Restriction map for
pF1KE2467
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssKI BssSI
Bst1107I BstAPI BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI Eam1105I
Eco47III EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI MvaI
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI ScrFI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 280 GTAGAC GT^[AC][GT]AC
AciI 270 GCGG CCGC(-3/-1)
AfaI 196 GTAC GT^AC
AlwI 411 GGATC GGATC(4/5)
AocI 663 CCTGAGG CC^T[ACGT]AGG
BalI 423 TGGCCA TGG^CCA
BanII 597 GAGCCC G[GA]GC[CT]^C
BfaI 16 CTAG C^TAG
BsaAI 277 TACGTA [CT]AC^GT[GA]
BsaI 327 GAGACC GGTCTC(1/5)
BsiEI 673 CGGCCG CG[GA][CT]^CG
BsmFI 626 GGGAC GGGAC(10/14)
BsrDI 77 GCAATG GCAATG(2/0)
BsrFI 675 GCCGGC [GA]^CCGG[CT]
BsrI 72 ACTGG ACTGG(1/-1)
BstYI 411 GGATCT [GA]^GATC[CT]
Bsu36I 663 CCTGAGG CC^T[ACGT]AGG
Cac8I 675 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 675 GCCGGC [GA]^CCGG[CT]
EagI 673 CGGCCG C^GGCCG
EclXI 673 CGGCCG C^GGCCG
Eco52I 673 CGGCCG C^GGCCG
Eco81I 663 CCTGAGG CC^T[ACGT]AGG
HapII 676 CCGG C^CGG
HpaII 676 CCGG C^CGG
HphI 142 GGTGA GGTGA(8/7)
MaeI 16 CTAG C^TAG
MaeII 278 ACGT A^CGT
MflI 411 GGATCT [GA]^GATC[CT]
MluNI 423 TGGCCA TGG^CCA
MscI 423 TGGCCA TGG^CCA
MslI 648 CATTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 676 CCGG C^CGG
NaeI 675 GCCGGC GCC^GGC
NgoMIV 675 GCCGGC G^CCGGC
NlaIII 581 CATG CATG^
NspI 580 GCATGT [GA]CATG^[CT]
PleI 557 GAGTC GAGTC(4/5)
RsaI 196 GTAC GT^AC
SfaNI 4 GATGC GCATC(5/9)
SfcI 302 CTACAG C^T[GA][CT]AG
SmlI 125 CTCAAG C^T[CT][GA]AG
SnaBI 277 TACGTA TAC^GTA
XcmI 470 CCAAAATCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 411 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 21 AAATTC [GA]^AATT[CT]
520 AAATTT
AluI 316 AGCT AG^CT
444 AGCT
ApoI 21 AAATTC [GA]^AATT[CT]
520 AAATTT
BbvI 577 GCAGC GCAGC(8/12)
490 GCTGC
BmyI 597 GAGCCC G[AGT]GC[ACT]^C
668 GGGCAC
BsmAI 327 GAGAC GTCTC(1/5)
101 GTCTC
Bsp1286I 597 GAGCCC G[AGT]GC[ACT]^C
668 GGGCAC
DdeI 238 CTTAG C^T[ACGT]AG
664 CTGAG
EaeI 423 TGGCCA [CT]^GGCC[GA]
673 CGGCCG
EarI 85 CTCTTC CTCTTC(1/4)
324 GAAGAG
HaeIII 424 GGCC GG^CC
674 GGCC
HincII 355 GTTGAC GT[CT]^[GA]AC
559 GTCAAC
HindII 355 GTTGAC GT[CT]^[GA]AC
559 GTCAAC
Ksp632I 85 CTCTTC CTCTTC(1/4)
324 GAAGAG
MseI 407 TTAA T^TAA
485 TTAA
MwoI 19 GCAAATTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
670 GCACGGCCGGC
TfiI 294 GAATC G^A[AT]TC
529 GAATC
Tru9I 407 TTAA T^TAA
485 TTAA
TseI 490 GCTGC G^C[AT]GC
577 GCAGC
Tsp45I 117 GTCAC ^GT[GC]AC
143 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BstF5I DpnII Fnu4HI FokI HinfI
ItaI MaeIII MboI MboII MnlI
NdeII NlaIV Sau3AI Tsp509I TspRI