Restriction map for
pF1KE2110
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BbrPI BbsI BcgI BclI BfrI
BpuAI BsaI BsaWI BseAI BsgI
BsiWI BsmBI BsmI BspDI BspEI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoO109I
EcoO65I EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI RsrII SalI SbfI ScaI
SexAI SfcI SfuI SgrAI SmaI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 2483 CGCG CG^CG
Alw44I 1200 GTGCAC G^TGCAC
ApaLI 1200 GTGCAC G^TGCAC
Asp700I 1550 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2660 TGGCCA TGG^CCA
BbeI 2220 GGCGCC GGCGC^C
BglII 1620 AGATCT A^GATCT
BlpI 882 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 882 GCTGAGC GC^T[ACGT]AGC
BsaAI 2187 TACGTA [CT]AC^GT[GA]
BsaBI 2416 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 1699 TGTACA T^GTACA
BsrGI 1699 TGTACA T^GTACA
BssSI 2150 CACGAG CACGAG(-5/-1)
BstUI 2483 CGCG CG^CG
CelII 882 GCTGAGC GC^T[ACGT]AGC
EcoRI 429 GAATTC G^AATTC
EcoRV 2374 GATATC GAT^ATC
KasI 2220 GGCGCC G^GCGCC
KspI 2482 CCGCGG CCGC^GG
MamI 2416 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2660 TGGCCA TGG^CCA
MscI 2660 TGGCCA TGG^CCA
MvnI 2483 CGCG CG^CG
NarI 2220 GGCGCC GG^CGCC
PflMI 477 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 591 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2514 CAGCTG CAG^CTG
SacII 2482 CCGCGG CCGC^GG
SfoI 2220 GGCGCC GGC^GCC
SmlI 1530 CTCAAG C^T[CT][GA]AG
SnaBI 2187 TACGTA TAC^GTA
SspBI 1699 TGTACA T^GTACA
SspI 2206 AATATT AAT^ATT
Van91I 477 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1550 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 551 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1796 CAGTATCTG
AvrII 439 CCTAGG C^CTAGG
1903 CCTAGG
BbvCI 126 CCTCAGC CCTCAGC(-5/-2)
2563 CCTCAGC
BciVI 1929 GGATAC GTATCC(6/5)
2133 GGATAC
BglI 2118 GCCTCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2347 GCCGTGCTGGC
BlnI 439 CCTAGG C^CTAGG
1903 CCTAGG
BsiEI 1438 CGGCCG CG[GA][CT]^CG
2345 CGGCCG
BspMI 257 ACCTGC ACCTGC(4/8)
691 ACCTGC
BstAPI 550 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2269 GCAGCCCATGC
BstYI 1293 AGATCC [GA]^GATC[CT]
1620 AGATCT
CfoI 226 GCGC GCG^C
2221 GCGC
EagI 1438 CGGCCG C^GGCCG
2345 CGGCCG
EclXI 1438 CGGCCG C^GGCCG
2345 CGGCCG
Eco52I 1438 CGGCCG C^GGCCG
2345 CGGCCG
EcoNI 393 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2108 CCTCTTCCAGG
HaeII 225 GGCGCT [GA]GCGC^[CT]
2220 GGCGCC
HgaI 91 GACGC GACGC(5/10)
1685 GACGC
HhaI 226 GCGC GCG^C
2221 GCGC
HinP1I 226 GCGC G^CGC
2221 GCGC
MflI 1293 AGATCC [GA]^GATC[CT]
1620 AGATCT
MseI 1268 TTAA T^TAA
1840 TTAA
NaeI 4 GCCGGC GCC^GGC
2343 GCCGGC
NcoI 662 CCATGG C^CATGG
1457 CCATGG
NgoMIV 4 GCCGGC G^CCGGC
2343 GCCGGC
NspI 479 ACATGC [GA]CATG^[CT]
2155 GCATGT
SacI 21 GAGCTC GAGCT^C
1346 GAGCTC
SapI 18 GAAGAGC GCTCTTC(1/4)
606 GAAGAGC
SfiI 2117 GGCCTCTACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2346 GGCCGTGCTGGCC
StuI 2116 AGGCCT AGG^CCT
2464 AGGCCT
Tru9I 1268 TTAA T^TAA
1840 TTAA
XcmI 2015 CCATTTATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2274 CCATGCCAACACTGG
XhoII 1293 AGATCC [GA]^GATC[CT]
1620 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BsrDI BsrFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII Hin1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I