC^GCA
HaeII 242 AGCGCC [GA]GCGC^[CT]
HindIII 1109 AAGCTT A^AGCTT
MluNI 251 TGGCCA TGG^CCA
MscI 251 TGGCCA TGG^CCA
MslI 1916 CACGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 716 CGCG CG^CG
NaeI 2123 GCCGGC GCC^GGC
NcoI 824 CCATGG C^CATGG
NgoMIV 2123 GCCGGC G^CCGGC
PaeR7I 1381 CTCGAG C^TCGAG
PflMI 300 CCACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1072 CACGTG CAC^GTG
PmlI 1072 CACGTG CAC^GTG
PpuMI 1927 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 142 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2105 CGGACCG CG^G[AT]CCG
SacI 760 GAGCTC GAGCT^C
StuI 1400 AGGCCT AGG^CCT
Van91I 300 CCACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1381 CTCGAG C^TCGAG
XmnI 369 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1071 ACACGT A^C[GA][CT]GT
2219 ACGTGT
BanII 760 GAGCTC G[GA]GC[CT]^C
1022 GAGCCC
BbsI 1255 GAAGAC GAAGAC(2/6)
1455 GAAGAC
BcgI 1050 CGAGGAGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1706 GCAGCCGGATCG
BfaI 1492 CTAG C^TAG
2255 CTAG
BpuAI 1255 GAAGAC GAAGAC(2/6)
1455 GAAGAC
BsmI 195 GAATGC GAATGC(1/-1)
1309 GCATTC
BsrBI 2268 CCGCTC CCGCTC(-3/-3)
1663 GAGCGG
FauI 920 GCGGG CCCGC(4/6)
1251 GCGGG
MaeI 1492 CTAG C^TAG
2255 CTAG
NspI 893 GCATGC [GA]CATG^[CT]
2177 GCATGC
SphI 893 GCATGC GCATG^C
2177 GCATGC
Tsp509I 1185 AATT ^AATT
2072 AATT
XcmI 254 CCAAAAGGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
467 CCAAGCAGGTCATGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII