Restriction map for
pF1KE3552
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AclI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AviII
BamHI BanI BbeI BbrPI BbvCI
BcgI BseAI BsiWI BspDI BspEI
BssHII Bst1107I BstZ17I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoRI
EcoT22I FseI FspI HaeII KasI
KpnI KspI MfeI MroI MunI
NaeI NarI NgoMIV NotI NruI
NsiI PacI PflMI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SfiI SfoI SgrAI SmaI
SnaBI SphI SspI SwaI Van91I
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 11 CGCG CG^CG
AgeI 5507 ACCGGT A^CCGGT
AvrII 1630 CCTAGG C^CTAGG
BciVI 297 GGATAC GTATCC(6/5)
BclI 2445 TGATCA T^GATCA
BcnI 2294 CCGGG CC^[GC]GG
BlnI 1630 CCTAGG C^CTAGG
BsaAI 2695 CACGTA [CT]AC^GT[GA]
BsaWI 5507 ACCGGT [AT]^CCGG[AT]
BsgI 4051 GTGCAG GTGCAG(16/14)
BsiEI 5654 CGACCG CG[GA][CT]^CG
BsmBI 1445 CGTCTC CGTCTC(1/5)
BspLU11I 5336 ACATGT A^CATGT
BsrBI 5472 GAGCGG CCGCTC(-3/-3)
BsrFI 5507 ACCGGT [GA]^CCGG[CT]
BssSI 5639 CACGAG CACGAG(-5/-1)
BstBI 3920 TTCGAA TT^CGAA
BstUI 11 CGCG CG^CG
CfoI 5397 GCGC GCG^C
Cfr10I 5507 ACCGGT [GA]^CCGG[CT]
DraIII 5356 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3054 CCTGTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4470 GATATC GAT^ATC
FbaI 2445 TGATCA T^GATCA
HhaI 5397 GCGC GCG^C
HinP1I 5397 GCGC G^CGC
HpaI 5187 GTTAAC GTT^AAC
MluI 10 ACGCGT A^CGCGT
MvnI 11 CGCG CG^CG
NciI 2294 CCGGG CC^[GC]GG
NheI 3084 GCTAGC G^CTAGC
NspV 3920 TTCGAA TT^CGAA
PaeR7I 2373 CTCGAG C^TCGAG
PinAI 5507 ACCGGT A^CCGGT
SalI 4805 GTCGAC G^TCGAC
SbfI 997 CCTGCAGG CCTGCA^GG
ScaI 705 AGTACT AGT^ACT
SfuI 3920 TTCGAA TT^CGAA
SpeI 4159 ACTAGT A^CTAGT
Sse8387I 997 CCTGCAGG CCTGCA^GG
XbaI 3756 TCTAGA T^CTAGA
XhoI 2373 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1313 GACGTC GACGT^C
1443 GACGTC
AccI 4069 GTAGAC GT^[AC][GT]AC
4805 GTCGAC
AflII 1036 CTTAAG C^TTAAG
3820 CTTAAG
AflIII 10 ACGCGT A^C[GA][CT]GT
5336 ACATGT
AhdI 2749 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2822 GACCCCTTGTC
AlwNI 215 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
4625 CAGGCTCTG
AocI 2208 CCTAAGG CC^T[ACGT]AGG
4816 CCTGAGG
AseI 2550 ATTAAT AT^TAAT
3486 ATTAAT
AsnI 2550 ATTAAT AT^TAAT
3486 ATTAAT
AspEI 2749 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2822 GACCCCTTGTC
AspHI 1436 GAGCAC G[AT]GC[AT]^C
4488 GAGCAC
AvaI 1823 CTCGGG C^[CT]CG[GA]G
2373 CTCGAG
BfrI 1036 CTTAAG C^TTAAG
3820 CTTAAG
BglI 2247 GCCTTTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4666 GCCTCCCTGGC
BglII 1084 AGATCT A^GATCT
1764 AGATCT
BsaBI 2633 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2985 GATAGACATC
BsiHKAI 1436 GAGCAC G[AT]GC[AT]^C
4488 GAGCAC
BsoBI 1823 CTCGGG C^[CT]CG[GA]G
2373 CTCGAG
Bsp1407I 355 TGTACA T^GTACA
2915 TGTACA
BspMI 333 ACCTGC ACCTGC(4/8)
2308 ACCTGC
BsrGI 355 TGTACA T^GTACA
2915 TGTACA
BstAPI 3246 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5806 GCAGCGAATGC
BstXI 1007 CCAGATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5699 CCAGCACCTTGG
Bsu36I 2208 CCTAAGG CC^T[ACGT]AGG
4816 CCTGAGG
DraI 930 TTTAAA TTT^AAA
1747 TTTAAA
Eam1105I 2749 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2822 GACCCCTTGTC
Eco81I 2208 CCTAAGG CC^T[ACGT]AGG
4816 CCTGAGG
FauI 578 CCCGC CCCGC(4/6)
5474 GCGGG
HindIII 560 AAGCTT A^AGCTT
5772 AAGCTT
MamI 2633 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2985 GATAGACATC
NcoI 638 CCATGG C^CATGG
2869 CCATGG
NdeI 2642 CATATG CA^TATG
3498 CATATG
PshAI 4122 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4798 GACTCCAGTC
PshBI 2550 ATTAAT AT^TAAT
3486 ATTAAT
PstI 998 CTGCAG CTGCA^G
5804 CTGCAG
SexAI 717 ACCTGGT A^CC[AT]GGT
1277 ACCAGGT
SspBI 355 TGTACA T^GTACA
2915 TGTACA
StuI 53 AGGCCT AGG^CCT
4664 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI Asp700I AspI AvaII
BalI BanII BbsI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI Hin1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII
XmnI