Restriction map for
pF1KE2636
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DrdI DsaI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 391 GACGTC GACGT^C
AccI 953 GTCTAC GT^[AC][GT]AC
AcyI 391 GACGTC G[GA]^CG[CT]C
AgeI 90 ACCGGT A^CCGGT
AhdI 421 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 735 GGATC GGATC(4/5)
ApaI 935 GGGCCC GGGCC^C
AspEI 421 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 319 CCCGAG C^[CT]CG[GA]G
BalI 929 TGGCCA TGG^CCA
BbsI 409 GAAGAC GAAGAC(2/6)
BcgI 789 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 606 TGATCA T^GATCA
BcnI 630 CCGGG CC^[GC]GG
BglI 823 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 409 GAAGAC GAAGAC(2/6)
BsaHI 391 GACGTC G[GA]^CG[CT]C
BsaWI 90 ACCGGT [AT]^CCGG[AT]
BseRI 790 GAGGAG GAGGAG(10/8)
BsmAI 238 GAGAC GTCTC(1/5)
BsmFI 769 GTCCC GGGAC(10/14)
BsoBI 319 CCCGAG C^[CT]CG[GA]G
BspMI 476 GCAGGT ACCTGC(4/8)
BsrFI 90 ACCGGT [GA]^CCGG[CT]
BstAPI 364 GCAAGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 90 ACCGGT [GA]^CCGG[CT]
DraI 1036 TTTAAA TTT^AAA
DraII 934 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 916 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 929 TGGCCA [CT]^GGCC[GA]
Eam1105I 421 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 934 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 620 GATATC GAT^ATC
FauI 373 GCGGG CCCGC(4/6)
FbaI 606 TGATCA T^GATCA
HgaI 589 GACGC GACGC(5/10)
Hin1I 391 GACGTC G[GA]^CG[CT]C
MluNI 929 TGGCCA TGG^CCA
MscI 929 TGGCCA TGG^CCA
MslI 739 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 384 CCGCTG C[AC]G^C[GT]G
NciI 630 CCGGG CC^[GC]GG
PinAI 90 ACCGGT A^CCGGT
PleI 677 GAGTC GAGTC(4/5)
PshAI 240 GACTACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 459 CTACAG C^T[GA][CT]AG
SgrAI 89 CACCGGTG C[GA]^CCGG[CT]G
TaqI 788 TCGA T^CGA
TfiI 692 GATTC G^A[AT]TC
TthHB8I 788 TCGA T^CGA
XcmI 939 CCAGTGTGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 399 GTAC GT^AC
480 GTAC
AluI 356 AGCT AG^CT
551 AGCT
BpmI 542 CTCCAG CTGGAG(16/14)
847 CTCCAG
CfoI 58 GCGC GCG^C
912 GCGC
DdeI 200 CTAAG C^T[ACGT]AG
503 CTCAG
DpnII 607 GATC ^GATC
736 GATC
HapII 91 CCGG C^CGG
630 CCGG
HhaI 58 GCGC GCG^C
912 GCGC
HinP1I 58 GCGC G^CGC
912 GCGC
HinfI 677 GAGTC G^A[ACGT]TC
692 GATTC
HpaII 91 CCGG C^CGG
630 CCGG
MboI 607 GATC ^GATC
736 GATC
MboII 409 GAAGA GAAGA(8/7)
568 GAAGA
MseI 43 TTAA T^TAA
1037 TTAA
MspI 91 CCGG C^CGG
630 CCGG
NdeII 607 GATC ^GATC
736 GATC
RsaI 399 GTAC GT^AC
480 GTAC
Sau3AI 607 GATC ^GATC
736 GATC
Tru9I 43 TTAA T^TAA
1037 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AcsI ApoI BanII BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I EcoRII Fnu4HI FokI HaeIII
HphI ItaI MaeII MaeIII MnlI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI SfaNI TseI Tsp45I Tsp509I
TspRI