Restriction map for
pF1KE2641
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccI AccIII AciI AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV EcoT22I FauI
FseI FspI Hin1I HpaI KasI
KspI MaeII MfeI MluI MroI
MslI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TthHB8I Van91I
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 462 GGTACC G^GTACC
AccII 1246 CGCG CG^CG
AlwI 372 GGATC GGATC(4/5)
Aor51HI 806 AGCGCT AGC^GCT
Asp718I 462 GGTACC G^GTACC
AspHI 412 GAGCAC G[AT]GC[AT]^C
AspI 918 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 291 GGACC G^G[AT]CC
BalI 246 TGGCCA TGG^CCA
BbsI 704 GAAGAC GAAGAC(2/6)
BcnI 78 CCGGG CC^[GC]GG
BfaI 16 CTAG C^TAG
BpuAI 704 GAAGAC GAAGAC(2/6)
BsaBI 204 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 73 GGTCTC GGTCTC(1/5)
BseRI 19 GAGGAG GAGGAG(10/8)
BsgI 908 CTGCAC GTGCAG(16/14)
BsiHKAI 412 GAGCAC G[AT]GC[AT]^C
BspMI 47 ACCTGC ACCTGC(4/8)
BsrFI 752 GCCGGT [GA]^CCGG[CT]
BstUI 1246 CGCG CG^CG
BstXI 62 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 372 GGATCT [GA]^GATC[CT]
CfoI 807 GCGC GCG^C
Cfr10I 752 GCCGGT [GA]^CCGG[CT]
DraII 1295 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 1129 CCATGG C^C[GA][CT]GG
EaeI 246 TGGCCA [CT]^GGCC[GA]
EarI 1228 GAAGAG CTCTTC(1/4)
Eco47III 806 AGCGCT AGC^GCT
EcoO109I 1295 GGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 806 AGCGCT [GA]GCGC^[CT]
HgaI 740 GACGC GACGC(5/10)
HhaI 807 GCGC GCG^C
HinP1I 807 GCGC G^CGC
HindIII 556 AAGCTT A^AGCTT
HphI 254 TCACC GGTGA(8/7)
KpnI 462 GGTACC GGTAC^C
Ksp632I 1228 GAAGAG CTCTTC(1/4)
MaeI 16 CTAG C^TAG
MamI 204 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 372 GGATCT [GA]^GATC[CT]
MluNI 246 TGGCCA TGG^CCA
MscI 246 TGGCCA TGG^CCA
MspA1I 848 CAGCTG C[AC]G^C[GT]G
MvnI 1246 CGCG CG^CG
NciI 78 CCGGG CC^[GC]GG
NcoI 1129 CCATGG C^CATGG
PflFI 918 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1038 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 848 CAGCTG CAG^CTG
SapI 1228 GAAGAGC GCTCTTC(1/4)
SfaNI 181 GCATC GCATC(5/9)
Tth111I 918 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 15 TCTAGA T^CTAGA
XhoII 372 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 340 GAATTC [GA]^AATT[CT]
987 GAATTC
AflIII 912 ACATGT A^C[GA][CT]GT
1167 ACATGT
AocI 87 CCTGAGG CC^T[ACGT]AGG
440 CCTCAGG
ApoI 340 GAATTC [GA]^AATT[CT]
987 GAATTC
BanI 462 GGTACC G^G[CT][GA]CC
931 GGCACC
BclI 2 TGATCA T^GATCA
594 TGATCA
BmyI 412 GAGCAC G[AGT]GC[ACT]^C
930 GGGCAC
BsaJI 782 CCTTGG C^C[ACGT][ACGT]GG
1129 CCATGG
BsmFI 958 GGGAC GGGAC(10/14)
1091 GTCCC
BsmI 429 GAATGC GAATGC(1/-1)
1286 GAATGC
Bsp1286I 412 GAGCAC G[AGT]GC[ACT]^C
930 GGGCAC
BspHI 351 TCATGA T^CATGA
561 TCATGA
BspLU11I 912 ACATGT A^CATGT
1167 ACATGT
Bsu36I 87 CCTGAGG CC^T[ACGT]AGG
440 CCTCAGG
Cac8I 743 GCTCGC GC[ACGT]^[ACGT]GC
1247 GCGAGC
Eco81I 87 CCTGAGG CC^T[ACGT]AGG
440 CCTCAGG
EcoNI 466 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
694 CCTCCAAGAGG
EcoRI 340 GAATTC G^AATTC
987 GAATTC
EcoT14I 782 CCTTGG C^C[AT][AT]GG
1129 CCATGG
FbaI 2 TGATCA T^GATCA
594 TGATCA
HapII 78 CCGG C^CGG
753 CCGG
HincII 1017 GTTGAC GT[CT]^[GA]AC
1171 GTCAAC
HindII 1017 GTTGAC GT[CT]^[GA]AC
1171 GTCAAC
HpaII 78 CCGG C^CGG
753 CCGG
MspI 78 CCGG C^CGG
753 CCGG
NspI 912 ACATGT [GA]CATG^[CT]
1167 ACATGT
PstI 49 CTGCAG CTGCA^G
821 CTGCAG
RcaI 351 TCATGA T^CATGA
561 TCATGA
SfcI 49 CTGCAG C^T[GA][CT]AG
821 CTGCAG
SmlI 34 CTCAAG C^T[CT][GA]AG
802 CTCAAG
StyI 782 CCTTGG C^C[AT][AT]GG
1129 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI BbvI BpmI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI