Restriction map for
pF1KE9399
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# NO CUT ENZYMES ( 37 enzymes) #
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Acc65I AclI AgeI AscI AseI
AsnI Asp718I BsiWI BspHI BssHII
BstBI DraI HindIII HpaI KpnI
MluI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI SalI
SfuI SnaBI SpeI SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AflII 7705 CTTAAG C^TTAAG
AhdI 2929 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2929 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 6360 CCTAGG C^CTAGG
BcgI 6225 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 911 TGATCA T^GATCA
BfrI 7705 CTTAAG C^TTAAG
BlnI 6360 CCTAGG C^CTAGG
BsaBI 7797 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1797 GAGACG CGTCTC(1/5)
BsmI 4687 GCATTC GAATGC(1/-1)
BspDI 2416 ATCGAT AT^CGAT
BsrDI 7934 GCAATG GCAATG(2/0)
BssSI 3637 CACGAG CACGAG(-5/-1)
Bst1107I 3001 GTATAC GTA^TAC
BstEII 2130 GGTCACC G^GT[ACGT]ACC
BstPI 2130 GGTCACC G^GT[ACGT]ACC
BstZ17I 3001 GTATAC GTA^TAC
ClaI 2416 ATCGAT AT^CGAT
Eam1105I 2929 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2130 GGTCACC G^GT[ACGT]ACC
EcoRI 5336 GAATTC G^AATTC
EcoRV 3259 GATATC GAT^ATC
FbaI 911 TGATCA T^GATCA
MamI 7797 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 952 CAATTG C^AATTG
MunI 952 CAATTG C^AATTG
SexAI 3727 ACCTGGT A^CC[AT]GGT
SfiI 5351 GGCCATGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 78 CGCCGGCG C[GA]^CCGG[CT]G
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# TWO CUT ENZYMES ( 25 enzymes) #
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AatII 3943 GACGTC GACGT^C
7713 GACGTC
AccI 3001 GTATAC GT^[AC][GT]AC
7735 GTCTAC
AcsI 3748 AAATTC [GA]^AATT[CT]
5336 GAATTC
ApoI 3748 AAATTC [GA]^AATT[CT]
5336 GAATTC
Asp700I 2370 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5034 GAAAGTCTTC
AspI 281 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2900 GACACTGTC
BalI 4374 TGGCCA TGG^CCA
7685 TGGCCA
BbrPI 1751 CACGTG CAC^GTG
7993 CACGTG
BciVI 2721 GGATAC GTATCC(6/5)
6924 GGATAC
BglII 1979 AGATCT A^GATCT
2504 AGATCT
BspLU11I 2607 ACATGT A^CATGT
7565 ACATGT
CpoI 4170 CGGACCG CG^G[AT]CCG
4454 CGGACCG
EcoT22I 3818 ATGCAT ATGCA^T
7927 ATGCAT
MluNI 4374 TGGCCA TGG^CCA
7685 TGGCCA
MscI 4374 TGGCCA TGG^CCA
7685 TGGCCA
NsiI 3818 ATGCAT ATGCA^T
7927 ATGCAT
PflFI 281 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2900 GACACTGTC
PmaCI 1751 CACGTG CAC^GTG
7993 CACGTG
PmlI 1751 CACGTG CAC^GTG
7993 CACGTG
RsrII 4170 CGGACCG CG^G[AT]CCG
4454 CGGACCG
SbfI 689 CCTGCAGG CCTGCA^GG
2220 CCTGCAGG
ScaI 4541 AGTACT AGT^ACT
7808 AGTACT
Sse8387I 689 CCTGCAGG CCTGCA^GG
2220 CCTGCAGG
Tth111I 281 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2900 GACACTGTC
XmnI 2370 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5034 GAAAGTCTTC
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# 3 OR MORE CUT ENZYMES ( 166 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AspHI
AvaI AvaII AviII BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspEI BspMI BsrBI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FseI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI