Restriction map for
pF1KE1851
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspHI AvrII BalI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsiHKAI BsmAI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrDI
BsrGI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoNI
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HindIII HpaI KpnI MaeI
MamI MfeI MluI MluNI MscI
MseI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp509I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 868 GTCTAC GT^[AC][GT]AC
AccIII 290 TCCGGA T^CCGGA
AflIII 454 ACGTGT A^C[GA][CT]GT
Aor51HI 1028 AGCGCT AGC^GCT
AscI 935 GGCGCGCC GG^CGCGCC
AspI 862 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 576 GGACC G^G[AT]CC
AviII 686 TGCGCA TGC^GCA
BamHI 230 GGATCC G^GATCC
BanI 754 GGCGCC G^G[CT][GA]CC
BbeI 754 GGCGCC GGCGC^C
BbsI 829 GAAGAC GAAGAC(2/6)
BpuAI 829 GAAGAC GAAGAC(2/6)
BsaWI 290 TCCGGA [AT]^CCGG[AT]
BseAI 290 TCCGGA T^CCGGA
BseRI 123 CTCCTC GAGGAG(10/8)
BsiWI 619 CGTACG C^GTACG
BspEI 290 TCCGGA T^CCGGA
BspHI 590 TCATGA T^CATGA
BsrBI 59 CCGCTC CCGCTC(-3/-3)
BsrFI 464 ACCGGC [GA]^CCGG[CT]
BssSI 1099 CACGAG CACGAG(-5/-1)
BstEII 402 GGTCACC G^GT[ACGT]ACC
BstPI 402 GGTCACC G^GT[ACGT]ACC
BstYI 230 GGATCC [GA]^GATC[CT]
Cfr10I 464 ACCGGC [GA]^CCGG[CT]
DdeI 429 CTCAG C^T[ACGT]AG
DraIII 238 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1028 AGCGCT AGC^GCT
EcoO65I 402 GGTCACC G^GT[ACGT]ACC
FspI 686 TGCGCA TGC^GCA
HincII 349 GTCAAC GT[CT]^[GA]AC
HindII 349 GTCAAC GT[CT]^[GA]AC
KasI 754 GGCGCC G^GCGCC
KspI 780 CCGCGG CCGC^GG
MflI 230 GGATCC [GA]^GATC[CT]
MroI 290 TCCGGA T^CCGGA
NarI 754 GGCGCC GG^CGCC
PaeR7I 169 CTCGAG C^TCGAG
PflFI 862 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 408 CCATTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 575 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 590 TCATGA T^CATGA
SacII 780 CCGCGG CCGC^GG
SfiI 475 GGCCCCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 754 GGCGCC GGC^GCC
SmaI 478 CCCGGG CCC^GGG
SmlI 169 CTCGAG C^T[CT][GA]AG
Tth111I 862 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 408 CCATTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 169 CTCGAG C^TCGAG
XhoII 230 GGATCC [GA]^GATC[CT]
XmaCI 478 CCCGGG C^CCGGG
XmaI 478 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 231 GATCC GGATC(4/5)
230 GGATC
ApaI 325 GGGCCC GGGCC^C
483 GGGCCC
AvaI 169 CTCGAG C^[CT]CG[GA]G
478 CCCGGG
BbrPI 453 CACGTG CAC^GTG
690 CACGTG
BglI 476 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
533 GCCTCTTCGGC
BpmI 320 CTCCAG CTGGAG(16/14)
820 CTGGAG
BsmFI 575 GGGAC GGGAC(10/14)
311 GTCCC
BsoBI 169 CTCGAG C^[CT]CG[GA]G
478 CCCGGG
BspMI 134 ACCTGC ACCTGC(4/8)
578 ACCTGC
BstF5I 665 GGATG GGATG(2/0)
698 GGATG
DpnII 231 GATC ^GATC
345 GATC
EagI 528 CGGCCG C^GGCCG
540 CGGCCG
EarI 334 CTCTTC CTCTTC(1/4)
535 CTCTTC
EclXI 528 CGGCCG C^GGCCG
540 CGGCCG
Eco52I 528 CGGCCG C^GGCCG
540 CGGCCG
FokI 665 GGATG GGATG(9/13)
698 GGATG
Ksp632I 334 CTCTTC CTCTTC(1/4)
535 CTCTTC
MboI 231 GATC ^GATC
345 GATC
NdeII 231 GATC ^GATC
345 GATC
NlaIII 591 CATG CATG^
677 CATG
PmaCI 453 CACGTG CAC^GTG
690 CACGTG
PmlI 453 CACGTG CAC^GTG
690 CACGTG
PvuII 184 CAGCTG CAG^CTG
1065 CAGCTG
Sau3AI 231 GATC ^GATC
345 GATC
SfaNI 639 GATGC GCATC(5/9)
699 GATGC
TfiI 294 GAATC G^A[AT]TC
445 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanII BbvI BcnI BmyI BsaAI
BsaHI BsaJI BsgI BsiEI BsiYI
BslI Bsp1286I BsrI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV PleI
PstI RsaI Sau96I ScrFI SfcI
TaqI TseI Tsp45I TspRI TthHB8I