Restriction map for
pF1KE4045
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BciVI
BclI BcnI BfaI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tsp45I
Tsp509I Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AflII 361 CTTAAG C^TTAAG
Asp700I 232 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 388 GAGCCC G[GA]GC[CT]^C
BcgI 84 GCAGTGCGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 361 CTTAAG C^TTAAG
BmyI 388 GAGCCC G[AGT]GC[ACT]^C
BsaI 714 GGTCTC GGTCTC(1/5)
BsgI 332 CTGCAC GTGCAG(16/14)
BsiEI 90 CGATCG CG[GA][CT]^CG
BsiYI 178 CCAGAATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 178 CCAGAATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 354 GGGAC GGGAC(10/14)
Bsp1286I 388 GAGCCC G[AGT]GC[ACT]^C
BstAPI 36 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
HindIII 198 AAGCTT A^AGCTT
HinfI 276 GATTC G^A[ACGT]TC
MaeII 797 ACGT A^CGT
MspA1I 401 CAGCTG C[AC]G^C[GT]G
MwoI 36 GCAGCATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 178 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 90 CGATCG CGAT^CG
PvuII 401 CAGCTG CAG^CTG
SmlI 361 CTTAAG C^T[CT][GA]AG
TaqI 209 TCGA T^CGA
TfiI 276 GATTC G^A[AT]TC
TthHB8I 209 TCGA T^CGA
Van91I 178 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 232 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 45 GCGG CCGC(-3/-1)
352 GCGG
BsaJI 78 CCGTGG C^C[ACGT][ACGT]GG
116 CCACGG
BsmAI 99 GAGAC GTCTC(1/5)
715 GTCTC
BsrI 734 ACTGG ACTGG(1/-1)
554 CCAGT
BssKI 680 CCTGG ^CC[ACGT]GG
768 CCTGG
BstNI 680 CCTGG CC^[AT]GG
768 CCTGG
BstYI 268 GGATCT [GA]^GATC[CT]
550 AGATCC
Cac8I 45 GCGGGC GC[ACGT]^[ACGT]GC
214 GCTTGC
Cfr13I 27 GGCCC G^G[ACGT]CC
47 GGGCC
DdeI 532 CTCAG C^T[ACGT]AG
547 CTTAG
DsaI 78 CCGTGG C^C[GA][CT]GG
116 CCACGG
EcoRII 680 CCTGG ^CC[AT]GG
768 CCTGG
FauI 45 GCGGG CCCGC(4/6)
352 GCGGG
HaeIII 27 GGCC GG^CC
48 GGCC
MflI 268 GGATCT [GA]^GATC[CT]
550 AGATCC
MvaI 680 CCTGG CC^[AT]GG
768 CCTGG
Sau96I 27 GGCCC G^G[ACGT]CC
47 GGGCC
ScrFI 680 CCTGG CC^[ACGT]GG
768 CCTGG
SfaNI 211 GATGC GCATC(5/9)
39 GCATC
SspI 135 AATATT AAT^ATT
591 AATATT
XhoII 268 GGATCT [GA]^GATC[CT]
550 AGATCC
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# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AfaI AluI AlwI AlwNI BbsI
BbvI BpuAI DpnII Fnu4HI HphI
ItaI MboI MboII MnlI MseI
NdeII NlaIII RsaI Sau3AI Tru9I
TseI TspRI