Restriction map for
pF1KE2603
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BbrPI BcgI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MamI MluI MroI
MvnI NdeI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 304 GAATTC [GA]^AATT[CT]
AcyI 2830 GGCGCC G[GA]^CG[CT]C
AflIII 1514 ACATGT A^C[GA][CT]GT
Alw44I 2429 GTGCAC G^TGCAC
AocI 559 CCTCAGG CC^T[ACGT]AGG
ApaLI 2429 GTGCAC G^TGCAC
ApoI 304 GAATTC [GA]^AATT[CT]
Asp700I 1579 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2695 GGATCC G^GATCC
BanII 1953 GAGCCC G[GA]GC[CT]^C
BbeI 2830 GGCGCC GGCGC^C
BbvCI 637 CCTCAGC CCTCAGC(-5/-2)
BglII 1873 AGATCT A^GATCT
BlpI 2238 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2238 GCTCAGC GC^T[ACGT]AGC
BsaHI 2830 GGCGCC G[GA]^CG[CT]C
BspLU11I 1514 ACATGT A^CATGT
BsrBI 2286 CCGCTC CCGCTC(-3/-3)
BstAPI 2725 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2884 GGTGACC G^GT[ACGT]ACC
BstPI 2884 GGTGACC G^GT[ACGT]ACC
Bsu36I 559 CCTCAGG CC^T[ACGT]AGG
CelII 2238 GCTCAGC GC^T[ACGT]AGC
DrdI 2028 GACTGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 559 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2884 GGTGACC G^GT[ACGT]ACC
EcoRI 304 GAATTC G^AATTC
EcoT22I 23 ATGCAT ATGCA^T
Hin1I 2830 GGCGCC G[GA]^CG[CT]C
KasI 2830 GGCGCC G^GCGCC
MfeI 2420 CAATTG C^AATTG
MunI 2420 CAATTG C^AATTG
NarI 2830 GGCGCC GG^CGCC
NcoI 884 CCATGG C^CATGG
NheI 663 GCTAGC G^CTAGC
NsiI 23 ATGCAT ATGCA^T
PflMI 2901 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 2722 CCTGCAGG CCTGCA^GG
SfiI 2851 GGCCTCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2830 GGCGCC GGC^GCC
SmlI 2052 CTTGAG C^T[CT][GA]AG
Sse8387I 2722 CCTGCAGG CCTGCA^GG
Van91I 2901 CCAGACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1579 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
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BanI 750 GGCACC G^G[CT][GA]CC
2830 GGCGCC
BbsI 735 GAAGAC GAAGAC(2/6)
2739 GAAGAC
BglI 2163 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2852 GCCTCAATGGC
BpuAI 735 GAAGAC GAAGAC(2/6)
2739 GAAGAC
BsaI 293 GGTCTC GGTCTC(1/5)
2583 GGTCTC
BsrDI 75 CATTGC GCAATG(2/0)
1806 CATTGC
BsrFI 2413 GCCGGC [GA]^CCGG[CT]
2799 GCCGGC
CfoI 2818 GCGC GCG^C
2831 GCGC
Cfr10I 2413 GCCGGC [GA]^CCGG[CT]
2799 GCCGGC
DraII 1643 AGGTCCC [GA]G^G[ACGT]CC[CT]
2778 GGGTCCC
DsaI 884 CCATGG C^C[GA][CT]GG
2303 CCACGG
EarI 313 GAAGAG CTCTTC(1/4)
691 GAAGAG
EcoO109I 1643 AGGTCCC [GA]G^G[ACGT]CC[CT]
2778 GGGTCCC
HaeII 2817 AGCGCC [GA]GCGC^[CT]
2830 GGCGCC
HhaI 2818 GCGC GCG^C
2831 GCGC
HinP1I 2818 GCGC G^CGC
2831 GCGC
Ksp632I 313 GAAGAG CTCTTC(1/4)
691 GAAGAG
MslI 433 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2154 CACACCCATG
NaeI 2413 GCCGGC GCC^GGC
2799 GCCGGC
NgoMIV 2413 GCCGGC G^CCGGC
2799 GCCGGC
PpuMI 1643 AGGTCCC [GA]G^G[AT]CC[CT]
2778 GGGTCCC
PshAI 7 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2050 GACTTGAGTC
SspI 489 AATATT AAT^ATT
2518 AATATT
StuI 95 AGGCCT AGG^CCT
1765 AGGCCT
TaqI 398 TCGA T^CGA
2702 TCGA
TthHB8I 398 TCGA T^CGA
2702 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EcoNI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII