Restriction map for
pF1KE3268
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccII AflII AflIII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspHI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BcgI BcnI
BfaI BfrI BglI BglII BlnI
BsiHKAI BsiWI BsmBI BsoBI BspHI
BspLU11I BspMI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Cac8I Cfr13I CpoI
DraII DraIII DrdI Eco47III EcoO109I
EcoO65I EcoT22I FseI FspI HincII
HindII HpaI KasI KpnI KspI
MaeI MfeI MluI MluNI MscI
MunI MvnI MwoI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SspI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 1433 GTCTAC GT^[AC][GT]AC
AccIII 1909 TCCGGA T^CCGGA
AclI 1622 AACGTT AA^CGTT
AcyI 31 GACGCC G[GA]^CG[CT]C
AhdI 1442 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1030 CCTCAGG CC^T[ACGT]AGG
Asp700I 1811 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1442 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1512 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 909 GGTGCC G^G[CT][GA]CC
BanII 1153 GAGCCC G[GA]GC[CT]^C
BbvCI 580 CCTCAGC CCTCAGC(-5/-2)
BciVI 1268 GGATAC GTATCC(6/5)
BpmI 998 CTCCAG CTGGAG(16/14)
BsaAI 1640 TACGTA [CT]AC^GT[GA]
BsaHI 31 GACGCC G[GA]^CG[CT]C
BsaI 1063 GAGACC GGTCTC(1/5)
BsaJI 932 CCATGG C^C[ACGT][ACGT]GG
BsaWI 1909 TCCGGA [AT]^CCGG[AT]
BseAI 1909 TCCGGA T^CCGGA
BsgI 950 GTGCAG GTGCAG(16/14)
BsiEI 646 CGGCCG CG[GA][CT]^CG
Bsp1407I 1376 TGTACA T^GTACA
BspDI 1718 ATCGAT AT^CGAT
BspEI 1909 TCCGGA T^CCGGA
BsrFI 648 GCCGGT [GA]^CCGG[CT]
BsrGI 1376 TGTACA T^GTACA
Bsu36I 1030 CCTCAGG CC^T[ACGT]AGG
Cfr10I 648 GCCGGT [GA]^CCGG[CT]
ClaI 1718 ATCGAT AT^CGAT
DraI 1453 TTTAAA TTT^AAA
DsaI 932 CCATGG C^C[GA][CT]GG
EaeI 646 CGGCCG [CT]^GGCC[GA]
EagI 646 CGGCCG C^GGCCG
Eam1105I 1442 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 646 CGGCCG C^GGCCG
Eco52I 646 CGGCCG C^GGCCG
Eco81I 1030 CCTCAGG CC^T[ACGT]AGG
EcoNI 994 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 958 GATATC GAT^ATC
EcoT14I 932 CCATGG C^C[AT][AT]GG
FauI 1686 GCGGG CCCGC(4/6)
HgaI 31 GACGC GACGC(5/10)
Hin1I 31 GACGCC G[GA]^CG[CT]C
HindIII 266 AAGCTT A^AGCTT
MroI 1909 TCCGGA T^CCGGA
NcoI 932 CCATGG C^CATGG
NspI 1340 GCATGT [GA]CATG^[CT]
PflFI 1512 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1564 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 352 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1622 AACGTT AA^CGTT
PvuII 706 CAGCTG CAG^CTG
SexAI 1228 ACCAGGT A^CC[AT]GGT
SfaNI 877 GCATC GCATC(5/9)
SnaBI 1640 TACGTA TAC^GTA
SspBI 1376 TGTACA T^GTACA
StyI 932 CCATGG C^C[AT][AT]GG
Tth111I 1512 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1564 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1811 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 1377 GTAC GT^AC
1427 GTAC
AlwNI 80 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
812 CAGAATCTG
BbsI 349 GAAGAC GAAGAC(2/6)
982 GAAGAC
BlpI 46 GCTGAGC GC^T[ACGT]AGC
1692 GCTGAGC
BmyI 500 GGGCAC G[AGT]GC[ACT]^C
1153 GAGCCC
Bpu1102I 46 GCTGAGC GC^T[ACGT]AGC
1692 GCTGAGC
BpuAI 349 GAAGAC GAAGAC(2/6)
982 GAAGAC
BsmAI 600 GAGAC GTCTC(1/5)
1063 GAGAC
BsmFI 1440 GGGAC GGGAC(10/14)
1344 GTCCC
Bsp1286I 500 GGGCAC G[AGT]GC[ACT]^C
1153 GAGCCC
BsrDI 1307 GCAATG GCAATG(2/0)
1358 GCAATG
BstXI 1700 CCAGTTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1751 CCATCAGCTTGG
CelII 46 GCTGAGC GC^T[ACGT]AGC
1692 GCTGAGC
CfoI 1659 GCGC GCG^C
1697 GCGC
EcoRI 531 GAATTC G^AATTC
681 GAATTC
HaeII 1658 AGCGCC [GA]GCGC^[CT]
1696 AGCGCC
HapII 649 CCGG C^CGG
1910 CCGG
HhaI 1659 GCGC GCG^C
1697 GCGC
HinP1I 1659 GCGC G^CGC
1697 GCGC
HpaII 649 CCGG C^CGG
1910 CCGG
MaeII 1623 ACGT A^CGT
1641 ACGT
MspA1I 706 CAGCTG C[AC]G^C[GT]G
1684 CAGCGG
MspI 649 CCGG C^CGG
1910 CCGG
PstI 526 CTGCAG CTGCA^G
1390 CTGCAG
RsaI 1377 GTAC GT^AC
1427 GTAC
StuI 577 AGGCCT AGG^CCT
755 AGGCCT
TaqI 1719 TCGA T^CGA
1794 TCGA
TthHB8I 1719 TCGA T^CGA
1794 TCGA
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BclI BsaBI BseRI BsiYI
BslI BsmI BsrI BssKI BstF5I
BstNI BstYI DdeI DpnII EarI
EcoRII FbaI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV PleI Sau3AI ScrFI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII