Restriction map for
pF1KE4271
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaHI
BsiWI BspDI BspLU11I BsrBI BsrFI
BssHII BssSI BstUI Bsu36I Cfr10I
ClaI DraII DrdI DsaI Eam1105I
Eco47III Eco81I EcoO109I FbaI FseI
FspI Hin1I HindIII KasI KpnI
KspI MluI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NruI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI SacII SalI
SapI SbfI ScaI SfoI SgrAI
SmaI SpeI SphI Sse8387I StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 2003 TCCGGA T^CCGGA
Asp700I 411 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 800 CCCGAG C^[CT]CG[GA]G
BalI 2510 TGGCCA TGG^CCA
BamHI 3123 GGATCC G^GATCC
BanI 2588 GGCACC G^G[CT][GA]CC
BbsI 518 GTCTTC GAAGAC(2/6)
BglI 169 GCCGCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 518 GTCTTC GAAGAC(2/6)
BsaI 264 GAGACC GGTCTC(1/5)
BseAI 2003 TCCGGA T^CCGGA
BsmBI 1716 GAGACG CGTCTC(1/5)
BsoBI 800 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2550 TGTACA T^GTACA
BspEI 2003 TCCGGA T^CCGGA
BsrDI 2964 CATTGC GCAATG(2/0)
BsrGI 2550 TGTACA T^GTACA
BstBI 555 TTCGAA TT^CGAA
BstEII 1737 GGTGACC G^GT[ACGT]ACC
BstPI 1737 GGTGACC G^GT[ACGT]ACC
BstXI 2293 CCAACTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 2005 CGGACCG CG^G[AT]CCG
EcoO65I 1737 GGTGACC G^GT[ACGT]ACC
EcoRV 511 GATATC GAT^ATC
HgaI 19 GCGTC GACGC(5/10)
HpaI 1477 GTTAAC GTT^AAC
MfeI 2783 CAATTG C^AATTG
MluNI 2510 TGGCCA TGG^CCA
MroI 2003 TCCGGA T^CCGGA
MscI 2510 TGGCCA TGG^CCA
MunI 2783 CAATTG C^AATTG
NspV 555 TTCGAA TT^CGAA
PvuII 220 CAGCTG CAG^CTG
RsrII 2005 CGGACCG CG^G[AT]CCG
SfiI 168 GGCCGCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 555 TTCGAA TT^CGAA
SnaBI 1844 TACGTA TAC^GTA
SspBI 2550 TGTACA T^GTACA
SspI 811 AATATT AAT^ATT
XbaI 2430 TCTAGA T^CTAGA
XcmI 135 CCAGGTGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 411 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AlwNI 47 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
155 CAGCGGCTG
BanII 805 GAGCTC G[GA]GC[CT]^C
2706 GAGCTC
BlpI 44 GCTCAGC GC^T[ACGT]AGC
2521 GCTAAGC
BpmI 39 CTCCAG CTGGAG(16/14)
3236 CTGGAG
Bpu1102I 44 GCTCAGC GC^T[ACGT]AGC
2521 GCTAAGC
BsaAI 232 CACGTA [CT]AC^GT[GA]
1844 TACGTA
BsaBI 1027 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2733 GATTACCATC
BsaWI 2003 TCCGGA [AT]^CCGG[AT]
2683 ACCGGA
BsiEI 167 CGGCCG CG[GA][CT]^CG
176 CGGCCG
BsmI 534 GAATGC GAATGC(1/-1)
2180 GAATGC
BspHI 627 TCATGA T^CATGA
2949 TCATGA
BspMI 1968 ACCTGC ACCTGC(4/8)
3353 ACCTGC
Bst1107I 851 GTATAC GTA^TAC
2288 GTATAC
BstYI 2174 AGATCC [GA]^GATC[CT]
3123 GGATCC
BstZ17I 851 GTATAC GTA^TAC
2288 GTATAC
CelII 44 GCTCAGC GC^T[ACGT]AGC
2521 GCTAAGC
DraI 1573 TTTAAA TTT^AAA
2107 TTTAAA
DraIII 2590 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3067 CACCAGGTG
EagI 167 CGGCCG C^GGCCG
176 CGGCCG
EclXI 167 CGGCCG C^GGCCG
176 CGGCCG
Eco52I 167 CGGCCG C^GGCCG
176 CGGCCG
EcoNI 1183 CCTGCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2508 CCTGGCCAAGG
EcoT14I 2513 CCAAGG C^C[AT][AT]GG
2909 CCTTGG
EcoT22I 2182 ATGCAT ATGCA^T
3337 ATGCAT
FauI 205 CCCGC CCCGC(4/6)
213 CCCGC
MamI 1027 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2733 GATTACCATC
MflI 2174 AGATCC [GA]^GATC[CT]
3123 GGATCC
NotI 166 GCGGCCGC GC^GGCCGC
175 GCGGCCGC
NsiI 2182 ATGCAT ATGCA^T
3337 ATGCAT
RcaI 627 TCATGA T^CATGA
2949 TCATGA
SacI 805 GAGCTC GAGCT^C
2706 GAGCTC
SexAI 134 ACCAGGT A^CC[AT]GGT
3068 ACCAGGT
SmlI 2980 CTTGAG C^T[CT][GA]AG
3310 CTCAAG
StyI 2513 CCAAGG C^C[AT][AT]GG
2909 CCTTGG
XhoII 2174 AGATCC [GA]^GATC[CT]
3123 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BbvI BcnI BfaI BmyI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRI EcoRII Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PshBI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I